A turn-on graphene quantum dot and graphene oxide based fluorometric aptasensor for the determination of telomerase activity
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A turn-on fluorometric assay is described for determination of the activity of enzyme telomerase. For this purpose, graphene quantum dots (GQDs) were first modified with the telomeric sequence (5′-amino-AATCCGTCGAGCAGAGTT-3′) via a condensation reaction. Injection of graphene oxide causes instant quenching of the blue fluorescence of the GQDs. Addition of cell extract containing telomerase, triggers the extension of telomer via addition of specific sequence (TTAGGG)n to its 3′ end. Fluorescence, best measured at excitation/emission wavelengths of 390/446 nm, is subsequently restored due to folding of the extended telomeric sequence into G-quadruplex structure. The method was applied to the determination of telomerase activity in crude cell extracts of as little as 10 HeLa cells. The linear dynamic range extends from 10 to 6500 cells.
KeywordsOptical sensing Fluorescence Biosensors Nanoprobe Biomarker Cancer detection G-quadruplex
This work was supported by the grant number of 95008282 from the Iran National Science Foundation (INSF). The Research Council and Graduates School of Sharif University of Technology (SUT) are also thanked for supporting the project.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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