Microchimica Acta

, 187:15 | Cite as

Multiplexed fluorometric determination for three microRNAs in acute myocardial infarction by using duplex-specific nuclease and MoS2 nanosheets

  • Xue Zhu
  • Ke Wang
  • Yan Jin
  • Shuya Wang
  • Xiaoxiao Liu
  • Haohao Liu
  • Peiling Zhou
  • Chengjian YangEmail author
  • Zhijun HanEmail author
Original Paper


Circulating microRNAs are of diagnostic value for acute myocardial infarction (AMI). This study describes a fluorometric assay for multiplexed detection of the AMI biomarkers microRNA-499, microRNA-133a and microRNA-1. The assay involves the following two steps: (a) duplex-specific nuclease (DSN)-mediated signal amplification using aptamer-based sensor; (b) MoS2 nanosheets-based multiplexed fluorometric signal detection, and fluorometric signals have excitation/emission maxima at 492/518 nm for microRNA-499, 565/580 nm for microRNA-133a and 649/663 nm for microRNA-1. The assay has detection limits of around 100 fM for all three microRNAs. The assay is highly specific and rapid. It demonstrates that the expressions of microRNA-499, microRNA-133a and microRNA-1 are significantly higher in AMI patients. The ROC curves allow a clear distinction to be made between AMI group and non-AMI group.

Graphical abstract

Schematic representation of a multiplexed fluorometric method based on duplex-specific nuclease (DSN)-mediated signal amplification and MoS2 nanosheets-based fluorometric signal detection (DSN-MoS2) for rapid, sensitive and specific detection of microRNAs in AMI.


Acute myocardial infarction MicroRNA-499 MicroRNA-133a MicroRNA-1 Duplex-specific nuclease-mediated signal amplification MoS2 nanosheets-based fluorometric signal detection Quantitative real time-PCR 


Author contribution statement

Chengjian Yang and Zhijun Han design experiments; Yan Jin, Shuya Wang, Xiaoxiao Liu and Haohao Liu carry out experiments; Xue Zhu, Ke Wang and Peiling Zhou analyze experimental results; Xue Zhu and Ke Wang write the manuscript.

Funding information

This work was supported by grant from Natural Science Foundation of Jiangsu Province (No. BK20171147), Jiangsu Young Medical Talents (QNRC2016167), Six talent peaks project in Jiangsu Province (WSW-047), The Science and Technology Projects of Wuxi City (WX18IVJN016) and The Major Science and Technology Projects of Wuxi City (Z201804, MS201653).

Compliance with ethical standards

Declarations of interest

The authors declare that there is no conflict of interest regarding the publication of this paper.

Supplementary material

604_2019_3896_MOESM1_ESM.docx (72 kb)
ESM 1 (DOCX 72 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Xue Zhu
    • 1
  • Ke Wang
    • 1
  • Yan Jin
    • 2
  • Shuya Wang
    • 2
  • Xiaoxiao Liu
    • 2
  • Haohao Liu
    • 3
  • Peiling Zhou
    • 2
  • Chengjian Yang
    • 2
    Email author
  • Zhijun Han
    • 3
    Email author
  1. 1.Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear MedicineJiangsu Institute of Nuclear MedicineWuxiPeople’s Republic of China
  2. 2.Department of CardiologyThe Affiliated Wuxi NO.2 People’s Hospital of Nanjing Medical UniversityWuxiPeople’s Republic of China
  3. 3.Department of Laboratory MedicineThe Affiliated Wuxi NO.2 People’s Hospital of Nanjing Medical UniversityWuxiPeople’s Republic of China

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