Advertisement

Microchimica Acta

, 186:238 | Cite as

Electrostatic assembly of gold nanoparticles on black phosphorus nanosheets for electrochemical aptasensing of patulin

  • Jinqiong Xu
  • Xiujuan Qiao
  • Yuan Wang
  • Qinglin ShengEmail author
  • Tianli YueEmail author
  • Jianbin Zheng
  • Ming Zhou
Original Paper
  • 24 Downloads

Abstract

An aptamer based impedimetric assay for the mycotoxin patulin (PAT) is described. A glassy carbon electrode (GCE) was modified with black phosphorus nanosheets (BP NSs) and modified with PAT aptamer by electrostatic attraction. Detection is based on the variations of electron transfer resistance at the modified electrode surface. This assay can detect PAT over a linear range that extends from 1.0 nM to 1.0 μM with a 0.3 nM detection limit. To improve the performance of the sensor, the BP NS-GCE was further modified with gold nanoparticles and then with thiolated PAT aptamer. This modified electrode, operated at an applied potential of 0.18 V (vs. Ag/AgCl), has a wider linear range (0.1 nM to 10.0 μM) and a lower detection limits (0.03 nM). Both assays were successfully applied to the analysis of (spiked) genuine food samples.

Graphical abstract

Black phosphorus nanosheets (BP NSs) were used to fabricate an aptamer based assay for patulin. To further improve the performance of the electrode, gold nanoparticles (AuNP) were placed on the surface of black phosphorus nanosheets (AuNP-BP NSs) by electrostatic attraction for patulin aptasensing.

Keywords

Electrochemical sensor Impedimetric Electron transfer Two-dimensional nanomaterials Aptamer 

Notes

Acknowledgments

The authors gratefully acknowledge the financial support of this project by the National Science Foundation of China (21575113), the State Key Laboratory of Analytical Chemistry for Life Science (SKLACLS1811), the Natural Science Foundation of Shaanxi Province in China (2017JM2036).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3339_MOESM1_ESM.doc (6.2 mb)
ESM 1 (DOC 6364 kb)

References

  1. 1.
    Marín S, Mateo EM, Sanchis V, Valle-Algarra FM, Ramos AJ, Jiménez M (2011) Patulin contamination in fruit derivatives, including baby food, from the Spanish market. Food Chem 124:563–568CrossRefGoogle Scholar
  2. 2.
    Wu S, Duan N, Zhang W, Zhao S, Wang Z (2016) Screening and development of DNA aptamers as capture probes for colorimetric detection of patulin. Anal Biochem 508:58–64CrossRefGoogle Scholar
  3. 3.
    Zhang G, Liu Z, Fan L, Guo Y (2018) Electrochemical prostate specific antigen aptasensor based on hemin functionalized graphene-conjugated palladium nanocomposites. Microchim Acta 185:159CrossRefGoogle Scholar
  4. 4.
    Li X, Li H, Li X, Zhang Q (2017) Determination of trace patulin in apple-based food matrices. Food Chem 233:290–301CrossRefGoogle Scholar
  5. 5.
    Guo W, Pi F, Zhang H, Sun J, Zhang Y, Sun X (2017) A novel molecularly imprinted electrochemical sensor modified with carbon dots, chitosan, gold nanoparticles for the determination of patulin. Biosens Bioelectron 98:299–304CrossRefGoogle Scholar
  6. 6.
    Welke JE, Hoeltz M, Dottori HA, Noll IB (2009) Quantitative analysis of patulin in apple juice by thin-layer chromatography using a charge coupled device detector. Food Addit Contam A 26:754–758CrossRefGoogle Scholar
  7. 7.
    Beltrán E, Ibáñez M, Sancho JV, Hernandez F (2014) Determination of patulin in apple and derived products by UHPLC-MS/MS. study of matrix effects with atmospheric pressure ionisation sources. Food Chem 142:400–407CrossRefGoogle Scholar
  8. 8.
    Kharandi N, Babri M, Azad J (2013) A novel method for determination of patulin in apple juices by GC-MS. Food Chem 141:1619–1623CrossRefGoogle Scholar
  9. 9.
    Funari R, Della Ventura B, Carrieri R, Morra L, Lahoz E, Gesuele F, Altuccia C, Velotta R (2015) Detection of parathion and patulin by quartz-crystal microbalance functionalized by the photonics immobilization technique. Biosens Bioelectron 67:224–229CrossRefGoogle Scholar
  10. 10.
    Chen YX, Wu X, Huang KJ (2018) A sandwich-type electrochemical biosensing platform for microRNA-21 detection using carbon sphere-MoS2 and catalyzed hairpin assembly for signal amplification. Sensors Actuators B Chem 270:179–186CrossRefGoogle Scholar
  11. 11.
    Chanique GD, Arévalo AH, Zon MA, Fernández H (2013) Eletrochemical reduction of patulin and 5-hydroxymethylfurfural in both neutral and acid non-aqueous media. Their electroanalytical determination in apple juices. Talanta 111:85–92CrossRefGoogle Scholar
  12. 12.
    Roushani M, Nezhadali A, Jalilian Z (2018) An electrochemical chlorpyrifos aptasensor based on the use of a glassy carbon electrode modified with an electropolymerized aptamer-imprinted polymer and gold nanorods. Microchim Acta 185:551CrossRefGoogle Scholar
  13. 13.
    Lei YM, Huang WX, Zhao M, Chai YQ, Yuan R, Zhuo Y (2015) Electrochemiluminescence resonance energy transfer system: mechanism and application in ratiometric aptasensor for lead ion. Anal Chem 87:7787–7794CrossRefGoogle Scholar
  14. 14.
    Nguyen VT, Seo HB, Kim BC, Kim SK, Song CS, Gu MB (2016) Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers. Biosens Bioelectron 86:293–300CrossRefGoogle Scholar
  15. 15.
    Lei W, Liu G, Zhang J, Liu M (2017) Black phosphorus nanostructures: recent advances in hybridization, doping and functionalization. Chem Soc Rev 46:3492–3509CrossRefGoogle Scholar
  16. 16.
    Chen W, Ouyang J, Yi X, Xu Y, Niu C, Zhang W, Wang L, Sheng J, Deng L, Liu Y, Guo S (2018) Black phosphorus Nanosheets as a neuroprotective nanomedicine for neurodegenerative disorder therapy. Adv Mater 30:1703458CrossRefGoogle Scholar
  17. 17.
    Millstone JE, Wei W, Jones MR, Yoo H, Mirkin CA (2008) Iodide ions control seed-mediated growth of anisotropic gold nanoparticles. Nano Lett 8:2526–2529CrossRefGoogle Scholar
  18. 18.
    Wu S, Zhang H, Shi Z, Duan N, Fang C, Dai S, Wang Z (2015) Aptamer-based fluorescence biosensor for chloramphenicol determination using upconversion nanoparticles. Food Control 50:597–604CrossRefGoogle Scholar
  19. 19.
    Saleh TA, Al-Shalalfeh MM, Al-Saadi AA (2018) Silver loaded graphene as a substrate for sensing 2-thiouracil using surface-enhanced Raman scattering. Sensors Actuators B Chem 254:1110–1117CrossRefGoogle Scholar
  20. 20.
    Dhara K, Mahapatra DR (2018) Electrochemical nonenzymatic sensing of glucose using advanced nanomaterials. Microchim Acta 185:49CrossRefGoogle Scholar
  21. 21.
    Lei W, Zhang T, Liu P, Rodriguez JA, Liu G, Liu M (2016) Bandgap-and local field-dependent photoactivity of ag/black phosphorus nanohybrids. ACS Catal 6:8009–8020CrossRefGoogle Scholar
  22. 22.
    Eswaraiah V, Zeng Q, Long Y, Liu Z (2016) Black phosphorus nanosheets: synthesis, characterization and applications. Small 12:3480–3502CrossRefGoogle Scholar
  23. 23.
    Li L, Yu Y, Ye GJ, Ge Q, Ou X, Wu H, Feng D, Chen XH, Zhang Y (2014) Black phosphorus field-effect transistors. Nat Nanotechnol 9:372–377CrossRefGoogle Scholar
  24. 24.
    Fei R, Yang L (2014) Strain-engineering the anisotropic electrical conductance of few-layer black phosphorus. Nano Lett 14:2884–2889CrossRefGoogle Scholar
  25. 25.
    Xu GL, Chen ZH, Zhong GM, Liu YZ, Yang YT, Ma Y, Ren Y, Zuo XB, Wu XH, Zhang XY, Amine K (2016) Nanostructured black phosphorus/Ketjenblack-multiwalled carbon nanotubes composite as high performance anode material for sodium-ion batteries. Nano Lett 16:3955–3965CrossRefGoogle Scholar
  26. 26.
    Lee HU, Lee SC, Won J, Son BC, Choi S, Kim Y, Park SY, Kim HS, Lee YC, Lee J (2015) Stable semiconductor black phosphorus (BP)@ titanium dioxide (TiO2) hybrid photocatalysts. Sci Rep 5:8691CrossRefGoogle Scholar
  27. 27.
    Yu Y, Fan Z (2017) Determination of patulin in apple juice using magnetic solid-phase extraction coupled with high-performance liquid chromatography. Food Addit Contam A 34:273–281Google Scholar
  28. 28.
    Malysheva SV, Di Mavungu JD, Boonen J, De Spiegeleer B, Goryacheva IY, Vanhaecke L, De Saeger S (2012) Improved positive electrospray ionization of patulin by adduct formation: usefulness in liquid chromatography-tandem mass spectrometry multi-mycotoxin analysis. J Chromatogr A 1270:334–339CrossRefGoogle Scholar
  29. 29.
    Pennacchio A, Ruggiero G, Staiano M, Piccialli G, Oliviero G, Lewkowicz A, Synak A, Bojarski P, Auria SD (2014) A surface plasmon resonance based biochip for the detection of patulin toxin. Opt Mater 36:1670–1675CrossRefGoogle Scholar
  30. 30.
    Bagheri N, Khataee A, Habibi B, Hassanzadeh J (2018) Mimetic ag nanoparticle/Zn-based MOF nanocomposite (AgNPs@ ZnMOF) capped with molecularly imprinted polymer for the selective detection of patulin. Talanta 179:710–718CrossRefGoogle Scholar
  31. 31.
    Wu Z, Xu E, Jin Z, Irudayaraj J (2018) An ultrasensitive aptasensor based on fluorescent resonant energy transfer and exonuclease-assisted target recycling for patulin detection. Food Chem 249:136–142CrossRefGoogle Scholar
  32. 32.
    Zhang W, Han Y, Chen X, Luo X, Wang J, Yue T, Li Z (2017) Surface molecularly imprinted polymer capped Mn-doped ZnS quantum dots as a phosphorescent nanosensor for detecting patulin in apple juice. Food Chem 232:145–154CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Chemistry & Materials Science, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education/Shaanxi Provincial Key Laboratory of Electroanalytical ChemistryNorthwest UniversityXi’anChina
  2. 2.College of Food Science and EngineeringNorthwest UniversityXi’anChina
  3. 3.State Key Laboratory of Analytical Chemistry for Life ScienceNanjing UniversityNanjingChina
  4. 4.Key Laboratory of Polyoxometalate Science of Ministry of Education, Faculty of Chemistry, and National & Local United Engineering Laboratory for Power BatteriesNortheast Normal UniversityChangchunPeople’s Republic of China

Personalised recommendations