Microchimica Acta

, 186:652 | Cite as

Non-conjugated polymer carbon dots for fluorometric determination of metronidazole

  • Shuliang Yang
  • Long Wang
  • Ling Zuo
  • Chun Zhao
  • Huiyu LiEmail author
  • Lan DingEmail author
Original Paper


Non-conjugated polymer carbon dots (PCDs) with a 9% fluorescence quantum yield were synthesized by a pyrolytic method using polyethyleneimine as the sole precursor. The PCDs have an average size about 2.1 nm and a blue fluorescence, with excitation/emission maxima at 380/457 nm, that is quenched by the drug metronidazole. The method has a linear response in the 0.06–15 μg mL−1 metronidazole concentration range and a 20 ng mL−1 detection limit. Milk samples were spiked at two levels (0.6 and 5.0 μg mL−1), and the recoveries of metronidazole are in the range of 96.7–102.2%.

Graphical abstract

Schematic representation of preparation of non-conjugated polymer carbon dots (PCDs) and detection of metronidazole. Metronidazole with negative charge is easy to produce electrostatic interaction with polyethyleneimine chain with positive charge, which leads to PCDs fluorescence quenching, so as to realize metronidazole detection.


Fluorescent material Fluorescent probe Inner-filter effect Polyethyleneimine Antibiotics Milk Metronidazole 



This work received the support of the Development Program of the Ministry of Science and Technology of Jilin Province, China (Grant number 20180201011GX).

Author contributions

The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript. ‡These authors contributed equally.

Compliance with ethical standards

The author(s) declares that they have no competing interests.

Supplementary material

604_2019_3746_MOESM1_ESM.docx (317 kb)
ESM 1 (DOCX 317 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of ChemistryJilin UniversityChangchunChina
  2. 2.Shenyang Academy of Environmental Sciences, Liaoning Provincial Key Laboratory for Urban EcologyShenyangChina
  3. 3.Department of Ophthalmologythe Second Hospital of Jilin UniversityChangchunChina
  4. 4.State Key Laboratory on Integrated Optoelectronics, College of Electronic Science & EngineeringJilin UniversityChangchunChina

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