Microchimica Acta

, 186:755 | Cite as

Rapid colorimetric determination of dopamine based on the inhibition of the peroxidase mimicking activity of platinum loaded CoSn(OH)6 nanocubes

  • Hao Liu
  • Ya-Nan Ding
  • Bing Bian
  • Lei Li
  • Ruomeng Li
  • Xianxi Zhang
  • Zhenxue Liu
  • Xiao Zhang
  • Gaochao Fan
  • Qingyun LiuEmail author
Original Paper


Platinum nanoparticles were loaded on CoSn(OH)6 nanocubes via a co-precipitation method. The material (NCs) is shown to be a viable peroxidase mimic that catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2) to generate oxidized TMB (oxTMB) with absorption at 652 nm. The formation of the blue color can be observed in <30 s. Thus, a visual and colorimetric assay was worked out for H2O2. It has a detection limit as low as 4.4 μM and works in the 5 to 200 μM concentration range. The method was also used to detect dopamine (DA) which is found to inhibit the enzyme mimicking activity of the NCs. Hence, less blue color is formed in its presence. The respective DA assay has a linear response in the 5.0 to 60 μM concentration range and a 0.76 μM detection limit.

Graphical abstract

Schematic diagram of a visual colorimetric method for determination of H2O2 and dopamine (DA) with the aid of color change of 3,3′,5,5′-tetramethylbenzidine (oxTMB), based on the peroxidase-like activity of Pt/CoSn(OH)6 nanocubes.


Synergistic effect Co-precipitation Antioxidants Inhibition Catalytic mechanism Serum 



This work was supported by the National Natural Science Foundation of China (Grant No. 21971152), Scientific Research Foundation of Shandong University of Science and Technology for Recruited Talents (Grant No. 2015RCJJ018, 2017RCJJ040 and 2017RCJJ041), Natural Science Foundation of Shandong Province (Grant No. ZR2018MB002, ZR2018MEE003, ZR2018PEE006 and ZR2017BB008), the Science and Technology Projects for Colleges and Universities in Shandong Province (No. J17KA097) and Innovation Fund of Science & Technology of Graduate Students (SDKDYC180239).

Compliance with ethical standards

Conflict of interest

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3940_MOESM1_ESM.docx (210 kb)
ESM 1 (DOCX 210 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Chemical and Environmental Engineering; State Key Laboratory of Mining Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and TechnologyShandong University of Science and TechnologyQingdaoChina
  2. 2.College of Chemistry and Molecular EngineeringQingdao University of Science & TechnologyQingdaoChina
  3. 3.Shandong Provincial Key Laboratory/Collaborative Innovation Center of Chemical Energy Storage & Novel Cell TechnologyLiaocheng UniversityLiaochengChina
  4. 4.School of Chemistry and Chemical EngineeringLiaocheng UniversityLiaochengChina

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