Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Purification of hemoglobin by adsorption on nitrogen-doped flower-like carbon superstructures

  • 32 Accesses

Abstract

Nitrogen-doped flower-like carbon superstructures (NPC-F) are prepared via carbonizing self-assembled polyimide nanosheets. SEM, TEM, XPS, and N2 sorption methods are adopted to characterize the flower-like structure. NPC-F exhibits adsorption selectivity for hemoglobin (Hb) because the specific pyridinic N groups of NPC-F could coordinate with the sixth vacancy of ferrous ion in hemoglobin. The adsorption behavior fits well with Langmuir model with a maximum adsorption capacity of 360.0 mg g−1 and the adsorbed Hb could be lightly stripped from the NPC-F nanospheres surface by 0.5 wt% CTAB solution. Circular dichroism spectra indicate no obvious conformation changing of Hb during purification process by NPC-F nanospheres. Five cycles of a continuous adsorption/desorption experiment demonstrate the reusability of NPC-F as adsorbent for Hb. The prepared NPC-F superstructures are then employed for the isolation of Hb from human whole blood sample, obtaining high-purity Hb as demonstrated by sodium dodecyl sulfate polyacrylamide gel electrophoresis assays.

Nitrogen-doped flower-like carbon superstructure (NPC-F) is used to isolate target protein. NPC-F exhibits highly selective capture capacity towards hemoglobin because the specific pyridinic N groups of NPC-F could coordinate with the sixth vacant coordinating position of Fe2+ in hemoglobin.

This is a preview of subscription content, log in to check access.

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Scheme 2
Fig. 4
Fig. 5

References

  1. 1.

    Xu ZX, Zhuang XD, Yang CQ, Cao J, Yao ZX, Tang YP, Jiang JZ, Wu DQ, Feng XL (2016) Nitrogen-doped porous carbon superstructures derived from hierarchical assembly of polyimide nanosheets. Adv Mater 28:1981–1987

  2. 2.

    Zhang Y, Liu JW, Chen XW, Wang JH (2015) A three-dimensional amylopectin-reduced graphene oxide framework for efficient adsorption and removal of hemoglobin. J Mater Chem B 3:983–989

  3. 3.

    Shan CS, Yang HF, Song JF, Han DX, Ivaska A, Niu L (2009) Direct electrochemistry of glucose oxidase and biosensing for glucose based on graphene. Anal Chem 81:2378–2382

  4. 4.

    Chabot V, Higgins D, Yu A, Xiao XC, Chen ZW, Zhang JJ (2014) A review of graphene and graphene oxide sponge: material synthesis and applications to energy and the environment. Energy Environ Sci 7:1564–1596

  5. 5.

    Yang S, Bachman RE, Feng X, Müllen K (2013) Use of organic precursors and graphenes in the controlled synthesis of carbon-containing nanomaterials for energy storage and conversion. Acc Chem Res 46:116–128

  6. 6.

    Choi IY, Lee J, Ahn H, Lee J, Choi HC, Park MJ (2015) High-conductivity two-dimensional polyaniline nanosheets developed on ice surfaces. Angew Chem Int Ed 54:10497–10501

  7. 7.

    Yu Z, Yan H, Lu K, Zhang YJ, Wei ZX (2012) Self-assembly of two-dimensional nanostructures of linear regioregular poly(3-hexylthiophene). RSC Adv 2:338–343

  8. 8.

    Jeon SS, An HH, Yoon CS, Im SS (2011) Synthesis of ultra-thin polypyrrole nanosheets for chemical sensor applications. Polymer 52:652–657

  9. 9.

    Briseno AL, Mannsfeld SCB, Shamberger PJ, Ohuchi FS, Bao Z, Jenekhe SA, Xia Y (2008) Self-assembly, molecular packing, and electron transport in n-type polymer semiconductor nanobelts. Chem Mater 20:4712–4719

  10. 10.

    Saufi SM, Ismail AF (2004) Fabrication of carbon membranes for gas separation-a review. Carbon 42:241–259

  11. 11.

    Shtukenberg AG, Punin YO, Gunn E, Kahr B (2012) Spherulites. Chem Rev 112:1805–1838

  12. 12.

    Granasy L, Pusztai T, Tegze G, Warren JA, Douglas JF (2005) Growth and form of spherulites. Phys Rev E 72:011605

  13. 13.

    Zhang DD, Chen Q, Hu LL, Chen XW, Wang JH (2015) Preparation of a cobalt mono-substituted silicotungstic acid doped with aniline for the selective adsorption of ovalbumin. J Mater Chem B 3:4363–4369

  14. 14.

    Guo PF, Wang XM, Chen XW, Yang T, Chen ML, Wang JH (2019) Nanostructures serve as adsorbents for the selective separation/enrichment of proteins. Trends in Anal Chem No 115650

  15. 15.

    Yuan Y, Tam MF, Simplaceanu V, Ho C (2015) New look at hemoglobin allostery. Chem Rev 115:1702–1724

  16. 16.

    Selvin E, Marinopoulos S, Berkenblit G, Rami T, Brancati FL, Powe NR, Golden SH (2004) Meta-analysis: glycosylated hemoglobin and cardiovascular disease in diabetes mellitus. Ann Intern Med 141:421–431

  17. 17.

    Rees DC, Williams TN, Gladwin MT (2010) Sickle-cell disease. Lancet 376:2018–2031

  18. 18.

    Guo ZY, Zhang Y, Zhang DD, Shu Y, Chen XW, Wang JH (2016) Magnetic nanospheres encapsulated by mesoporous copper oxide shell for selective isolation of hemoglobin. ACS Appl Mater Interfaces 8:29734–29741

  19. 19.

    Li JH, Chen MJ, Gao Z, Du J, Yang WT, Yin MZ (2016) Effective approach towards Si-bilayer-IDA modified CoFe2O4 magnetic nanoparticles for high efficient protein separation. Colloids Surf B 146:468–474

  20. 20.

    Zhang DD, Hu LL, Chen Q, Chen XW, Wang JH (2016) Selective adsorption of hemoglobin with polyoxometalate-derived hybrid by solidification of super-lacunary phosphotungstate polyoxoanions. Talanta 159:23–28

  21. 21.

    Meng H, Chen XW, Wang JH (2011) One-pot synthesis of N, N-bis [2-methylbutyl] imidazolium hexafluorophosphate-TiO2 nanocomposites and application for protein isolation. J Mater Chem 21:14857–14863

  22. 22.

    Zhao G, Chen S, Chen XW, He RH (2013) Selective isolation of hemoglobin by use of imidazolium-modified polystyrene as extractant. Anal Bioanal Chem 405:5353–5358

  23. 23.

    Zhang Y, Xing LG, Chen XW, Wang JH (2015) Nano copper oxide-incorporated mesoporous carbon composite as multimode adsorbent for selective isolation of hemoglobin. ACS Appl Mater Interfaces 7:5116–5123

  24. 24.

    Liu JW, Zhang Y, Chen XW, Wang JH (2014) Graphene oxide-rare earth metal-organic framework composites for the selective isolation of hemoglobin. ACS Appl Mater Interfaces 6:10196–10204

  25. 25.

    Yan J, Wang Q, Wei T, Fan ZJ (2014) Recent advances in design and fabrication of electrochemical supercapacitors with high energy densities. Adv Energy Mater 4:1300816

  26. 26.

    Wu ZS, Yang SB, Sun Y, Parvez K, Feng XL, Mullen K (2012) 3D nitrogen-doped graphene aerogel-supported Fe3O4 nanoparticles as efficient eletrocatalysts for the oxygen reduction reaction. J Am Chem Soc 134:9082–9085

  27. 27.

    Zhang M, Wu YP, Feng XZ, He XW, Chen LX, Zhang YK (2010) Fabrication of mesoporous silica-coated CNTs and application in size-selective protein separation. J Mater Chem 20:5835–5842

  28. 28.

    Liu JW, Zhang Q, Chen XW, Wang JH (2011) Surface assembly of graphene oxide nanosheets on SiO2 particles for the selective isolation of hemoglobin. Chem-Eur J 17:4864–4870

  29. 29.

    Vinu A, Hossian KZ, Srinivasu P, Miyahara M, Anandan S, Gokulakrishnan N, Mori T, Ariga K, Balasubramanian VV (2007) Carboxy-mesoporous carbon and its excellent adsorption capability for proteins. J Mater Chem 17:1819–1825

  30. 30.

    Wei J, Li YH, Wang MH, Yue Q, Sun ZK, Wang C, Zhao YJ, Deng YH, Zhao DY (2013) A systematic investigation of the formation of ordered mesoporous silicas using poly(ethylene oxide)-b-poly(methyl methacrylate) as the template. J Mater Chem A 1:8819–8827

  31. 31.

    Pendashteh A, Palma J, Anderson M, Marcilla R (2017) NiCoMnO4 nanoparticles on N-doped graphene: highly efficient bifunctional electrocatalyst for oxygen reduction/evolution reactions. Appl Catal B-Environ 201:241–252

  32. 32.

    Cui XY, Yang SB, Yan XX, Leng JG, Shuang S, Ajayan PM, Zhang ZJ (2016) Pyridinic-nitrogen-dominated graphene aerogels with Fe-N-C coordination for h Adv Funct Mater ighly efficient oxygen reduction reaction. 26:5708–5717

  33. 33.

    Qian K, Wan JJ, Liu F, Girault HH, Liu BH, Yu CZ (2009) A phospho-directed macroporous alumina-silica nanoreactor with multi-functions. ACS Nano 3:3656–3662

  34. 34.

    Egeberg KD, Springer BA, Martinis SA, Sligar SG, Morikis D, Champion PM (1990) Alteration of sperm whale myoglobin heme axial ligation by site-directed mutagenesis. Biochemistry 29:9783–9791

  35. 35.

    Cheng DH, Chen XW, Shu Y, Wang JH (2008) Selective extraction/isolation of hemoglobin with ionic liquid 1-butyl-3-trimethylsilylimidazolium hexafluorophosphate (BtmsimPF6). Talanta 75:1270–1278

  36. 36.

    Kroll T, Hadt RG, Wilson SA, Lundberg M, Yan JJ, Weng TC, Sokaras D, Alonso-Mori R, Casa D, Upton MH, Hedman B, Hodgson KO, Solomon EI (2014) Resonant inelastic X-ray scattering on ferrous and ferric bis-imidazole porphyrin and cytochrome c: nature and role of the axial methionine-Fe bond. J Am Chem Soc 136:18087–18099

  37. 37.

    Liu JW, Wang MM, Zhang Y, Han L, Chen XW, Wang JH (2014) Polymeric ionic liquid modified reduced graphene oxide as adsorbent for highly selective isolation of acidic protein. RSC Adv 4:61936–61943

  38. 38.

    Zhang Y, Zhuang YT, Shen HY, Chen XW, Wang JH (2017) A super hydrophilic silsesquioxane-based composite for highly selective adsorption of glycoproteins. Microchim Acta 184:1037–1044

  39. 39.

    Xie XY, Wang XR, Xu XM, Sun HJ, Chen XG (2010) Investigation of the interaction between endocrine disruptor bisphenol A and human serum albumin. Chemosphere 80:1075–1080

  40. 40.

    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

  41. 41.

    Wei J, Zhou DD, Sun ZK, Deng YH, Xia YY, Zhao DY (2013) A controllable synthesis of rich nitrogen-doped ordered mesoporous carbon for CO2 capture and supercapacitors. Adv Funct Mater 23:2322–2328

  42. 42.

    Qu LT, Liu Y, Baek JB, Dai LM (2010) Nitrogen-doped graphene as efficient metal-free electrocatalyst for oxygen reduction in fuel cells. ACS Nano 4:1321–1326

Download references

Funding

The authors received financial support from the National Natural Science Foundation of China (21675019, 21874014, 21727811).

Author information

Correspondence to Ming-Li Chen or Jian-Hua Wang.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests and there is no compliance with ethical standard.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(DOC 103 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Wang, X., Hu, Z., Guo, P. et al. Purification of hemoglobin by adsorption on nitrogen-doped flower-like carbon superstructures. Microchim Acta 187, 162 (2020). https://doi.org/10.1007/s00604-020-4151-9

Download citation

Keywords

  • Nitrogen-doped materials
  • Flower-like structure
  • Pyridinic N
  • Hemoglobin enrichment
  • Coordination chemistry