Fluorometric determination of the activity of the biomarker terminal deoxynucleotidyl transferase via the enhancement of the fluorescence of silver nanoclusters by in-situ grown DNA tails
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The activity of terminal deoxynucleotidyl transferase (TdTase) is a biomarker for routine diagnosis of acute leukemia. A method has been developed for the determination of TdTase activity. It is based on the use of silver nanoclusters (AgNCs) whose yellow fluorescence is enhanced by an in-situ grown DNA tail of TdTase-polymerized and guanine-rich DNA at the 3′ end of a hairpin DNA. The fluorescence, best measured at excitation/emission peaks of 530/585 nm, increases linearly in the 1 to 35 mU mL−1 TdTase activity range. The detection limit is 0.8 mU mL−1. The method is cost-efficient, selective and convenient. It integrates enhancement of the fluorescence of AgNCs and target recognition into a single process.
KeywordsTdTase Enhancement in situ AgNCs Luminescent probes Biomarkers Turn on Fluorescence Acute leukemia Hairpin DNA Guanine-rich
This research was supported by the National Natural Science Foundation of China (21365015), and the Jiangxi Province Natural Science Foundation (20171BAB213014).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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