Microchimica Acta

, 186:356 | Cite as

A versatile fluorometric aptasensing scheme based on the use of a hybrid material composed of polypyrrole nanoparticles and DNA-silver nanoclusters: application to the determination of adenosine, thrombin, or interferon-gamma

  • Jing Wang
  • Bingzhi Li
  • Qiaoyun Lu
  • Xiaoyun Li
  • Chenyuan Weng
  • Xiaoqiang Yan
  • Junli Hong
  • Xuemin ZhouEmail author
Original Paper


The authors describe a versatile aptasensing scheme based on the use of polypyrrole nanoparticles (PPyNPs) and DNA-silver nanoclusters (DNA-AgNCs) for multiple target detection. The DNA-AgNCs consist of two functional domains, viz. (a) a nucleation domain for attaching the metal core of the nanoclusters, and (b) a recognition domain which consists of a single-stranded aptamer. In the absence of analytes, the single-strand recognition domain will be absorbed onto the surface of the PPyNPs through π stacking and hydrophobic interactions. As a result, the red fluorescence of the DNA-AgNCs (with excitation/emission peaks at 535/625 nm) is quenched by the PPyNPs. On introducing the analytes, the DNA-AgNCs will bind them. This leads to the desorption of DNA-AgNCs and the recovery of the red fluorescence. Based on the above strategy, a versatile, sensitive and selective aptasensor was established for detection of adenosine, thrombin and interferon-gamma. The method was applied to the detection of the above targets in (spiked) serum samples and gave satisfactory results, with detection limit of 0.58 nM for IFN-γ, 0.39 nM for adenosine, and 2.2 nM for thrombin. The use of PPyNPs results in uniquely low non-specific absorption and in improved analytical results in case of real-sample analysis when compared to previously reported methods.

Graphical abstract

Schematic illustration of DNA-silver nanoclusters and polypyrrole nanoparticles in an aptasensor for detection of multiple targets.


Aptasensor DNA-silver nanoclusters Steric hindrance Polypyrrole nanoparticles Hybrid system Nanosensor Adenosine Thrombin Interferon-gamma Nucleic acid Fluorescence 



This work was supported by the National Natural Science Foundation of China (Nos. 81773681, 81572081 and 81273480). B.Z.L. acknowledges funding from Postgraduate Research & Practice Innovation Program of Jiangsu Province (JX22013405).

Compliance with ethical standards

The authors declare no competing financial or non-financial interest.

Supplementary material

604_2019_3459_MOESM1_ESM.doc (1.4 mb)
ESM 1 (DOC 1.44 mb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of PharmacyNanjing Medical, UniversityNanjingChina
  2. 2.Drug Clinical Trial InstitutionWuxi People’s Hospital, Nanjing Medical UniversityWuxiChina

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