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Microchimica Acta

, 186:687 | Cite as

Dual-probe (colorimetric and fluorometric) detection of ferritin using antibody-modified gold@carbon dot nanoconjugates

  • Eepsita Priyadarshini
  • Kamla Rawat
  • Himadri B. Bohidar
  • Paulraj RajamaniEmail author
Original Paper
  • 181 Downloads

Abstract

A dual-mode assay is described for immunological determination of the anemia biomarker ferritin. It is based on the use of a gold@carbon dot (Au@CD) nanoconjugate as a colorimetric and fluorescent probe. Au@CD is hydrophilic, easily surface modified and stable in aqueous solution. The Au@CD have a red color with blue-green fluorescence and were modified with antibody against ferritin. This allows bi-modal detection of ferritin. Assays can be performed in phosphate buffer and were also analyzed in (Bovine Serum Albumin) BSA and (Fetal Bovine Serum) FBS. Detection is based on antigen-antibody interaction underlying the classical sandwich model. Response to ferritin can be detected by spectrophotometry (at 570 nm) or fluorescence (at excitation/emission maxima of 354/454 nm). Under optimal conditions, the assay has a linear response in the 1 to 120 ngmL−1 ferritin concentration range and detection limits of 20 ng (colorimetrically) and 64 ng (fluorometrically).

Graphical abstract

Schematic representation of the function of the designed nanoprobe. The Au@CD nanoconjugates are functionalized with ferritin antibody in the initial step which specifically interacts with ferritin molecules leading to aggregation and subsequent changes in the optical and fluorescence signals.

Keywords

Bi-modal detection Antigen-antibody Immunoassay Anemia Nanoprobe Diagnostic sensor Binding efficiency Dynamic light scattering Aggregation Quenching 

Notes

Acknowledgments

EP is thankful to DST-SERB for National Postdoctoral Fellowship under the grant number PDF/2017/000024. The authors thank the Advanced Instrument Research Facility of the University for Analytical Characterization.

Compliance with ethical standards

Conflict of interest

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3802_MOESM1_ESM.docx (305 kb)
ESM 1 (DOCX 305 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Eepsita Priyadarshini
    • 1
  • Kamla Rawat
    • 2
  • Himadri B. Bohidar
    • 3
    • 4
  • Paulraj Rajamani
    • 1
    Email author
  1. 1.School of Environmental SciencesJawaharlal Nehru UniversityNew DelhiIndia
  2. 2.Department of Chemistry, School of Chemical and Life SciencesJamia HamdardNew DelhiIndia
  3. 3.School of Physical SciencesJawaharlal Nehru UniversityNew DelhiIndia
  4. 4.Special Centre for Nano SciencesJawaharlal Nehru UniversityNew DelhiIndia

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