Microchimica Acta

, 186:287 | Cite as

Fluorometric determination of cardiac myoglobin based on energy transfer from a pyrene-labeled aptamer to graphene oxide

  • Dongkui Liu
  • Yanbo Zeng
  • Guobao Zhou
  • Xing Lu
  • Dongwei Miao
  • Yiwen YangEmail author
  • Yunyun Zhai
  • Jian Zhang
  • Zulei Zhang
  • Hailong Wang
  • Lei LiEmail author
Original Paper


The authors describe a fluorometric assay for cardiac myoglobin (Mb), a marker for myocardial infarction. An Mb-binding aptamer was labeled with pyrene and adsorbed on the surface of graphene oxide (GO) via noncovalent and reversible binding forces. This causes the fluorescence of pyrene (best measured at excitation/emission wavelengths of 275/376 nm) to be quenched. However, fluorescence is restored on addition of pyrene due to the strong affinity between Mb and aptamer which causes its separation from GO. Fluorescence increases linearly in the 5.6–450 pM Mb concentration range, and the lower detection limit is 3.9 pM (S/N = 3). The assay was applied to the determination of cardiac Mb in spiked serum, and satisfactory results were obtained.

Graphical abstract

Schematic presentation of the detection of Mb (cardiac myoglobin) by using a fluorometric method based on pyrene-modified anti-Mb aptamer and GO (graphene oxide) through fluorescence quenching and subsequent recovery.


Chemical modifications PMAMA probe FRET Fluorophore Fluorescent recovery 



The Authors acknowledge the National Natural Science Foundation of China (No. 21677060 and 51503079), the Public Welfare Research Project of Zhejiang Province (No. LGF18B050004), the Natural Science Foundation of Zhejiang Province (No.s LY16B050007 and LQ19B050002), the Program for Science and Technology of Zhejiang Province (No. 2018C37076) and the Science and Technology Plan Project of Jiaxing City, China (No. 2017AY33034 and 2018AY11002) for funding this research.

Compliance with ethical standards

Human sera sample used in this work was obtained from a healthy volunteer. This study was performed on the consent of the healthy volunteer and approved by the Medical Ethics Committee of Xin’an International Hospital (Jiaxing, China). The authors declare that they have no competing interests.

Supplementary material

604_2019_3385_MOESM1_ESM.docx (159 kb)
ESM 1 (DOCX 159 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Biological, Chemical Sciences and EngineeringJiaxing UniversityJiaxingChina
  2. 2.School of Petrochemical EngineeringChangzhou UniversityChangzhouChina

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