Determination of folic acid via its quenching effect on the fluorescence of MoS2 quantum dots
- 178 Downloads
Molybdenum disulfide quantum dots (MoS2 QDs) are used in a fluorometric method for the determination of folic acid (FA) based on fluorescence quenching. The MoS2 QDs synthesized by a hydrothermal method possess bright blue fluorescence (with excitation/emission maxima of 325/415 nm), quantum yield of 3.7%, and excellent storage stability in solution (30 days in the refrigerator). Their fluorescence is quenched by FA, and intensity decreases linearly in the 0.1 to 125 μM FA concentration range. The detection limit is 0.1 μM (at S/N = 3), and the relative standard deviation (for n = 5) is 2.8% for 25 μM concentrations of FA. Studies on the quenching mechanism suggest that the effect is due to static quenching. The FA in commercial FA tablets was successfully determined.
KeywordsMoS2 QDs Fluorescence analysis Quenching mechanism Folic acid
This work was supported by the Scientific Research Projects of Ningxia Colleges and Universities (NGY2017043), the Discipline Project of Ningxia (No. NXYLXK2017A04), and the National Natural Science Foundation of China (21765017).
Yage Peng devised the experiments. Wenfei Dong performed the experiments. Wenfei Dong and Le Wan discussed to prepare MoS2 QDs. Wenfei Dong and Xiaosai Quan performed the HPLC experiment and the analysis of the HPLC data. Yage Peng and Wenfei Dong wrote the manuscript. All authors participated in discussions of the results.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 30.Xu Q, Liu Y, Su R, Cai L, Li B, Zhang Y, Zhang L, Wang Y, Wang Y, Li N, Gong X, Gu Z, Chen Y, Tan Y, Dong C, Sreeprasad TS (2016) Highly fluorescent Zn-doped carbon dots as Fenton reaction-based bio-sensors: an integrative experimental-theoretical consideration. Nanoscale 8:17919–17927CrossRefGoogle Scholar
- 32.Lakowicz JR (2006) Principles of fluorescence spectroscopy. 3rd ed. Springer, p 282Google Scholar