Gold-platinum nanoflowers as a label and as an enzyme mimic for use in highly sensitive lateral flow immunoassays: application to detection of rabbit IgG
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The authors describe the preparation of gold-platinum nanoflower (AuPt NFs) and show that they can be simultaneously used as a label and as an enzyme mimic in lateral flow immunoassays (LFIs). The AuPt NFs were prepared by growing Pt nanowires on the surface of gold nanoparticle. The assay involves the capture of target proteins (here: rabbit IgG as a model analyte) by the immobilized capture antibody, and by using AuPt NF-labeled secondary antibody. The AuPt NFs are thus captured by the test zone and produce a characteristic black band for visual detection of the antigen (IgG). The coloration of the test line can be further enhanced by addition of the chromogenic substrate 3-amino-9-ethyl-carbazole which is catalytically oxidized by the captured Pt nanowires on the AuPt NF and produce a red coloration. Quantitative results were obtained by reading the test line intensities with a portable strip reader. The LFI has a 5 pg mL-1 detection limit for IgG under optimized experimental conditions. This is 100 times lower than that of the conventional AuNP-based LFI. Conceivably, this assay has a wide scope in that it may be applied to numerous other targets for which appropriate antibodies are available.
KeywordsGold nanoparticles Platinum; enzyme mimic Nanoflower Lateral flow immunoassay
This research was supported by the National Institute of Health, Centers of Biomedical Research Excellence (NIH, COBRE, Grant number: P20 GM109024), the National Natural Science Foundation of China (Grant No. 31700735) and the National Natural Science Foundation of Anhui Province (Grant No: 1908085 MB54, 1808085QH264). Its contents are solely the responsibility of the authors, and do not necessarily represent the official views of the NIH. G. Liu acknowledges the support from the Wanjiang scholars in Anhui Province, China.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 11.Moussa A (2016) Electrovphoresis and western blot can detect the interaction of proteins with the pathogenic prion protein. J Chromatogr 7:3Google Scholar
- 18.Jarvis JN, Percival A, Bauman S, Pelfrey J, Meintjes G, Williams GN, Longley N, Harrison TS, Kozel TR (2011) Evaluation of a novel point-of-care cryptococcal antigen test on serum, plasma, and urine from patients with HIV-associated cryptococcal meningitis. Clin Infect Dis 53:1019–1023CrossRefPubMedPubMedCentralGoogle Scholar
- 19.Moreno ML, Cebolla A, Munoz-Suano A, Carrillo-Carrion C, Comino I, Pizarro A, Leon F, Rodriguez-Herrera A, Sousa C (2017) Detection of gluten immunogenic peptides in the urine of patients with coeliac disease reveals transgressions in the gluten-free diet and incomplete mucosal healing. Gut 66:250–257CrossRefPubMedGoogle Scholar
- 40.Loynachan CN, Thomas MR, Gray ER, Richards DA, Kim JY, Miller BS, Brookes JC, Agarwal S, Chudasama V, Mckendry RA, Stevens MM (2017) Platinum Nanocatalyst amplification: redefining the gold standard for lateral flow immunoassays with ultra-broad dynamic range. ACS Nano 12:279–288CrossRefPubMedPubMedCentralGoogle Scholar