Microchimica Acta

, 186:625 | Cite as

Fluorometric determination of sulfadiazine by using molecularly imprinted poly(methyl methacrylate) nanobeads doped with manganese(II)-doped ZnS quantum dots

  • Zhikun Gao
  • Yu Luan
  • Yi Lu
  • Zhiping Zhou
  • Tianshu Liu
  • Bolun Li
  • Zhifeng Qiu
  • Wenming YangEmail author
Original Paper


The surface of poly(methyl methacrylate) nanospheres (PMMA-NSs) was molecularly imprinted with sulfadiazine by a surface imprinting method. Simultaneously, Mn(II)-doped ZnS quantum dots were incorporated into the imprinted PMMA-NSs. The morphology of the fluorescent nanoprobe was characterized by transmission electron microscopy which revealed good spheroidal core-shell structure and a homogeneous distribution of the QDs. Following binding of sulfadiazine, fluorescence (best measured at excitation/emission maxima of 335/592 nm) is increasingly quenched. The detection range is 5–40 μmol·L−1 of sulfadiazine, and the detection limit is 0.24 μmol·L−1. The fluorescence quenching mechanism is discussed, and a photo-induced electron transfer process is shown to account for quenching. The fluorescent probe was applied to the determination of sulfadiazine in spiked tap water with recoveries and RSDs of 96.6–100.2% and 2.7–3.9%, respectively. The detection of sulfadiazine in spiked lake water exhibited the recoveries and RSDs with 99.3–104.8% and 1.8–4.2%, respectively.

Graphical abstract

Schematic presentation of synthesis of PMMA-Ns, Mn-doped ZnS QDs, MQPs, and the elution diagram of SD from MQPs, and the relative reagents including: sodium dodecyl benzene sulfonate(SDBS), (3-aminopropyl)triethoxysilane(APTES), 3-mercaptopropionic acid (MPA), tetraethylorthosilicate(TEOS)and sulfadiazine(SD), and nanoparticles including: polymer(methyl methacrylate) nanospheres(PMMANs), MIPs@QDs@PMMANs(MQPs) and carbon quantum dots(CQDs).


Molecular imprinting polymers Sulfadiazine detection Mn-doped ZnS quantum dots Absorbability Quenching mechanism 



This work was financially supported by the National Natural Science Foundation of China (Grant No. 21677064), Zhenjiang Natural Science Foundation of China (Grant Nos. SH2017046, SH2017048 and SH2017055), Scientific Research Foundation of Jiangsu University (Grant No. 17A008).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3721_MOESM1_ESM.docx (300 kb)
ESM 1 The support materials include: The materials and apparatus; the synthesis of Mn-doped ZnS QDs and PMMA-Ns; The diagrammatic drawing of PMMA-Ns preparation; The XRD spectra of PMMA-ns, Mn-doped ZnS QDs, MQPs and NQPs; The fluorescence intensity of MQPs and NQPs. The fluorescence intensity stability investigation of MQPs; The pH optimization; The structures of SD and similar molecules; The uncertainty research; the absorbability research; The UV-vis of SD and fluorescence intensity of MPQs; The UV-vis of SD, MQPs and mixture of SD and MQPs (DOCX 302 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Zhikun Gao
    • 1
  • Yu Luan
    • 2
  • Yi Lu
    • 3
  • Zhiping Zhou
    • 1
  • Tianshu Liu
    • 3
  • Bolun Li
    • 1
  • Zhifeng Qiu
    • 1
  • Wenming Yang
    • 1
    Email author
  1. 1.Institute of Polymer Materials, School of Materials Science and EngineeringJiangsu UniversityJiangsu ProvinceChina
  2. 2.Zhenjiang Food and Drug Supervision and Inspection CenterZhenjiangChina
  3. 3.Entry-Exit Inspection Quarantine BureauZhenjiangChina

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