Colorimetric enzymatic determination of glucose based on etching of gold nanorods by iodine and using carbon quantum dots as peroxidase mimics
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Carbon quantum dots (CQDs) with peroxidase-mimicking activity were successfully prepared from litchi rind. A colorimetric method for glucose determination was developed based on etching of gold nanorods (GNRs) using CQDs as peroxidase mimetic. The glucose oxidase-catalyzed oxidation of glucose leads to the generation of H2O2 which oxidizes added iodide under formation of elemental iodine under the catalytic action of CQDs. Iodine then etches the GNRs along the longitudinal direction due to the higher reaction activities at the tips of GNRs. This results in a stepwise decrease in the maximum absorption wavelength of the GNRs, from initially 953 nm to finally 645 nm. Under the optimized conditions, the shift in the maximum absorption wavelength decreases linearly in the 0.01–2.0 mM glucose concentration range, and the detection limit is 3.0 μM. Importantly, this method was applied to the determination of glucose in human serum. It is perceived that the CQDs are valuable peroxidase mimics due to their ease of preparation, low costs and stable catalytic activity.
KeywordsSpectrophotometry Optical assay Nanomaterial Peroxidase mimetic Glucose oxidase Enzyme mimicking Blood plasma Glucose detection
The present work was supported by the Natural Science Foundation of China (21567002) and the Opening Project of Guangxi Key Laboratory of Bio Refinery (GXKLB-201801).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 5.Ding L, Gong Z, Yan M, Yu J, Song X (2017) Determination of glucose by using fluorescent silicon nanoparticles and an inner filter caused by peroxidase-induced oxidation of o-phenylenediamine by hydrogen peroxide. Microchim Acta 184:4531–4536. https://doi.org/10.1007/s00604-017-2445-3 CrossRefGoogle Scholar
- 10.Hallaj T, Amjadi M, Song Z, Bagheri R (2018) Strong enhancement of the chemiluminescence of the Cu(II)-H2O2 system on addition of carbon nitride quantum dots, and its application to the detection of H2O2 and glucose. Microchim Acta 185:67. https://doi.org/10.1007/s00604-017-2547-y CrossRefGoogle Scholar
- 12.Luo F, Lin Y, Zheng L, Lin X, Chi Y (2015) Encapsulation of hemin in metal organic frameworks for catalyzing the Chemiluminescence reaction of the H2O2-Luminol system and detecting glucose in the neutral condition. ACS Appl Mater Interfaces 7:11322–11329. https://doi.org/10.1021/acsami.5b01706 CrossRefPubMedGoogle Scholar
- 18.Wang Y, Zhang D, Wang J (2018) Metastable alpha-AgVO3 microrods as peroxidase mimetics for colorimetric determination of H2O2. Microchim Acta 185(1). https://doi.org/10.1007/s00604-017-2562-z
- 23.Rastogi L, Karunasagar D, Sashidhar RB, Giri A (2017) Peroxidase-like activity of gum kondagogu reduced/stabilized palladium nanoparticles and its analytical application for colorimetric detection of glucose in biological samples. Sensors Actuators B Chem 240:1182–1188. https://doi.org/10.1016/j.snb.2016.09.066 CrossRefGoogle Scholar
- 33.Lin L, Song X, Chen Y, Rong M, Zhao T, Wang Y, Jiang Y, Chen X (2015) Intrinsic peroxidase-like catalytic activity of nitrogen-doped graphene quantum dots and their application in the colorimetric detection of H2O2 and glucose. Anal Chim Acta 869:89–95. https://doi.org/10.1016/j.aca.2015.02.024 CrossRefPubMedGoogle Scholar
- 41.Sahoo NK, Das S, Jana GC, Aktara MN, Patra A, Maji A, Beg M, Jha PK, Hossain M (2019) Eco-friendly synthesis of a highly fluorescent carbon dots from spider silk and its application towards Hg (II) ions detection in real sample and living cells. Microchem J 144:479–488. https://doi.org/10.1016/j.microc.2018.10.006 CrossRefGoogle Scholar
- 47.Shen S, Jia M, Tang Z, Chang S, Shi P, Yang J (2018) Preparation and application of Mn-doped Zn0.5Cd0.5S @ ZnS nanorods with high quantum yield as sensitive fluorescence probe for detection of glucose. Mater Res Bull 106:471–477. https://doi.org/10.1016/j.materresbull.2018.06.043 CrossRefGoogle Scholar
- 49.Shin HY, Kim B-G, Cho S, Lee J, Na HB, Kim MI (2017) Visual determination of hydrogen peroxide and glucose by exploiting the peroxidase-like activity of magnetic nanoparticles functionalized with a poly(ethylene glycol) derivative. Microchim Acta 184:2115–2122. https://doi.org/10.1007/s00604-017-2198-z CrossRefGoogle Scholar
- 50.Chen J, Chen Q, Chen J, Qiu H (2016) Magnetic carbon nitride nanocomposites as enhanced peroxidase mimetics for use in colorimetric bioassays, and their application to the determination of H2O2 and glucose. Microchim Acta 183:3191–3199. https://doi.org/10.1007/s00604-016-1972-7 CrossRefGoogle Scholar
- 51.Tran HV, Nguyen TV, Nguyen ND, Piro B, Huynh CD (2018) A nanocomposite prepared from FeOOH and N-doped carbon nanosheets as a peroxidase mimic, and its application to enzymatic sensing of glucose in human urine. Microchim Acta 185:270. https://doi.org/10.1007/s00604-018-2804-8 CrossRefGoogle Scholar