Microchimica Acta

, 186:763 | Cite as

Whey peptide-encapsulated silver nanoparticles as a colorimetric and spectrophotometric probe for palladium(II)

  • Gajanan Ghodake
  • Surendra Shinde
  • Rijuta Ganesh Saratale
  • Avinash Kadam
  • Ganesh Dattatraya Saratale
  • Rahul Patel
  • Ashok Kumar
  • Sunil Kumar
  • Dae-Young KimEmail author
Original Paper


Silver nanoparticles (AgNPs) coated with whey peptides are shown to be a useful optical nanoprobe for the highly sensitive determination of Pd(II). The peptidic surface of the AgNPs works as a molecular receptor for the rapid detection of Pd(II) via a color change from dark yellow to orange/red along with a spectral red-shift with a gap about 120 nm. The effect is caused by the formation of a coordination complex between Pd(II) and the peptide ligands. This results in the aggregation of AgNPs and an absorbance spectral shift from 410 to 530 nm. The absorbance response is linear in the range 0.1 to 1.3 μM Pd(II) with a low detection limit of 115 nM. The nanoprobe responds within a few minutes and is not interfered by other metal ions except for Mg(II). The probe potentially can be applied to the determination of Pd(II) contamination in the products of Pd(II)−catalyzed organic reactions and in pharmaceutical settings.

Graphical abstract

Schematic representation of the nanoprobe for Pd(II). (a) Synthesis of whey peptide-coated silver nanoparticles (AgNPs), (b) the nanoprobe design for Pd(II) detection, (c) HR-TEM imaging and elemental mapping, (d) quantitative determination of Pd(II) (Inset shows colorimetric results).


Whey waste Optical probe Metal ions Molecular receptor Absorbance probe Whey proteins Coordination chemistry HR-TEM EDS 



This work was supported by the Dongguk University Research Fund of 2018-2020.

Compliance with ethical standards

Conflict of interest

The authors confirm that there is no conflict of interest to declare.

Supplementary material

604_2019_3877_MOESM1_ESM.docx (1.5 mb)
ESM 1 (DOCX 1560 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Gajanan Ghodake
    • 1
  • Surendra Shinde
    • 1
  • Rijuta Ganesh Saratale
    • 2
  • Avinash Kadam
    • 2
  • Ganesh Dattatraya Saratale
    • 3
  • Rahul Patel
    • 3
  • Ashok Kumar
    • 4
  • Sunil Kumar
    • 5
  • Dae-Young Kim
    • 1
    Email author
  1. 1.Department Biological and Environmental Science, College of Life Science and BiotechnologyDongguk University-SeoulGoyang-siRepublic of Korea
  2. 2.Research Institute of Biotechnology and Medical Converged ScienceDongguk University-SeoulGoyang-siRepublic of Korea
  3. 3.Department of Food Science and BiotechnologyDongguk University-SeoulGoyang-siRepublic of Korea
  4. 4.Department of Biotechnology and BioinformaticsJaypee University of Information TechnologySolanIndia
  5. 5.Quantum-functional Semiconductor Research CenterDongguk University-SeoulSeoulRepublic of Korea

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