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Microchimica Acta

, 186:270 | Cite as

A glassy carbon electrode modified with molecularly imprinted poly(aniline boronic acid) coated onto carbon nanotubes for potentiometric sensing of sialic acid

  • Fuhui Huang
  • Bengao Zhu
  • Haochen Zhang
  • Yue Gao
  • Chunmei DingEmail author
  • Hong Tan
  • Jianshu LiEmail author
Original Paper

Abstract

A potentiometric sensor for sialic acid (SA) was developed based on molecular imprinting technique. The sensor was fabricated by modifying carbon nanotubes (CNT) and an SA-imprinted poly(aniline boronic acid) (PABA) film on a glassy carbon electrode (GCE). The detection strategy capitalizes on the change of electrochemical potential resulting from boronic acid-SA interaction. The imprinted PABA combines the functions of SA-binding boronic acid groups and the imprinting effect, thus endowing it with both chemical and sterical recognition capability. The imprint factor (IF, compared to a non-molecularly imprinted polymer) is 1.74. The sensor can well differentiate SA from its analogs at physiological pH values and has a linear potentiometric response (R2 = 0.998) in 80 μM to 8.2 mM SA concentrations range with a detection limit of 60 μM (at S/N = 3). The sensor was applied to the determination of SA in serum samples and gave recoveries between 93% and 105%.

Graphical abstract

Schematic presentation of the fabrication of a sialic acid (SA) imprinted poly(aniline boronic acid) (PABA)/CNT modified electrode. The electrode can well differentiate SA from its analogs at physiological pH and determine SA in human serum samples with satisfactory recoveries of 93%–105%.

Keywords

Sialic acid Poly(aniline boronic acid) Carbon nanotubes Physiological pH value Molecularly imprinted polymer Potentiometric response Electrochemical sensor 

Notes

Acknowledgements

The authors are grateful to the financial support by the National Natural Science Foundation of China (51503126 and 21534008) and the kind help of Ms. Fan Yang from Guanghan Chenglin Hospital.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3387_MOESM1_ESM.docx (15.9 mb)
ESM 1 (DOCX 16285 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Polymer Science and EngineeringSichuan UniversityChengduChina
  2. 2.State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengduChina

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