Microchimica Acta

, 186:168 | Cite as

A copper(II)/cobalt(II) organic gel with enhanced peroxidase-like activity for fluorometric determination of hydrogen peroxide and glucose

  • Ting Ting Zhao
  • Zhong Wei Jiang
  • Shu Jun Zhen
  • Cheng Zhi HuangEmail author
  • Yuan Fang LiEmail author
Original Paper


A bimetallic organic gel was prepared by mixing the bridging ligand 2,4,6-tri(4-carboxyphenyl)-1,3,5-triazine with Cu(II) and Co(II) ions at room temperature. The resulting metal-organic gel (MOG) shows enhanced peroxidase-like activity, most likely due to the synergetic redox cycling between Co(III)/Co(II) and Cu(II)/Cu(I) pairs. These accelerate interfacial electron transfer and generation of hydroxy radicals. The MOG can catalyze the reaction of H2O2 with terephthalic acid (TPA), producing a blue fluorescence product with the maximum excitation/emission at 315/446 nm. The enzyme mimic was used to design a fluorometric method for H2O2 that has a 81 nM detection limit. H2O2 is also formed by glucose oxidase-assisted oxidation of glucose by oxygen, and an assay for glucose was worked out based on the above method. It has a 0.33 μM detection limit. This study may open up a new avenue to design and synthesize nanomaterial-based biomimetic catalysts with multiple metal synergistically enhanced catalytic activity for potential applications in biocatalysis, bioassays and nano-biomedicine.

Graphical abstracts

Schematic presentation of the synergic catalytic effect of Cu(II)/Co(II) bimetallic organic gel promoted by the redox cycle between Co(III)/Co(II) and Cu(II)/Cu(I) pairs. The bimetallic organic gel can catalyze the reaction of H2O2 with terephthalic acid, thereby producing a blue-fluorescent product.


Peroxidase mimetic Redox cycle Synergistic effect Metal-organic gel Enzyme mimic Terephthalic acid 2-hydroxyterephthalic acid Fluorescence Catalysis Hydroxy radicals 



The authors are grateful to the National Natural Science Foundation of China (NSFC, No. 21575117).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3290_MOESM1_ESM.docx (1.3 mb)
ESM 1 (DOCX 1338 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical EngineeringSouthwest UniversityChongqingPeople’s Republic of China
  2. 2.College of Pharmaceutical ScienceSouthwest UniversityChongqingPeople’s Republic of China

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