Microchimica Acta

, 186:277 | Cite as

Ratiometric determination of hydrogen peroxide based on the size-dependent green and red fluorescence of CdTe quantum dots capped with 3-mercaptopropionic acid

  • Yongbo WangEmail author
  • Min Yang
  • Yingkun Ren
  • Jun Fan
Original Paper


A ratiometric fluorescent nanoprobe is described for the detection of H2O2. It is based on the use of a mixture of green-emitting CdTe quantum dots (GQDs) and red-emitting CdTe QDs (RQDs). The two kinds of QDs have different size and different fluorescence response towards H2O2. The ratio of the emission intensities at 606 and 510 nm (under 365 nm photoexcitation) can be used as the analytical information. Even without any chemical modification of the surface of the QDs, the probe display high sensitivity and selectivity for H2O2. The fluorescence of small QDs is more effectively quenched by H2O2. Stern-Volmer analysis showed both static and dynamic quenching to occur. The probe works well in the 10~125 μM H2O2 concentration range and has a 0.3 μM detection limit (3σ/slope).

Graphical abstract

Schematic presentation of the ratiometric fluorescent nanoprobe composed of green-emitting and red-emitting CdTe QDs. λ, I, and k are the emission wavelength, emission intensity, and quenching/enhancement coefficient, respectively. The subscript 0 and 1 present the green-emitting and red-emitting CdTe QDs, respectively.


Ratiometric fluorescent nanoprobe Binary QD systems Fluorescent detection Fluorescence intensity ratios Quenching efficiency Stern-Volmer plot Biomarkers Real samples Quenching mechanism 



This work was supported by the National Natural Science Foundation of China (Grant No. 21476183).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3390_MOESM1_ESM.docx (1.1 mb)
ESM 1 (DOCX 1075 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Food and Biological EngineeringShaanxi University of Science and TechnologyXi’anPeople’s Republic of China
  2. 2.School of Chemical EngineeringNorthwest UniversityXi’anPeople’s Republic of China

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