Microchimica Acta

, 186:821 | Cite as

A glassy carbon electrode modified with a nanocomposite prepared from Pd/Al layered double hydroxide and carboxymethyl cellulose for voltammetric sensing of hydrogen peroxide

  • Gozal Fazli
  • Sedigheh Esmaeilzadeh BahabadiEmail author
  • Laleh Adlnasab
  • Hamid Ahmar
Original Paper


A Pd/Al layered double hydroxide/carboxymethyl cellulose nanocomposite (CMC@Pd/Al-LDH) was fabricated using carboxymethyl cellulose as a green substrate via co-precipitation method. The synthesized nanocomposite was characterized using different methods such as scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray powder diffraction, transmission electron microscopy, and electrochemical techniques. A glassy carbon electrode (GCE) was then modified with the suspended composite to obtain an electrochemical sensor for hydrogen peroxide (H2O2). The voltammetric (cathodic) current of the modified GCE was measured at −380 mV (vs. Ag/AgCl), at the scan rate of 50 mV.s−1. Results show a linear dynamic range of 1 to 120 μM, and a 0.3 µM limit of detection (at S/N = 3). Intraday and interday relative standard deviations are in the ranges of 4.9–5.4% and 6.8–7.3%, respectively. The sensor was applied for the determination of H2O2 in basil extracts, milk, and spiked river water samples. The recoveries are between 96.60 and 102.30%.

Graphical abstract

A Pd/Al layered double hydroxide/carboxymethyl cellulose nanocomposite (CMC@Pd/Al-LDH) was fabricated via co-precipitation method and was characterized using scanning electron microscopy, Energy-dispersive X-ray spectroscopy, X-ray powder diffraction, transmission electron microscopy and electrochemical techniques. CMC@Pd/Al-LDH was used to fabricate H2O2 electrochemical sensor.


H2O2 Electrochemical sensor Co-precipitation synthesis Modified electrode Electro-catalytic reduction Basil extract Milk sample Differential pulse voltammetry Optimization 



This work was supported by University of Zabol [Grant number: UOZ-GR-9618-20].

Compliance with ethical standards

Conflict of interest

 The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3967_MOESM1_ESM.docx (580 kb)
ESM 1 (DOCX 579 kb)


  1. 1.
    Benvidi A, Nafar MT, Jahanbani S, Tezerjani MD, Rezaeinasab M, Dalirnasab S (2017) Developing an electrochemical sensor based on a carbon paste electrode modified with nano-composite of reduced graphene oxide and CuFe2O4 nanoparticles for determination of hydrogen peroxide. Mater Sci Eng C 75:1435–1447CrossRefGoogle Scholar
  2. 2.
    Bian X, Guo K, Liao L, Xiao J, Kong J, Ji C, Liu B (2012) Nanocomposites of palladium nanoparticle-loaded mesoporous carbon nanospheres for the electrochemical determination of hydrogen peroxide. Talanta 99:256–261CrossRefGoogle Scholar
  3. 3.
    Li Q, Gao W, Zhang X, Liu H, Dou Li G, Li Y, Wang Z, Liu H (2017) Green synthesis of palladium nanoparticles with carboxymethyl cellulose for degradation of azo-dyes. Mater Chem Phys 187:133–140CrossRefGoogle Scholar
  4. 4.
    Devasenathipathy R, Liu YX, Yang C, Wang SF (2017) Simple electrochemical growth of copper nanoparticles decorated silver nanoleaves for the sensitive determination of hydrogen peroxide in clinical lens cleaning solutions. Sensors Actuators B Chem 252:862–869CrossRefGoogle Scholar
  5. 5.
    Gao C, Tian Y, Zhang R, Jing J, Zhang X (2017) Endoplasmic reticulum-directed ratiometric fluorescent probe for quantitive detection of basal H2O2. Anal Chem 89:12945–12950CrossRefGoogle Scholar
  6. 6.
    Ding H, Tang Z, Zhang L, Dong Y (2019) Electrogenerated chemiluminescence of black phosphorus nanosheets and its application in the detection of H2O2. Analyst 144:1326–1333CrossRefGoogle Scholar
  7. 7.
    Xie JX, Chen WJ, Wu XX, Wu YY, Lin H (2017) Enhanced luminol chemiluminescence by co–Fe LDH nanoplates and its application in H2O2 and glucose detection. Anal Methods 9:974–979CrossRefGoogle Scholar
  8. 8.
    Cui M, Zhou J, Zhao Y, Song Q (2017) Facile synthesis of iridium nanoparticles with superior peroxidase-like activity for colorimetric determination of H2O2 and xanthine. Sensors Actuators B Chem 243:203–210CrossRefGoogle Scholar
  9. 9.
    Wang Y, Zhang D, Wang J (2018) Metastable α-AgVO3 microrods as peroxidase mimetics for colorimetric determination of H2O2. Microchim Acta 185(1):1–8CrossRefGoogle Scholar
  10. 10.
    Rui Q, Komori K, Tian Y, Liu H, Luo Y, Sakai Y (2010) Electrochemical biosensor for the detection of H2O2 from living cancer cells based on ZnO nanosheets. Anal Chim Acta 670:57–62CrossRefGoogle Scholar
  11. 11.
    Chen W, Cai S, Ren QQ, Wen W, Zhao YD (2012) Recent advances in electrochemical sensing for hydrogen peroxide: a review. Analyst 137:49–58CrossRefGoogle Scholar
  12. 12.
    Ensafi AA, Abarghoui MM, Rezaei B (2014) Electrochemical determination of hydrogen peroxide using copper/porous silicon based non-enzymatic sensor. Sensors Actuators B Chem 196:398–405CrossRefGoogle Scholar
  13. 13.
    Periasamy AP, Ho YH, Chen SM (2011) Multiwalled carbon nanotubes dispersed in carminic acid for the development of catalase based biosensor for selective amperometric determination of H2O2 and iodate. Biosens Bioelectron 29:151–158CrossRefGoogle Scholar
  14. 14.
    Chen H, Hu L, Chen M, Yan Y, Wu L (2014) Nickel–cobalt layered double hydroxide Nanosheets for high-performance Supercapacitor electrode materials. Adv Funct Mater 24:934–942CrossRefGoogle Scholar
  15. 15.
    Mishra G, Dash B, Pandey S (2018) Layered double hydroxides: a brief review from fundamentals to application as evolving biomaterials. Appl Clay Sci 153:172–186CrossRefGoogle Scholar
  16. 16.
    Li M, Zhu JE, Zhang L, Chen X, Zhang H, Zhang F, Xu S, Evans DG (2011) Facile synthesis of NiAl-layered double hydroxide/graphene hybrid with enhanced electrochemical properties for detection of dopamine. Nanoscale 3:4240–4246CrossRefGoogle Scholar
  17. 17.
    Sprick C, Chede S, Oyanedel-Craver V, Escobar IC (2018) Bio-inspired immobilization of casein-coated silver nanoparticles on cellulose acetate membranes for biofouling control. J Environ Chem Eng 6:2480–2491CrossRefGoogle Scholar
  18. 18.
    Ahmar H, Keshipour S, Hosseini H, Fakhari AR, Shaabani A, Bagheri A (2013) Electrocatalytic oxidation of hydrazine at glassy carbon electrode modified with ethylenediamine cellulose immobilized palladium nanoparticles. J Electroanal Chem 690:96–103CrossRefGoogle Scholar
  19. 19.
    Lang X, Xing-Cai W, Jun-Jie Z (2008) Green preparation and catalytic application of Pd nanoparticles. Nanotechnology 19:1–6Google Scholar
  20. 20.
    Palanisamy S, Velusamy V, Ramaraj S, Chen SW, Yang TCK, Balu S, Banks CE (2019) Facile synthesis of cellulose microfibers supported palladium nanospindles on graphene oxide for selective detection of dopamine in pharmaceutical and biological samples. Mater Sci Eng C 98:256–265CrossRefGoogle Scholar
  21. 21.
    Razmi H, Mohammad-Rezaei R, Heidari H (2009) Self-prussian blue nanoparticles based electrochemical sensor for high sensitive determination of H2O2 in acidic media. Electroanalysis 21:2355–2362CrossRefGoogle Scholar
  22. 22.
    Mattoussi M, Matoussi F, Raouafi N (2018) Non-enzymatic amperometric sensor for hydrogen peroxide detection based on a ferrocene-containing cross-linked redox-active polymer. Sensors Actuators B Chem 274:412–418CrossRefGoogle Scholar
  23. 23.
    Zhao W, Wang H, Qin X, Wang X, Zhao Z, Miao Z et al (2009) A novel nonenzymatic hydrogen peroxide sensor based on multi-wall carbon nanotube/silver nanoparticle nanohybrids modified gold electrode. Talanta 80:1029–1033CrossRefGoogle Scholar
  24. 24.
    Yang X, Ouyang Y, Wu F, Hu Y, Ji Y, Wu Z (2017) Size controllable preparation of gold nanoparticles loading on graphene sheets@ cerium oxide nanocomposites modified gold electrode for nonenzymatic hydrogen peroxide detection. Sensors Actuators B Chem 238:40–47CrossRefGoogle Scholar
  25. 25.
    Pang P, Yang Z, Xiao S, Xie J, Zhang Y, Gao Y (2014) Nonenzymatic amperometric determination of hydrogen peroxide by graphene and gold nanorods nanocomposite modified electrode. J Electroanal Chem 727:27–33CrossRefGoogle Scholar
  26. 26.
    Xin Y, Fu-bing X, Hong-wei L, Feng W, Di-zhao C, Zhao-yang W (2013) A novel H2O2 biosensor based on Fe3O4–au magnetic nanoparticles coated horseradish peroxidase and graphene sheets–Nafion film modified screen-printed carbon electrode. Electrochim Acta 109:750–755CrossRefGoogle Scholar
  27. 27.
    Cao X, Wang N, Wang L, Mo C, Xu Y, Cai X, Guo L (2010) A novel non-enzymatic hydrogen peroxide biosensor based on ultralong manganite MnOOH nanowires. Sensors Actuators B Chem 147:730–734CrossRefGoogle Scholar
  28. 28.
    Long L, Liu X, Chen L, Li D, Jia J (2019) A hollow CuOx/NiOy nanocomposite for amperometric and non-enzymatic sensing of glucose and hydrogen peroxide. Microchim Acta 186:74–84CrossRefGoogle Scholar
  29. 29.
    Li Y, Liu J, Fu Y, Xie Q, Li Y (2019) Magnetic-core@dual-functional-shell nanocomposites with peroxidase mimicking properties for use in colorimetric and electrochemical sensing of hydrogen peroxide. Microchim Acta 186:456–464CrossRefGoogle Scholar
  30. 30.
    Sivakumar M, Veeramani V, Chen SM, Madhu R, Liu SB (2019) Porous carbon-NiO nanocomposites for amperometric detection of hydrazine and hydrogen peroxide. Microchim Acta 186:59–66CrossRefGoogle Scholar
  31. 31.
    Song H, Zhao H, Zhang X, Xu Y, Cheng X, Gao S, Huo L (2019) A hollow urchin-like α-MnO2 as an electrochemical sensor for hydrogen peroxide and dopamine with high selectivity and sensitivity. Microchim Acta 186:210–221CrossRefGoogle Scholar
  32. 32.
    Li Z, Jiang Y, Wang Z, Wang W, Yuan Y, Wu X., ... & Wang D (2018) Nitrogen-rich core-shell structured particles consisting of carbonized zeolitic imidazolate frameworks and reduced graphene oxide for amperometric determination of hydrogen peroxide. Microchim Acta 185:501–509.Google Scholar
  33. 33.
    Tang J, Huang L, Cheng Y, Zhuang J, Li P, Tang D (2018) Nonenzymatic sensing of hydrogen peroxide using a glassy carbon electrode modified with graphene oxide, a polyamidoamine dendrimer, and with polyaniline deposited by the Fenton reaction. Microchim Acta 185:569–577CrossRefGoogle Scholar
  34. 34.
    Sha R, Vishnu N, Badhulika S (2018) Bimetallic Pt-Pd nanostructures supported on MoS2 as an ultra-high performance electrocatalyst for methanol oxidation and nonenzymatic determination of hydrogen peroxide. Microchim Acta 185:399–409CrossRefGoogle Scholar
  35. 35.
    Lyu YP, Wu YS, Wang TP, Lee CL, Chung MY, Lo CT (2018) Hydrothermal and plasma nitrided electrospun carbon nanofibers for amperometric sensing of hydrogen peroxide. Microchim Acta 185:371–377CrossRefGoogle Scholar
  36. 36.
    Su Y, Zhou X, Long Y, Li W (2018) Immobilization of horseradish peroxidase on amino-functionalized carbon dots for the sensitive voltammetric detection of hydrogen peroxide. Microchim Acta 185:114–121CrossRefGoogle Scholar
  37. 37.
    Jahanbakhshi M (2018) Myoglobin immobilized on mesoporous carbon foam in a hydrogel (selep) dispersant for voltammetric sensing of hydrogen peroxide. Microchim Acta 185:121–128CrossRefGoogle Scholar
  38. 38.
    Liu J, Yang C, Shang Y, Zhang P, Liu J, Zheng J (2018) Preparation of a nanocomposite material consisting of cuprous oxide, polyaniline and reduced graphene oxide, and its application to the electrochemical determination of hydrogen peroxide. Microchim Acta 185:172–179CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Gozal Fazli
    • 1
  • Sedigheh Esmaeilzadeh Bahabadi
    • 1
    Email author
  • Laleh Adlnasab
    • 2
  • Hamid Ahmar
    • 3
  1. 1.Department of Biology, Faculty of ScienceUniversity of ZabolZabolIran
  2. 2.Department of Chemistry, Chemistry and Petrochemistry Research CenterStandard Research InstituteKarajIran
  3. 3.Department of Chemistry, Faculty of ScienceUniversity of ZabolZabolIran

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