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Microchimica Acta

, 186:617 | Cite as

Modification of N,S co-doped graphene quantum dots with p-aminothiophenol-functionalized gold nanoparticles for molecular imprint-based voltammetric determination of the antiviral drug sofosbuvir

  • Ashraf M. Mahmoud
  • Mohamed M. El-WekilEmail author
  • Mater H. Mahnashi
  • Marwa F. B. Ali
  • Saad A. Alkahtani
Original Paper
  • 64 Downloads

Abstract

A molecularly imprinted polymer (MIP) was developed for the electrochemical determination of the antiviral drug sofosbuvir (SOF). The MIP was obtained by polymerization of p-aminothiophenol (p-ATP) on N,S co-doped graphene quantum dots (N,S@GQDs) in the presence of gold nanoparticles to form gold-sulfur covalent network. The presence of quantum dots improves the electron transfer rate, enhances surface activity and amplifies the signal. The nanocomposites were characterized by FTIR, TEM, EDX, and SEM. The electrochemical performance of the electrode was investigated by differential pulse voltammetry and cyclic voltammetry. The sensor uses hexacyanoferrate as the redox probe and is best operated at a potential of around 0.36 V vs. Ag/AgCl. It has a linear response over the concentration range of 1–400 nM SOF, with a detection limit of 0.36 nM. Other features include high selectivity, good reproducibility and temporal stability. The sensor was applied to the determination of SOF in spiked human plasma.

Graphical abstract

Novel sofosbuvir imprinted p-ATP polymer was synthesized by the aid of gold nanoparticles on N,S co-doped graphene quantum dots as a good conductive support. The imprinted polymer was used for detection of sofosbuvir in real samples by using the ferri/ferrocyanide redox probe.

Keywords

Electrochemical sensor Gold-sulfur covalent network Characterization techniques Selectivity Temporal stability Human plasma 

Notes

Acknowledgements

The authors thank the Deanship of Scientific Research, Najran University for funding the work; Project Code [NU/MID/16/032].

Compliance with ethical standards

The author(s) declare that they have no competing interest.

Ethical standards and informed consent

Collection of samples was from healthy volunteers at Assiut University Clinics. All experimental procedures and protocols were reviewed and approved by the ethics of Assiut University clinics, Assiut, Egypt. Informed consents were obtained from the human participants for this study.

Supplementary material

604_2019_3647_MOESM1_ESM.docx (690 kb)
ESM 1 (DOCX 690 KB)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Pharmaceutical Chemistry, College of PharmacyNajran UniversityNajranSaudi Arabia
  2. 2.Department of Pharmaceutical Analytical Chemistry, Faculty of PharmacyAssiut UniversityAssiutEgypt
  3. 3.Department of Clinical Pharmacy, College of PharmacyNajran UniversityNajranSaudi Arabia

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