Microchimica Acta

, 186:638 | Cite as

Aptamer-mediated N/Ce-doped carbon dots as a fluorescent and resonance Rayleigh scattering dual mode probe for arsenic(III)

  • Zhihao Zhang
  • Jiao Li
  • Xiyin Wang
  • Aihui LiangEmail author
  • Zhiliang JiangEmail author
Original Paper


Carbon dots doped with N/Ce, N/Eu and N/Tb were prepared by a microwave based hydrothermal technique. The fluorescence of the N/Ce co-doped carbon dots (CDN/Ce) is strongest. They have excitation/emission maxima at 340/441 nm. CDN/Ce was characterized by scanning electron microscopy, infrared and fluorescence spectroscopy. On addition of the nucleic acid aptamer (Apt) against arsenic(III) in pH 7 solution, the blue fluorescence of the doped carbon dots is partially quenched due to electrostatic interaction. On addition of As(III), it will bind to the aptamer, and the carbon dots are released. Hence, fluorescence becomes gradually restored. In addition, the resonance Rayleigh scattering signal (measured at 340 nm) is reduced. This dual-mode assay works in the 0.5–5.8 μg·L−1 As(III) concentration range and has a 0.2 μg·L−1 detection limit.

Graphical abstract

Schematic representation of fluorometric and resonance Rayleigh scattering dual mode analysis of As3+ by using coupled Apt and CDN/Ce probes. Apt: Aptamer. CD: Carbon dot. Flu: Fluorescence. RRS: Resonance Rayleigh scattering.


Rare earth-doped carbon dots Fluorescence As3 +  Single-stranded DNA Dual mode analysis 



This work was supported by the National Natural Science Foundation of China (No. 21667006, 21767004).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3764_MOESM1_ESM.doc (46.9 mb)
ESM 1 (DOC 46.8 mb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology for Science and Education Combined with Science and Technology Innovation BaseGuilinChina

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