Electrostatic assembly of gold nanoparticles on black phosphorus nanosheets for electrochemical aptasensing of patulin
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An aptamer based impedimetric assay for the mycotoxin patulin (PAT) is described. A glassy carbon electrode (GCE) was modified with black phosphorus nanosheets (BP NSs) and modified with PAT aptamer by electrostatic attraction. Detection is based on the variations of electron transfer resistance at the modified electrode surface. This assay can detect PAT over a linear range that extends from 1.0 nM to 1.0 μM with a 0.3 nM detection limit. To improve the performance of the sensor, the BP NS-GCE was further modified with gold nanoparticles and then with thiolated PAT aptamer. This modified electrode, operated at an applied potential of 0.18 V (vs. Ag/AgCl), has a wider linear range (0.1 nM to 10.0 μM) and a lower detection limits (0.03 nM). Both assays were successfully applied to the analysis of (spiked) genuine food samples.
KeywordsElectrochemical sensor Impedimetric Electron transfer Two-dimensional nanomaterials Aptamer
The authors gratefully acknowledge the financial support of this project by the National Science Foundation of China (21575113), the State Key Laboratory of Analytical Chemistry for Life Science (SKLACLS1811), the Natural Science Foundation of Shaanxi Province in China (2017JM2036).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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