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Microchimica Acta

, 187:10 | Cite as

An anti-BSA antibody-based immunochromatographic assay for chloramphenicol and aflatoxin M1 by using carboxy-modified CdSe/ZnS core–shell nanoparticles as label

  • Zhiwei Qie
  • Wenliang Yan
  • Zichen Gao
  • Wu Meng
  • Rui Xiao
  • Shengqi WangEmail author
Original Paper
  • 26 Downloads

Abstract

A lateral-flow immunochromatographic assay with excellent sensitivity and wide application potential is described. The bovine serum albumin (BSA) antibody was immobilized in the test line for universality, and preincubation was introduced for high method sensitivity. Carboxy-modified CdSe/ZnS core–shell nanoparticles were used as label, and the fluorescence peaking at 605 nm was detected. The fluorescence in the test line was negative against the relevant analyte content. The chloramphenicol (CAP) and the aflatoxin M1 (AFM1) in milk were detected using the same strip to validate the universality. After optimization, the detection limit for CAP is 10 pg·mL−1, which is three times less that of a conventional assay (30 pg·mL−1). The detection limit for AFM1 was 6 pg·mL−1, which was 13 times less than that of a conventional assay (8 pg·mL−1). The method was applied in the analysis of spiked milk samples. The performance was compared with that of the commercial ELISA kit, and good agreement was observed.

Graphical abstract

Schematic representation of the universal and sensitive combined immunochromatographic assay (USICA) and conventional immunochromatographic assay (TICA) of chloramphenicol (CAP) and aflatoxin M1.

Keywords

Sensitivity Universality Immunoassay Competitive recognition Pre-incubation Mycotoxin Milk 

Notes

Acknowledgments

We thank our colleagues for their contributions to this manuscript. This work was supported by the Major Infectious Diseases, such as the AIDS and Viral Hepatitis Prevention and Control Technology Major Projects (2018ZX10712-001), and the Major Projects (No. AWS16J020).

Supplementary material

604_2019_4009_MOESM1_ESM.docx (22 kb)
ESM 1 An ESM on QB modification with antitarget mAb and the optimal parameters of CAP- and AFM1-spiked samples using different ICA systems (DOCX 21.7 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Zhiwei Qie
    • 1
  • Wenliang Yan
    • 1
  • Zichen Gao
    • 2
  • Wu Meng
    • 2
  • Rui Xiao
    • 1
  • Shengqi Wang
    • 1
    Email author
  1. 1.Beijing Institute of Radiation MedicineBeijingPeople’s Republic of China
  2. 2.Center for Diseases Prevention and Control of Rocket ForceBeijingPeople’s Republic of China

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