Microchimica Acta

, 186:807 | Cite as

Carbon fibers coated with urchin-like copper sulfide for nonenzymatic voltammetric sensing of glucose

  • Murugan Keerthi
  • Bhuvanenthiran Mutharani
  • Shen-Ming ChenEmail author
  • Palraj Ranganathan
Original Paper


Urchin-like CuS was grown on xanthan gum-derived carbon nanofibers to obtain a sensor for enzyme-free electrochemical sensing of glucose. The unique nanostructure of the sensor provides a large specific surface, more electrocatalytically active sites, and high electrical conductivity. The voltammetric response to glucose, best measured at around 57 mV (vs. Ag/AgCl (E/V)) in 0.1 M NaOH solution, covers two linear ranges, one from 0.1–125 μM, another from 0.16 to 1.2 mM. The sensitivity is quite high (23.7 μA mM−1 cm−2), and the detection limit is low (19 nM at S/N = 3). The sensor has high selectivity against potentially interfering molecules such as fructose, appreciable operational stability, excellent durability, and good repeatability (with relative standard deviations of 2.3%). It was successfully applied to the determination of glucose in diluted serum samples.

Graphical abstract

Schematic representation of electrochemical detection of glucose based on the use of a screen printed carbon electrode (SPCE) modified with CuS and xanthan gum-derived carbon nanofibers (XGCNFs).


Xanthan gum derived carbon Copper sulfide Electrochemical detection Enzyme-free biosensor Human blood serum 



This project was supported by the Ministry of Science and Technology (MOST 106-2113-M-027-003), Taiwan, ROC.

Compliance with ethical standards

Conflict of interest

There are no conflicts of interest to declare.

Supplementary material

604_2019_3915_MOESM1_ESM.docx (358 kb)
ESM 1 (DOCX 358 kb)


  1. 1.
    Bae CW, Toi PT, Kim BY, Lee WI, Lee HB, Hanif A, Lee E.H, Lee NE (2019) Fully stretchable capillary microfluidics-integrated Nanoporous gold electrochemical sensor for wearable continuous glucose monitoring. ACS Appl Mater Interfaces 11(16): 14567–14575CrossRefGoogle Scholar
  2. 2.
    Hu C, Yang DP, Zhu F, Jiang F, Shen S, Zhang J (2014) Enzyme-labeled Pt@ BSA nanocomposite as a facile electrochemical biosensing interface for sensitive glucose determination. ACS Appl Mater Interfaces 6:4170–4178CrossRefGoogle Scholar
  3. 3.
    Gao H, Xiao F, Ching CB, Duan H (2011) One-step electrochemical synthesis of PtNi nanoparticle-graphene nanocomposites for nonenzymatic amperometric glucose detection. ACS Appl Mater Interfaces 3:3049–3057CrossRefGoogle Scholar
  4. 4.
    Tomanin PP, Cherepanov PV, Besford QA, Christofferson AJ, Amodio A, McConville CF, Yarovsky I, Caruso F, Cavalieri F (2018) Cobalt phosphate nanostructures for non-enzymatic glucose sensing at physiological pH. ACS Appl Mater Interfaces 10:42786–42795CrossRefGoogle Scholar
  5. 5.
    Dhara K, Mahapatra DR (2018) Electrochemical nonenzymatic sensing of glucose using advanced nanomaterials. Microchim Acta 185:49CrossRefGoogle Scholar
  6. 6.
    Deepalakshmi T, Tran DT, Kim NH, Chong KT, Lee JH (2018) Nitrogen-doped Graphene-encapsulated nickel cobalt nitride as a highly sensitive and selective electrode for glucose and hydrogen peroxide sensing applications. ACS Appl Mater Interfaces 10:35847–35858CrossRefGoogle Scholar
  7. 7.
    Chen X, Liu D, Cao G, Tang Y, Wu C (2019) In situ synthesis of Sandwich-like Graphene@ ZIF-67 Heterostructure for highly sensitive nonenzymatic glucose sensing in human serums. ACS Appl Mater Interfaces 11(9):9374–9384CrossRefGoogle Scholar
  8. 8.
    Lin LY, Karakocak BB, Kavadiya S, Soundappan T, Biswas P (2018) A highly sensitive non-enzymatic glucose sensor based on cu/Cu2O/CuO ternary composite hollow spheres prepared in a furnace aerosol reactor. Sensors Actuators B Chem 259:745–752CrossRefGoogle Scholar
  9. 9.
    Qian L, Mao J, Tian X, Yuan H, Xiao D (2013) In situ synthesis of CuS nanotubes on cu electrode for sensitive nonenzymatic glucose sensor. Sensors Actuators B Chem 176:952–959CrossRefGoogle Scholar
  10. 10.
    Wang S, Han Z, Li Y, Peng R, Feng B (2015) An electrochemical sensor based on reduced graphene oxide and copper sulfide hollow nanospheres. RSC Adv 5:107318–107325CrossRefGoogle Scholar
  11. 11.
    Siahrostami S (2018) Designing carbon-based materials for efficient electrochemical reduction of CO2. Ind Eng Chem Res 58:879–885CrossRefGoogle Scholar
  12. 12.
    De B, Balamurugan J, Kim NH, Lee JH (2017) Enhanced electrochemical and Photocatalytic performance of Core–Shell CuS@carbon quantum dots@carbon hollow Nanospheres. ACS Appl Mater Interfaces 9:2459–2246CrossRefGoogle Scholar
  13. 13.
    Elschner T, Heinze T (2015) Cellulose carbonates: a platform for promising biopolymer derivatives with multifunctional capabilities. Macromol Biosci 15:735–746CrossRefGoogle Scholar
  14. 14.
    Cheng Y, Pang K, Wu X, Zhang Z, Xu X, Ren J, Huang W, Song R (2018) In situ hydrothermal synthesis MoS2/guar gum carbon Nanoflowers as advanced Electrocatalysts for Electrocatalytic hydrogen evolution. ACS Sustain Chem Eng 6:8688–8696CrossRefGoogle Scholar
  15. 15.
    Lin Y, Chen Z, Yu C, Zhong W (2019) Heteroatom-doped Sheet-like and hierarchical porous carbon based on natural biomass small molecule peach gum for high-performance Supercapacitors. ACS Sustain Chem Eng 7:3389–3403CrossRefGoogle Scholar
  16. 16.
    Jiang L, Sheng L, Fan Z (2018) Biomass-derived carbon materials with structural diversities and their applications in energy storage. Sci Chin Mater 61:133–158CrossRefGoogle Scholar
  17. 17.
    Palaniraj A, Jayaraman V (2011) Production, recovery and applications of xanthan gum by Xanthomonas campestris. J Food Eng 106:1–12CrossRefGoogle Scholar
  18. 18.
    Wang L, Schiraldi DA, Sánchez-Soto M (2014) Foamlike xanthan gum/clay aerogel composites and tailoring properties by blending with agar. Ind Eng Chem Res 53:7680–7687CrossRefGoogle Scholar
  19. 19.
    Hamcerencu M, Desbrieres J, Popa M, Riess G (2009) Stimuli-sensitive xanthan derivatives/N-isopropylacrylamide hydrogels: influence of cross-linking agent on interpenetrating polymer network properties. Biomacromolecules 10:1911–1922CrossRefGoogle Scholar
  20. 20.
    Ghorai S, Sarkar A, Raoufi M, Panda AB, Schönherr H, Pal S (2014) Enhanced removal of methylene blue and methyl violet dyes from aqueous solution using a nanocomposite of hydrolyzed polyacrylamide grafted xanthan gum and incorporated nanosilica. ACS Appl Mater Interfaces 6:4766–4777CrossRefGoogle Scholar
  21. 21.
    Del Agua I, Marina S, Pitsalidis C, Mantione D, Ferro M, Iandolo D, Sanchez-Sanchez A, Malliaras GG, Owens RIM, Mecerreyes D (2018) Conducting polymer scaffolds based on poly (3, 4-ethylenedioxythiophene) and xanthan gum for live-cell monitoring. ACS Omega 3:7424–7431CrossRefGoogle Scholar
  22. 22.
    Silva-Medeiros FV, Vernasqui LG, Valderrama P (2016) Xanthan gum as a novel flocculant aid employed in drinking water treatment. Braz J Food Res 7:52–65CrossRefGoogle Scholar
  23. 23.
    Andrew T (1979) Application of xanthan gum in food and related products. Extracell Microb Polysaccharide 18:231–241Google Scholar
  24. 24.
    Zhang Y, Tian J, Li H, Wang L, Qin X, Asiri AM, Al-Youbi AO, Sun X (2012) Biomolecule-assisted, environmentally friendly, one-pot synthesis of CuS/reduced graphene oxide nanocomposites with enhanced photocatalytic performance. Langmuir 28:12893–12900CrossRefGoogle Scholar
  25. 25.
    Dutta S, Chatterjee S, Mukherjee I, Saha R, Singh BP (2017) Fabrication of ZnS hollow spheres and RGO-ZnS nanocomposite using cysteamine as novel sulfur source: photocatalytic performance on industrial dyes and effluent. Ind Eng Chem Res 56:4768–4778CrossRefGoogle Scholar
  26. 26.
    Zhang J, Feng H, Yang J, Qin Q, Fan H, Wei C, Zheng W (2015) Solvothermal synthesis of three-dimensional hierarchical CuS microspheres from a cu-based ionic liquid precursor for high-performance asymmetric supercapacitors. ACS Appl Mater Interfaces 7:21735–21744CrossRefGoogle Scholar
  27. 27.
    Wang P, Gao Y, Li P, Zhang X, Niu H, Zheng Z (2016) Doping Zn2+ in CuS Nanoflowers into chemically homogeneous Zn0. 49Cu0. 50S1. 01 Superlattice crystal structure as high-efficiency n-type photoelectric semiconductors. ACS Appl Mater Interfaces 8:15820–15827CrossRefGoogle Scholar
  28. 28.
    Li J, Yan D, Lu T, Qin W, Yao Y, Pan L (2017) Significantly improved sodium-ion storage performance of CuS nanosheets anchored into reduced graphene oxide with ether-based electrolyte. ACS Appl Mater Interfaces 9:2309–2316CrossRefGoogle Scholar
  29. 29.
    Kim WB, Lee SH, Cho M, Lee Y (2017) Facile and cost-effective CuS dendrite electrode for non-enzymatic glucose sensor. Sensors Actuators B Chem 249:161–167CrossRefGoogle Scholar
  30. 30.
    Zhao Y, Fan L, Zhang Y, Zhao H, Li X, Li Y, Wen L, Yan Z, Huo Z (2015) Hyper-branched cu@Cu2O coaxial nanowires mesh electrode for ultra-sensitive glucose detection. ACS Appl Mater Interfaces 7:16802–16812CrossRefGoogle Scholar
  31. 31.
    Fang B, Gu A, Wang G, Wang W, Feng Y, Zhang C, Zhang X (2009) Silver oxide nanowalls grown on cu substrate as an enzymeless glucose sensor. ACS Appl Mater Interfaces 1:2829–2834CrossRefGoogle Scholar
  32. 32.
    Yan X, Gu Y, Li C, Zheng B, Li Y, Zhang T, Zhang Z, Yang M (2018) A non-enzymatic glucose sensor based on the CuS nanoflakes–reduced graphene oxide nanocomposite. Anal Methods 10:381–388CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemical Engineering and BiotechnologyNational Taipei University of TechnologyTaipeiTaiwan, Republic of China
  2. 2.Institute of Organic and Polymeric Materials and Research and Development Center for Smart Textile TechnologyNational Taipei University of TechnologyTaipeiTaiwan, Republic of China

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