Microchimica Acta

, 186:238 | Cite as

Electrostatic assembly of gold nanoparticles on black phosphorus nanosheets for electrochemical aptasensing of patulin

  • Jinqiong Xu
  • Xiujuan Qiao
  • Yuan Wang
  • Qinglin ShengEmail author
  • Tianli YueEmail author
  • Jianbin Zheng
  • Ming Zhou
Original Paper


An aptamer based impedimetric assay for the mycotoxin patulin (PAT) is described. A glassy carbon electrode (GCE) was modified with black phosphorus nanosheets (BP NSs) and modified with PAT aptamer by electrostatic attraction. Detection is based on the variations of electron transfer resistance at the modified electrode surface. This assay can detect PAT over a linear range that extends from 1.0 nM to 1.0 μM with a 0.3 nM detection limit. To improve the performance of the sensor, the BP NS-GCE was further modified with gold nanoparticles and then with thiolated PAT aptamer. This modified electrode, operated at an applied potential of 0.18 V (vs. Ag/AgCl), has a wider linear range (0.1 nM to 10.0 μM) and a lower detection limits (0.03 nM). Both assays were successfully applied to the analysis of (spiked) genuine food samples.

Graphical abstract

Black phosphorus nanosheets (BP NSs) were used to fabricate an aptamer based assay for patulin. To further improve the performance of the electrode, gold nanoparticles (AuNP) were placed on the surface of black phosphorus nanosheets (AuNP-BP NSs) by electrostatic attraction for patulin aptasensing.


Electrochemical sensor Impedimetric Electron transfer Two-dimensional nanomaterials Aptamer 



The authors gratefully acknowledge the financial support of this project by the National Science Foundation of China (21575113), the State Key Laboratory of Analytical Chemistry for Life Science (SKLACLS1811), the Natural Science Foundation of Shaanxi Province in China (2017JM2036).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3339_MOESM1_ESM.doc (6.2 mb)
ESM 1 (DOC 6364 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Chemistry & Materials Science, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education/Shaanxi Provincial Key Laboratory of Electroanalytical ChemistryNorthwest UniversityXi’anChina
  2. 2.College of Food Science and EngineeringNorthwest UniversityXi’anChina
  3. 3.State Key Laboratory of Analytical Chemistry for Life ScienceNanjing UniversityNanjingChina
  4. 4.Key Laboratory of Polyoxometalate Science of Ministry of Education, Faculty of Chemistry, and National & Local United Engineering Laboratory for Power BatteriesNortheast Normal UniversityChangchunPeople’s Republic of China

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