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Microchimica Acta

, 186:637 | Cite as

A highly sensitive tetracycline sensor based on a combination of magnetic molecularly imprinted polymer nanoparticles and surface plasmon resonance detection

  • Wanru Gao
  • Pao Li
  • Si Qin
  • Zhao Huang
  • Yanan Cao
  • Xia LiuEmail author
Original Paper
  • 126 Downloads

Abstract

A method is reported for in-situ detection of the antibiotic tetracycline (TC). It is based on a combination of extraction of TC by magnetic molecularly imprinted polymers nanoparticles (MMIPs NPs) and detection by surface plasmon resonance (SPR). The TC-captured MMIPs NPs were flowed over the surface of the SPR chip that was modified with mercaptoethylamine. The SPR signal undergoes a strong increase through the use of MMIPs NPs. It increases linearly in the 5.0–100 pg·mL−1 TC concentration range, and the detection limit is as low as 1.0 pg·mL−1 (at S/N = 3). This method shows selectivity to TC compared with structurally analogues. In order to demonstrate the power of the method, it was applied to the analysis of milk spiked with TC. These results were validated by comparing them to those of an enzyme-linked immunoassay. The average recovery is in the range of 95.7–104.6%.

Graphical abstract

Schematic representation of a surface plasmon resonance assay for the sensitive determination of tetracycline in milk using the magnetic molecularly imprinted polymers nanoparticles that can extract tetracycline and amplify the SPR signal.

Keywords

Surface plasmon resonance Magnetic molecularly imprinted polymers nanoparticles Tetracycline Assay Specific recognition 

Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No.31671931; 31601551) and “1515” talent cultivation plan of Hunan Agricultural University.

Compliance with ethical standards

The author(s) declare that they have no competing interests. This article does not contain any studies with human oranimal subjects. Informed consent was obtained from all individual participants include in the study.

Supplementary material

604_2019_3718_MOESM1_ESM.doc (3.7 mb)
ESM 1 (DOC 3784 kb)

References

  1. 1.
    Jing T, Wang Y, Dai Q, Xia HA, Niu JW, Hao QL, Mei SR, Zhou YK (2010) Preparation of mixed-templates molecularly imprinted polymers and investigation of the recognition ability for tetracycline antibiotics. Biosens Bioelectron 25(10):2218–2224.  https://doi.org/10.1016/j.bios.2010.02.023 CrossRefPubMedGoogle Scholar
  2. 2.
    Lv YK, Wang LM, Yang L, Zhao CX, Sun HW (2012) Synthesis and application of molecularly imprinted poly (methacrylic acid)-silica hybrid composite material for selective solid-phase extraction and high-performance liquid chromatography determination of oxytetracycline residues in milk. J Chromatogr A 1227:48–53.  https://doi.org/10.1016/j.chroma.2011.12.108 CrossRefPubMedGoogle Scholar
  3. 3.
    Gondová Z, Kožárová I, Poláková Z, Mad´arová M (2014) Comparison of four microbiological inhibition tests for the screening of antimicrobial residues in the tissues of food-producing animals. Ital J Anim Sci 13(4):728–734.  https://doi.org/10.4081/ijas.2014.3521 CrossRefGoogle Scholar
  4. 4.
    Shahbazi Y, Ahmadi F, Karami N (2015) Screening, determination and confirmation of tetracycline residues in chicken tissues using four-plate test, ELISA and HPLC-UV methods: comparison between correlation results. Food Agric Immunol 26(6):821–834.  https://doi.org/10.1080/09540105.2015.1036357 CrossRefGoogle Scholar
  5. 5.
    Chen YN, Kong DZ, Liu LQ, Song SS, Kuang H, Xu CL (2016) Development of an ELISA and immunochromatographic assay for tetracycline, oxytetracycline, and chlortetracycline residues in milk and honey based on the class-specific monoclonal antibody. Food Anal Methods 9(4):905–914.  https://doi.org/10.1007/s12161-015-0262-z CrossRefGoogle Scholar
  6. 6.
    Benvidi A, Tezerjani MD, Moshtaghiun SM, Mazloum-Ardakani M (2016) An aptasensor for tetracycline using a glassy carbon modified with nanosheets of graphene oxide. Microchim Acta 183(5):1797–1804.  https://doi.org/10.1007/s00604-016-1810-y CrossRefGoogle Scholar
  7. 7.
    Zhang XP, Zhang RR, Yang AJ, Wang Q, Kong RM, Qu FL (2017) Aptamer based photoelectrochemical determination of tetracycline using a spindle-like ZnO-CdS@Au nanocomposite. Microchim Acta 184(11):4367–4374.  https://doi.org/10.1007/s00604-017-2477-8 CrossRefGoogle Scholar
  8. 8.
    Shalaby AR, Salama NA, Abou-Raya SH, Emam WH, Mehaya FM (2011) Validation of HPLC method for determination of tetracycline residues in chicken meat and liver. Food Chem 124(4):1660–1666.  https://doi.org/10.1016/j.foodchem.2010.07.048 CrossRefGoogle Scholar
  9. 9.
    Ahmadi F, Shahbazi Y, Karami N (2015) Determination of tetracyclines in meat using two phases freezing extraction method and HPLC-DAD. Food Anal Methods 8(7):1883–1891.  https://doi.org/10.1007/s12161-014-0073-7 CrossRefGoogle Scholar
  10. 10.
    Zhu WX, Yang JZ, Wang ZX, Wang CJ, Liu YF, Zhang L (2016) Rapid determination of 88 veterinary drug residues in milk using automated TurborFlow online clean-up mode coupled to liquid chromatography-tandem mass spectrometry. Talanta 148:401–411.  https://doi.org/10.1016/j.talanta.2015.10.037 CrossRefPubMedGoogle Scholar
  11. 11.
    Gavilán RE, Nebot C, Miranda JM, Martín-Gómez Y, Vázquez-Belda B, Franco CM, Cepeda A (2016) Analysis of tetracyclines in medicated feed for food animal production by HPLC-MS/MS. Antibiot 5:1): 1–1):10.  https://doi.org/10.3390/antibiotics5010001 CrossRefGoogle Scholar
  12. 12.
    Dumont V, Huet AC, Traynor I, Elliott C, Delahaut P (2006) A surface plasmon resonance biosensor assay for the simultaneous determination of thiamphenicol, florefenicol, florefenicol amine and chloramphenicol residues in shrimps. Anal Chim Acta 567(2):179–183.  https://doi.org/10.1016/j.aca.2006.03.028 CrossRefGoogle Scholar
  13. 13.
    Kim S, Lee HJ (2017) Gold nanostar enhanced surface plasmon resonance detection of an antibiotic at attomolar concentrations via an aptamer-antibody sandwich assay. Anal Chem 89(12):6624–6630.  https://doi.org/10.1021/acs.analchem.7b00779 CrossRefPubMedGoogle Scholar
  14. 14.
    Wang S, Dong YY, Liang XG (2018) Development of a SPR aptasensor containing oriented aptamer for direct capture and detection of tetracycline in multiple honey samples. Biosens Bioelectron 109:1–7.  https://doi.org/10.1016/j.bios.2018.02.051 CrossRefPubMedGoogle Scholar
  15. 15.
    Wang JL, Munir A, Zhu ZZ, Zhou HS (2010) Magnetic nanoparticle enhanced surface plasmon resonance sensing and its application for the ultrasensitive detection of magnetic nanoparticle-enriched small molecules. Anal Chem 82(16):6782–6789.  https://doi.org/10.1021/ac100812c CrossRefPubMedGoogle Scholar
  16. 16.
    Garibo D, Campbell K, Casanova A, de la lglesia P, Fernández-Tejedor M, Diogène J, Elliott CT, Campàs M (2014) SPR immunosensor for the detection of okadaic acid in mussels using magnetic particles as antibody carriers. Sensor Actuat B-Chem 190:822–828.  https://doi.org/10.1016/j.snb.2013.09.037 CrossRefGoogle Scholar
  17. 17.
    Jia YT, Peng Y, Bai JL, Zhang XH, Cui YG, Ning BA, Cui JS, Gao ZX (2018) Magnetic nanoparticle enhanced surface plasmon resonance sensor for estradiol analysis. Sensor Actuat B-Chem 254:629–635.  https://doi.org/10.1016/j.snb.2017.07.061 CrossRefGoogle Scholar
  18. 18.
    Vlatakis G, Andersson LI, Müller R, Mosbach K (1993) Drug assay using antibody mimics made by molecular imprinting. Nature 361(6413):645–647.  https://doi.org/10.1038/361645a0 CrossRefPubMedGoogle Scholar
  19. 19.
    Xiao DL, Jiang Y, Bi YP (2018) Molecularly imprinted polymers for the detection of illegal drugs and additives: a review. Microchim Acta 185:247.  https://doi.org/10.1007/s00604-018-2735-4 CrossRefGoogle Scholar
  20. 20.
    Hu MH, Huang PC, Suo LL, Wu FY (2018) Polydopamine-based molecularly imprinting polymers on magnetic nanoparticles for recognition and enrichment of ochratoxins prior to their determination by HPLC. Microchim Acta 185:300.  https://doi.org/10.1007/s00604-018-2826-2 CrossRefGoogle Scholar
  21. 21.
    Wen TT, Zhu WY, Xue C, Wu JH, Han Q, Wang X, Zhou XM, Jiang HJ (2014) Novel electrochemical sensing platform based on magnetic field-induced self-assembly of Fe3O4@polyaniline nanoparticles for clinical detection of creatinine. Biosens Bioelectron 56:180–185.  https://doi.org/10.1016/j.bios.2014.01.013 CrossRefPubMedGoogle Scholar
  22. 22.
    Han Q, Wang X, Yang ZY, Zhu WY, Zhou XM, Jiang HJ (2014) Fe3O4@rGO doped molecularly imprinted polymer membrane based on magnetic field directed self-assembly for the determination of amaranth. Talanta 123:101–108.  https://doi.org/10.1016/j.talanta.2014.01.060 CrossRefPubMedGoogle Scholar
  23. 23.
    Hassan AHA, Moura SL, Ali FHM, Moselhy WA, Sotomayor MDT, Pividori MI (2018) Electrochemical sensing of methyl parathion on magnetic molecularly imprinted polymer. Biosens Bioelectron 118:181–187.  https://doi.org/10.1016/j.bios.2018.06.052 CrossRefPubMedGoogle Scholar
  24. 24.
    Wang JL, Zhu ZZ, Munir A, Zhou H (2011) Fe3O4 nanoparticles-enhanced SPR sensing for ultrasensitive sandwich bio-assay. Talanta 84(3):783–788.  https://doi.org/10.1016/j.talanta.2011.02.020 CrossRefPubMedGoogle Scholar
  25. 25.
    Lou ZC, Zhang YN, Zhang XH, Zhou ZW, Hu XD, Zhang HQ (2015) A facile approach to monodisperse Au nanoparticles on Fe3O4 nanostructures with surface plasmon resonance amplification. J Nanosci Nanotechnol 15(3):2371–2378.  https://doi.org/10.1166/jnn.2015.9095 CrossRefPubMedGoogle Scholar
  26. 26.
    Yao GH, Liang RP, Huang CF, Wang Y, Qiu JD (2013) Surface plasmon resonance sensor based on magnetic molecularly imprinted polymers amplification for pesticide recognition. Anal Chem 85(24):11944–11951.  https://doi.org/10.1021/ac402848x CrossRefPubMedGoogle Scholar
  27. 27.
    Zhou CJ, Rong PF, Zhang WJ, Zhou JD, Zhang QL, Wang W, Zou BS (2010) Fulvic acid coated iron oxide nanoparticles for magnetic resonance imaging contrast agent. Funct Mater Lett 3(3):197–200.  https://doi.org/10.1142/S179360471000124X CrossRefGoogle Scholar
  28. 28.
    Singh D, Gautam RK, Kumar R, Shukla BK, Shankar V, Krishna V (2014) Citric acid coated magnetic nanoparticles: synthesis, characterization and application in removal of Cd(II) ions from aqueous solution. J Water Process Eng 4:233–241.  https://doi.org/10.1016/j.jwpe.2014.10.005 CrossRefGoogle Scholar
  29. 29.
    Zhang XP, Chen LG, Xu Y, Wang H, Zeng QL, Zhao Q, Ren NQ, Ding L (2010) Determination of beta-lactam antibiotics in milk based on magnetic molecularly imprinted polymer extraction coupled with liquid chromatography-tandem mass spectrometry. J Chromatogr B 878(32):3421–3426.  https://doi.org/10.1016/j.jchromb.2010.10.030 CrossRefGoogle Scholar
  30. 30.
    Ahmadi F, Rezaei H, Tahvilian R (2012) Computational-aided design of molecularly imprinted polymer for selective extraction of methadone from plasma and saliva and determination by gas chromatography. J Chromatogr A 1270:9–19.  https://doi.org/10.1016/j.chroma.2012.10.038 CrossRefPubMedGoogle Scholar
  31. 31.
    Ahmadi F, Yawari E, Nikbakht M (2014) Computational design of an enantioselective molecular imprinted polymer for the solid phase extraction of S-warfarin from plasma. J Chromatogr A 1338:9–16.  https://doi.org/10.1016/j.chroma.2014.02.055 CrossRefPubMedGoogle Scholar
  32. 32.
    Ramezani M, Danesh NM, Lavaee P, Abnous K, Taghdisi SM (2015) A novel colorimetric triple-helix molecular switch aptasensor for ultrasensitive detection of tetracycline. Biosens Bioelectron 70:181–187.  https://doi.org/10.1016/j.bios.2015.03.040 CrossRefPubMedGoogle Scholar
  33. 33.
    Hou WJ, Shi ZQ, Guo YM, Sun X, Wang XY (2017) An interdigital array microelectrode aptasensor based on multi-walled carbon nanotubes for detection of tetracycline. Bioprocess Biosyst Eng 40(9):1419–1425.  https://doi.org/10.1007/s00449-017-1799-6 CrossRefPubMedGoogle Scholar
  34. 34.
    Qi MY, Tu CY, Dai YY, Wang WP, Wang AJ, Chen JR (2018) A simple colorimetric analytical assay using gold nanoparticles for specific detection of tetracycline in environmental water samples. Anal Methods 10(27):3402–3407.  https://doi.org/10.1039/C8AY00713F CrossRefGoogle Scholar
  35. 35.
    He W, Sun XY, Weng WT, Liu B (2018) Fluorescence enhancement of carbon dots by graphene for highly sensitive detection of tetracycline hydrochloride. RSC Adv 8(46):26224–26229.  https://doi.org/10.1039/C8RA04581J CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Wanru Gao
    • 1
  • Pao Li
    • 1
  • Si Qin
    • 1
  • Zhao Huang
    • 1
  • Yanan Cao
    • 1
  • Xia Liu
    • 1
    Email author
  1. 1.Hunan Province Key Laboratory of Food Science and Biotechnology, Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, College of Food Science and TechnologyHunan Agricultural UniversityChangshaPeople’s Republic of China

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