Microchimica Acta

, 186:799 | Cite as

Fibrinogen-templated gold nanoclusters for fluorometric determination of cysteine and mercury(II)

  • Zhiguang Suo
  • Xialing Hou
  • Ziheng Hu
  • Yihao Liu
  • Feifei Xing
  • Lingyan FengEmail author
Original Paper


Gold nanoclusters (Au NCs) using fibrinogen (FBG) protein as template are fabricated via one-pot reduction strategy, and applied for fluorometric detections of cysteine (Cys) and mercury(II). The modified FBG-Au NCs exhibit red fluorescence, with excitation/emission maxima at 360/620 nm, a 7% quantum yield, and a 2.2 μs decay time. The fluorescence of the nanoprobe is quenched by Cys and Hg(II). Cys can be determined by fluorometry in the 0.01 to 150 μmol L−1 concentration range and with a detection limit of 0.79 μmol L−1. Due to the oxidation of Hg(II), it can be detected in the 0.01 to 10 μmol L−1 concentration range. The properties of the FBG-Au NCs and the analytical performance are comparable with previously reported peptide/protein-templated Au NCs, supplying a promising candidate for Au NCs nanoprobes synthesis and applications.

Graphical abstract

Schematic representation of the preparation of gold nanoclusters (Au NCs) using fibrinogen (FBG) as the template. The modified Au NCs were applied to the fluorometric detection of cysteine (Cys) and mercury ion (Hg(II)).


Fluorescent nanoprobes Au NCs Static quenching Ultra-stability Quantum yield 



The authors gratefully acknowledge Shanghai Sailing Program (17YF1405700), the National Natural Science Foundation of China (Grant No. 21705106), as well as the support of the Program for Professor of Special Appointment (Eastern Scholar) at the Shanghai Institution of Higher Learning (No. TP2016023) and Shanghai Natural Science Foundation (No. 18ZR1415400).

Supplementary material

604_2019_3919_MOESM1_ESM.pdf (181 kb)
ESM 1 (PDF 180 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Materials Genome Institute, and Department of Chemistry, College of ScienceShanghai UniversityShanghaiChina

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