Copper(II) 1,4-naphthalenedicarboxylate on copper foam nanowire arrays for electrochemical immunosensing of the prostate specific antigen
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Nanowires of copper(II)-based metal-organic frameworks (Cu-MOFs) of type Cu(II)(1,4-naphthalenedicarboxylic acid) (1,4-NDC) were deposited on the surface of a copper foam by immersion of Cu(OH)2 nanowires in a solution of 1,4-NDC. An electrochemical immunosensor for the prostate specific antigen (PSA) is obtained by using the nanowire arrays as a redox signal probe. The signal is generated by the conversion of Cu(I) and Cu(II) of Cu-MOFs nanowires. Cu(1,4-NDC) nanowires contain many uncoordinated carboxyl groups which can bind to the amino groups of the PSA antibody. When PSA antibody binds to PSA antigen during an immune response, the current signal will decrease due to the electrical insulation of PSA antigen. The decrease of current is directly proportional to the increase of PSA concentration. The immunosensor, best operated at a voltage of typically −0.08 V (vs. Ag/AgCl), has a low limit of detection (4.4 fg·mL−1) and a wide linear range (0.1 pg·mL−1 to 20 ng·mL−1). This meets the demands of clinical diagnosis (with values <4 ng·mL−1) in serum. The method was applied to the determination of PSA in spiked serum.
KeywordsMetal organic frameworks Nanomaterial Square wave voltammetry Electrochemical immunosensor Core-shell structure
This work was supported by the National Natural Science Foundation of China (No.21705103), the Applied Basic Research Project of Shanxi Province (No. 201801D221392), the Science and Technology Innovation Projects of Universities in Shanxi Province, the Graduate Education Innovation Project of Shanxi Province (2018SY057), Collaborative Innovation Center for Shanxi Advanced Permanent Materials and Technology, and the 1331 Engineering of Shanxi Province.
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Conflict of interest
There are no conflicts to declare.
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