Microchimica Acta

, 186:605 | Cite as

Determination of folic acid via its quenching effect on the fluorescence of MoS2 quantum dots

  • Yage PengEmail author
  • Wenfei Dong
  • Le Wan
  • Xiaosai Quan
Original Paper


Molybdenum disulfide quantum dots (MoS2 QDs) are used in a fluorometric method for the determination of folic acid (FA) based on fluorescence quenching. The MoS2 QDs synthesized by a hydrothermal method possess bright blue fluorescence (with excitation/emission maxima of 325/415 nm), quantum yield of 3.7%, and excellent storage stability in solution (30 days in the refrigerator). Their fluorescence is quenched by FA, and intensity decreases linearly in the 0.1 to 125 μM FA concentration range. The detection limit is 0.1 μM (at S/N = 3), and the relative standard deviation (for n = 5) is 2.8% for 25 μM concentrations of FA. Studies on the quenching mechanism suggest that the effect is due to static quenching. The FA in commercial FA tablets was successfully determined.

Graphical abstract

Schematic representation of the hydrothermal method for the preparation of molybdenum disulfide quantum dots (MoS2 QDs) with about 2.7 ± 0.5 nm diameter using Na2MoO4 and L-cysteine as Mo and S sources, and the fluorescence method for the determination of folic acid (FA) based on fluorescence quenching of MoS2 QDs.


MoS2 QDs Fluorescence analysis Quenching mechanism Folic acid 



This work was supported by the Scientific Research Projects of Ningxia Colleges and Universities (NGY2017043), the Discipline Project of Ningxia (No. NXYLXK2017A04), and the National Natural Science Foundation of China (21765017).

Author contributions

Yage Peng devised the experiments. Wenfei Dong performed the experiments. Wenfei Dong and Le Wan discussed to prepare MoS2 QDs. Wenfei Dong and Xiaosai Quan performed the HPLC experiment and the analysis of the HPLC data. Yage Peng and Wenfei Dong wrote the manuscript. All authors participated in discussions of the results.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3705_MOESM1_ESM.doc (3.9 mb)
ESM 1 (DOC 3990 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, National Demonstration Center for Experimental Chemistry Education, College of Chemistry and Chemical EngineeringNingxia UniversityYinchuanPeople’s Republic of China

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