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Microchimica Acta

, 186:241 | Cite as

Fluorometric determination of the activity of the biomarker terminal deoxynucleotidyl transferase via the enhancement of the fluorescence of silver nanoclusters by in-situ grown DNA tails

  • Bao-Zhu Chi
  • Chen-Lu Wang
  • Zhi-Qiao Wang
  • Ting Pi
  • Xiao-Li Zhong
  • Chao-Qun Deng
  • Yu-Chuan Feng
  • Zhi-Mei LiEmail author
Original Paper
  • 83 Downloads

Abstract

The activity of terminal deoxynucleotidyl transferase (TdTase) is a biomarker for routine diagnosis of acute leukemia. A method has been developed for the determination of TdTase activity. It is based on the use of silver nanoclusters (AgNCs) whose yellow fluorescence is enhanced by an in-situ grown DNA tail of TdTase-polymerized and guanine-rich DNA at the 3′ end of a hairpin DNA. The fluorescence, best measured at excitation/emission peaks of 530/585 nm, increases linearly in the 1 to 35 mU mL−1 TdTase activity range. The detection limit is 0.8 mU mL−1. The method is cost-efficient, selective and convenient. It integrates enhancement of the fluorescence of AgNCs and target recognition into a single process.

Graphical abstract

Schematic presentation of a method for determination of TdTase activity. It is based on AgNCs fluorescence enhanced by in-situ grown TdTase-polymerized G-rich DNA tail. The method integrates AgNCs fluorescence enhancement and the target recognition into a single process.

Keywords

TdTase Enhancement in situ AgNCs Luminescent probes Biomarkers Turn on Fluorescence Acute leukemia Hairpin DNA Guanine-rich 

Notes

Acknowledgements

This research was supported by the National Natural Science Foundation of China (21365015), and the Jiangxi Province Natural Science Foundation (20171BAB213014).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3288_MOESM1_ESM.doc (146 kb)
ESM 1 (DOC 145 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Bao-Zhu Chi
    • 1
  • Chen-Lu Wang
    • 1
  • Zhi-Qiao Wang
    • 1
  • Ting Pi
    • 1
  • Xiao-Li Zhong
    • 1
  • Chao-Qun Deng
    • 1
  • Yu-Chuan Feng
    • 1
  • Zhi-Mei Li
    • 1
    Email author
  1. 1.Department of ChemistryNanchang UniversityNanchangPeople’s Republic of China

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