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Microchimica Acta

, 186:358 | Cite as

Electrochemical recognition of tryptophan enantiomers using a multi-walled carbon nanotube@polydopamine composite loaded with copper(II)

  • Junjuan Qian
  • Yinhui YiEmail author
  • Depeng Zhang
  • Gangbing ZhuEmail author
Original Paper
  • 13 Downloads

Abstract

The work describes a voltammetric method for the recognition of tryptophan (Trp) enantiomers. A glassy carbon electrode (GCE) was modified with polydopamine-coated multiwalled carbon nanotubes and subsequently loaded with copper(II) ions. The morphology and structure of the material were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, Raman spectroscopy and electrochemical methods. The recognition of Trp enantiomers by the modified GCE was investigated by differential pulse voltammetry. Under optimum conditions, the sensor revealed a linear range from 1.0 to 100.0 μM with the limit of detection values of 0.15 μM and 0.20 μM for D-Trp and L-Trp, respectively. The recognition efficiency for the Trp enantiomers (with a chiral separation factor of 5.4 for L-Trp over D-Trp) is much higher than that of other electrodes. This is assumed to be due to the unique features of MWCNTs, PDA and Cu(II). After optimizing various experimental conditions, the method was successfully applied to chiral sensing of Trp isomers in a racemic mixture. The potential application to chiral separation of the amino acids phenylalanine and tyrosine was also evaluated, with a chiral separation factor of 2.14 and 1.33 for L−/D-phenylalanine and L−/D-tyrosine, respectively.

Graphical abstract

Schematic presentation of the synthesis of a multi-walled carbon nanotubes@polydopamine composite loaded with copper(II), and its application in electrochemical enantiorecognition of tryptophan enantiomers.

Keywords

Chiral recognition Chiral sensing Tryptophan Electrochemical sensors Chiral amino acids 

Notes

Acknowledgments

We acknowledge the support from the National Natural Science Foundation of China (21607061), the Natural Science Foundation of Jiangsu Province (BK20190543)the Opening Project of State Key Laboratory of Chemo/Biosensing and Chemometrics of Hunan University (2018019), the Priority Academic Program Development of Jiangsu Higher Education Institutions, Collaborative Innovation Center of Technology and Material of Water Treatment, and the Program of Young Backbone Teachers in Jiangsu University (2015).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3469_MOESM1_ESM.docx (532 kb)
ESM 1 (DOCX 531 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of the Environment and Safety EngineeringJiangsu UniversityZhenjiangPeople’s Republic of China

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