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Microchimica Acta

, 186:785 | Cite as

A turn-on graphene quantum dot and graphene oxide based fluorometric aptasensor for the determination of telomerase activity

  • Elahe Kazemi
  • Habib BagheriEmail author
  • Dariush Norouzian
Original Paper
  • 55 Downloads

Abstract

A turn-on fluorometric assay is described for determination of the activity of enzyme telomerase. For this purpose, graphene quantum dots (GQDs) were first modified with the telomeric sequence (5′-amino-AATCCGTCGAGCAGAGTT-3′) via a condensation reaction. Injection of graphene oxide causes instant quenching of the blue fluorescence of the GQDs. Addition of cell extract containing telomerase, triggers the extension of telomer via addition of specific sequence (TTAGGG)n to its 3′ end. Fluorescence, best measured at excitation/emission wavelengths of 390/446 nm, is subsequently restored due to folding of the extended telomeric sequence into G-quadruplex structure. The method was applied to the determination of telomerase activity in crude cell extracts of as little as 10 HeLa cells. The linear dynamic range extends from 10 to 6500 cells.

Graphical abstract

In this study, a new turn-on graphene quantum dotm and graphene oxide based fluorometric assay is developed for the determination of telomerase activity

Keywords

Optical sensing Fluorescence Biosensors Nanoprobe Biomarker Cancer detection G-quadruplex 

Notes

Acknowledgments

This work was supported by the grant number of 95008282 from the Iran National Science Foundation (INSF). The Research Council and Graduates School of Sharif University of Technology (SUT) are also thanked for supporting the project.

Compliance with ethical standards

Competing interests

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3956_MOESM1_ESM.docx (178 kb)
ESM 1 (DOCX 177 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Environmental and Bio-Analytical Laboratories, Department of ChemistrySharif University of TechnologyTehranIran
  2. 2.Pilot Nanobiotechnology DepartmentPasteur Institute of IranTehranIran

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