Advertisement

Microchimica Acta

, 186:156 | Cite as

A hybrid material composed of guanine-rich single stranded DNA and cobalt(III) oxyhydroxide (CoOOH) nanosheets as a fluorescent probe for ascorbic acid via formation of a complex between G-quadruplex and thioflavin T

  • Shi Gang Liu
  • Dan Luo
  • Lei Han
  • Nian Bing LiEmail author
  • Hong Qun LuoEmail author
Original Paper
  • 24 Downloads

Abstract

A hybrid material composed of guanine-rich single stranded DNA (G-rich ssDNA) and cobalt oxyhydroxide (CoOOH) nanosheets is used as a nanoprobe for fluorometric turn-on detection of ascorbic acid (AA). The CoOOH nanosheets function as a recognition component for AA. The G-rich ssDNA is used to produce a G-quadruplex, and the G-quadruplex/thioflavin T (ThT) complex acts as a fluorescent reporter. In the absence of AA, p-phenylenediamine (PPD) is oxidized to form oxPPD which has a dark red color. It causes the fluorescence of the G-quadruplex/ThT complex to be quenched. However, in the presence of AA, the CoOOH nanosheets of the nanoprobe are preferentially reduced by AA. Hence, PPD is not oxidized, and fluorescence is not quenched. A fluorometric turn-on method was developed based on these findings. It has a detection limit of 94 nM and works in the concentration range from 1 to 10 and 20 to 80 μM. This method was applied to the determination of AA in (spiked) fruit juice samples.

Graphical abstract

Schematic presentation of a fluorescent assay of ascorbic acid (AA) is established using a nanoprobe composed of guanine-rich single stranded DNA (G-rich ssDNA) and cobalt oxyhydroxide (CoOOH) nanosheets. It is based on competitive reduction of CoOOH by p-phenylenediamine (PPD) and AA. Thioflavine T (ThT) induces the formation of fluorescent G-quadruplex/ThT complex. The oxidized form of PPD (oxPPD) can quench the fluorescence via fluorescence resonance energy transfer (FRET), but AA suppresses quenching.

Keywords

Ascorbic acid G-quadruplex Cobalt oxyhydroxide Thioflavin T Fluorometric detection p-Phenylenediamine 

Notes

Acknowledgments

Authors acknowledge financial support for this work from the National Natural Science Foundation of China (No. 21675131), the Natural Science Foundation of Chongqing (No. CSTC-2015jcyjB50001), and the Fundamental Research Funds for the Central Universities (XDJK2018D012).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2019_3279_MOESM1_ESM.doc (3.3 mb)
ESM 1 (DOC 3.33 mb)

References

  1. 1.
    Patil BS, Jayaprakasha GK, Chidambara Murthy KN, Vikram A (2009) Bioactive compounds: historical perspectives, opportunities, and challenges. J Agric Food Chem 57:8142–8160CrossRefGoogle Scholar
  2. 2.
    Zhu X, Zhao T, Nie Z, Liu Y, Yao S (2015) Non-redox modulated fluorescence strategy for sensitive and selective ascorbic acid detection with highly photoluminescent nitrogen-doped carbon nanoparticles via solid-state synthesis. Anal Chem 87:8524–8530CrossRefGoogle Scholar
  3. 3.
    Liu J, Chen Y, Wang W, Feng J, Liang M, Ma S, Chen X (2016) "Switch-on" fluorescent sensing of ascorbic acid in food samples based on carbon quantum dots-MnO2 probe. J Agric Food Chem 64:371–380CrossRefGoogle Scholar
  4. 4.
    Arya SP, Mahajan M, Jain P (2000) Non-spectrophotometric methods for the determination of vitamin C. Anal Chim Acta 417:1–14CrossRefGoogle Scholar
  5. 5.
    Cui W, Wang Y, Yang D, Du J (2017) Fluorometric determination of ascorbic acid by exploiting its deactivating effect on the oxidase–mimetic properties of cobalt oxyhydroxide nanosheets. Microchim Acta 184:4749–4755CrossRefGoogle Scholar
  6. 6.
    Wang G, Chen Z, Chen L (2011) Mesoporous silica-coated gold nanorods: towards sensitive colorimetric sensing of ascorbic acid via target-induced silver overcoating. Nanoscale 3:1756–1759CrossRefGoogle Scholar
  7. 7.
    Deng K, Li X, Huang H (2016) A glassy carbon electrode modified with a nickel(II) norcorrole complex and carbon nanotubes for simultaneous or individual determination of ascorbic acid, dopamine, and uric acid. Microchim Acta 183:2139–2145CrossRefGoogle Scholar
  8. 8.
    Wang Z, Teng X, Lu C (2012) Carbonate interlayered hydrotalcites-enhanced peroxynitrous acid chemiluminescence for high selectivity sensing of ascorbic acid. Analyst 137:1876–1881CrossRefGoogle Scholar
  9. 9.
    Frenich AG, Torres ME, Vega AB, Vidal JL, Bolanos PP (2005) Determination of ascorbic acid and carotenoids in food commodities by liquid chromatography with mass spectrometry detection. J Agric Food Chem 53:7371–7376CrossRefGoogle Scholar
  10. 10.
    Zhao M, Yu H, He Y (2018) A dynamic multichannel colorimetric sensor array for highly effective discrimination of ten explosives. Sensors Actuators B Chem 283:329–333CrossRefGoogle Scholar
  11. 11.
    Huang W, Zhou Y, Du J, Deng Y, He Y (2018) Versatile visual logic operations based on plasmonic switching in label-free molybdenum oxide nanomaterials. Anal Chem 90:2384–2388CrossRefGoogle Scholar
  12. 12.
    Zhou Y, Huang W, He Y (2018) pH-Induced silver nanoprism etching-based multichannel colorimetric sensor array for ultrasensitive discrimination of thiols. Sensors Actuators B Chem 270:187–191CrossRefGoogle Scholar
  13. 13.
    Ganiga M, Cyriac J (2016) An ascorbic acid sensor based on cadmium sulphide quantum dots. Anal Bioanal Chem 408:3699–3706CrossRefGoogle Scholar
  14. 14.
    Li L, Wang C, Liu K, Wang Y, Liu K, Lin Y (2015) Hexagonal cobalt oxyhydroxide-carbon dots hybridized surface: high sensitive fluorescence turn-on probe for monitoring of ascorbic acid in rat brain following brain ischemia. Anal Chem 87:3404–3411CrossRefGoogle Scholar
  15. 15.
    Meng H, Yang D, Tu Y, Yan J (2017) Turn-on fluorescence detection of ascorbic acid with gold nanolcusters. Talanta 165:346–350CrossRefGoogle Scholar
  16. 16.
    Yue D, Zhao D, Zhang J, Zhang L, Jiang K, Zhang X, Cui Y, Yang Y, Chen B, Qian G (2017) A luminescent cerium metal-organic framework for the turn-on sensing of ascorbic acid. Chem Commun 53:11221–11224CrossRefGoogle Scholar
  17. 17.
    Rong M, Lin L, Song X, Wang Y, Zhong Y, Yan J, Feng Y, Zeng X, Chen X (2015) Fluorescence sensing of chromium (VI) and ascorbic acid using graphitic carbon nitride nanosheets as a fluorescent “switch”. Biosens Bioelectron 68:210–217CrossRefGoogle Scholar
  18. 18.
    Liu SG, Li N, Han L, Li LJ, Li NB, Luo HQ (2018) Size-dependent modulation of fluorescence and light scattering: a new strategy for development of ratiometric sensing. Mater Horiz 5:454–460CrossRefGoogle Scholar
  19. 19.
    Sen D, Gilbert W (1988) Formation of parallel four-stranded complexes by guanine-rich motifs in DNA and its implications for meiosis. Nature 334:364–366CrossRefGoogle Scholar
  20. 20.
    Zhao H, Dong J, Zhou F, Li B (2015) G-quadruplex − based homogenous fluorescence platform for ultrasensitive DNA detection through isothermal cycling and cascade signal amplification. Microchim Acta 182:2495–2502CrossRefGoogle Scholar
  21. 21.
    Mohanty J, Barooah N, Dhamodharan V, Harikrishna S, Pradeepkumar PI, Bhasikuttan AC (2013) Thioflavin T as an efficient inducer and selective fluorescent sensor for the human telomeric G-quadruplex DNA. J Am Chem Soc 135:367–376CrossRefGoogle Scholar
  22. 22.
    Du YC, Zhu LN, Kong DM (2016) Label-free thioflavin T/G-quadruplex-based real-time strand displacement amplification for biosensing applications. Biosens Bioelectron 86:811–817CrossRefGoogle Scholar
  23. 23.
    Khusbu FY, Zhou X, Chen H, Ma C, Wang K (2018) Thioflavin T as a fluorescence probe for biosensing applications. Trends Anal Chem 109:1–18CrossRefGoogle Scholar
  24. 24.
    Yang Y, Cen Y, Deng WJ, Yu RQ, Chen TT, Chu X (2016) An aptasensor based on cobalt oxyhydroxide nanosheets for the detection of thrombin. Anal Methods 8:7199–7203CrossRefGoogle Scholar
  25. 25.
    Li N, Li Y, Han Y, Pan W, Zhang T, Tang B (2014) A highly selective and instantaneous nanoprobe for detection and imaging of ascorbic acid in living cells and in vivo. Anal Chem 86:3924–3930CrossRefGoogle Scholar
  26. 26.
    Liu SG, Han L, Li N, Xiao N, Ju YJ, Li NB, Luo HQ (2018) A fluorescence and colorimetric dual-mode assay of alkaline phosphatase activity via destroying oxidase-like CoOOH nanoflakes. J Mater Chem B 6:2843–2850CrossRefGoogle Scholar
  27. 27.
    Huang J, Shang Q, Huang Y, Tang F, Zhang Q, Liu Q, Jiang S, Hu F, Liu W, Luo Y, Yao T, Jiang Y, Pan Z, Sun Z, Wei S (2016) Oxyhydroxide nanosheets with highly efficient electron-hole pair separation for hydrogen evolution. Angew Chem Int Ed 55:2137–2141CrossRefGoogle Scholar
  28. 28.
    Zhu L, Wu W, Zhu Y, Tang W, Wu Y (2015) Composite of CoOOH nanoplates with multiwalled carbon nanotubes as superior cathode material for supercapacitors. J Phys Chem C 119:7069–7075CrossRefGoogle Scholar
  29. 29.
    Jiao K, Sun W, Zhang S, Sun G (2000) Application of p-phenylenediamine as an electrochemical substrate in peroxidase-mediated voltammetric enzyme immunoassay. Anal Chim Acta 413:71–78CrossRefGoogle Scholar
  30. 30.
    Sun J, Zhao J, Wang L, Li H, Yang F, Yang X (2018) Inner filter effect-based sensor for horseradish peroxidase and its application to fluorescence immunoassay. ACS Sens 3:183–190CrossRefGoogle Scholar
  31. 31.
    Chang Y, Zhang Z, Liu H, Wang N, Tang J (2016) Cobalt oxyhydroxide nanoflake based fluorescence sensing platform for label-free detection of DNA. Analyst 141:4719–4724CrossRefGoogle Scholar
  32. 32.
    Li BL, Wang J, Zou HL, Garaj S, Lim CT, Xie J, Li NB, Leong DT (2016) Low-dimensional transition metal Dichalcogenide nanostructures based sensors. Adv Funct Mater 26:7034–7056CrossRefGoogle Scholar
  33. 33.
    Zu F, Yan F, Bai Z, Xu J, Wang Y, Huang Y, Zhou X (2017) The quenching of the fluorescence of carbon dots: a review on mechanisms and applications. Microchim Acta 184:1899–1914CrossRefGoogle Scholar
  34. 34.
    Liu SG, Luo D, Li N, Zhang W, Lei JL, Li NB, Luo HQ (2016) Water-soluble nonconjugated polymer nanoparticles with strong fluorescence emission for selective and sensitive detection of nitro-explosive picric acid in aqueous medium. ACS Appl Mater Interfaces 8:21700–21709CrossRefGoogle Scholar
  35. 35.
    Raut S, Rich R, Fudala R, Butler S, Kokate R, Gryczynski Z, Luchowski R, Gryczynski I (2014) Resonance energy transfer between fluorescent BSA protected au nanoclusters and organic fluorophores. Nanoscale 6:385–391CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical EngineeringSouthwest UniversityChongqingPeople’s Republic of China

Personalised recommendations