Fluorometric determination of fipronil by integrating the advantages of molecularly imprinted silica and carbon quantum dots
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A fluorometric method is described for the determination of fipronil, a frequently-used insecticide. It exploits the blue fluorescence of carbon quantum dots (CQDs) and the selectivity of molecularly imprinted silica (MIS). The MIS was prepared via the sol-gel method by using fipronil as the template, 3-aminopropyltriethoxysilane as functional monomer, and tetraethoxysilane as cross-linker in the presence of CQDs. The blue fluorescence of the CQD@MIS, with excitation/emission peaks at 340/422 nm, is quenched by fipronil. The assay works in the 0. 70 pM to 47 μM fipronil concentration range, and the limit of detection is 19 pM. The method was successfully applied to the quantitation of fipronil in spiked eggs, milk, and tap water. Recoveries between 83.8 and 114.0% were achieved. The corresponding relative standard deviations (RSD) are less than 6.67%.
KeywordsFood safety Fluorescence quenching Milk Analysis Egg Analysis Insecticide
The authors are grateful for the financial support provided by the Ministry of Science and Technology of China (Project No.2016YFD0401101) and the National Natural Science Foundation of China (Project No.21375094).
- 9.Peng XT, Li YN, Xia H, Peng LJ, Feng YQ (2016) Rapid and sensitive detection of fipronil and its metabolites in edible oils by solid-phase extraction based on humic acid bonded silica combined with gas chromatography with electron capture detection. J Sep Sci 39(11):2196–2203. https://doi.org/10.1002/jssc.201501250 CrossRefPubMedGoogle Scholar
- 10.Li X, Chen J, He X, Wang Z, Wu D, Zheng X, Zheng L, Wang B (2019) Simultaneous determination of neonicotinoids and fipronil and its metabolites in environmental water from coastal bay using disk-based solid-phase extraction and high-performance liquid chromatography-tandem mass spectrometry. Chemosphere 234:224–231. https://doi.org/10.1016/j.chemosphere.2019.05.243 CrossRefPubMedGoogle Scholar
- 11.Ma J, Lu X, Xia Y, Yan F (2015) Determination of pyrazole and pyrrole pesticides in environmental water samples by solid-phase extraction using multi-walled carbon nanotubes as adsorbent coupled with high-performance liquid chromatography. J Chromatogr Sci 53(2):380–384. https://doi.org/10.1093/chromsci/bmu055 CrossRefPubMedGoogle Scholar
- 12.Wang K, Vasylieva N, Wan D, Eads DA, Yang J, Tretten T, Barnych B, Li J, Li QX, Gee SJ, Hammock BD, Xu T (2018) Quantitative detection of Fipronil and Fipronil-Sulfone in sera of black-tailed prairie dogs and rats after Oral exposure to Fipronil by camel single-domain antibody-based immunoassays. Anal Chem. 91(2):1532-1540. https://doi.org/10.1021/acs.analchem.8b04653 CrossRefGoogle Scholar
- 19.Liu H, Wu D, Zhou K, Wang J, Sun B (2016) Development and applications of molecularly imprinted polymers based on hydrophobic CdSe/ZnS quantum dots for optosensing of N(epsilon)-carboxymethyllysine in foods. Food Chem 211:34–40. https://doi.org/10.1016/j.foodchem.2016.05.038 CrossRefPubMedGoogle Scholar
- 25.Wang Y, Yang Y, Liu W, Ding F, Zhao Q, Zou P, Wang X, Rao H (2018) Colorimetric and fluorometric determination of uric acid based on the use of nitrogen-doped carbon quantum dots and silver triangular nanoprisms. Microchim Acta 185(6):281. https://doi.org/10.1007/s00604-018-2814-6 CrossRefGoogle Scholar
- 26.Fu JW, Xu Q, Chen JF, Chen ZM, Huang XB, Tang XZ (2010) Controlled fabrication of uniform hollow core porous shell carbon spheres by the pyrolysis of core/shell polystyrene/cross-linked polyphosphazene composites. Chem Commun 46(35):6563–6565. https://doi.org/10.1039/c0cc01185a CrossRefGoogle Scholar