Magnetite nanoparticles coated with mercaptosuccinic acid-modified mesoporous titania as a hydrophilic sorbent for glycopeptides and phosphopeptides prior to their quantitation by LC-MS/MS
A hydrophilic material consisting of a magnetite core coated with mercaptosuccinic acid modified mesoporous titania (denoted as Fe3O4@mTiO2-MSA) has been fabricated. It is shown to be a viable sorbent for capturing glycopeptides and phosphopeptides. The sorbent combines the features of metal oxide-based affinity chromatography and of hydrophilic interaction liquid chromatography (HILIC) with the advantages of using mesoporous titania. The use of magnetic microspheres provides magnetic response and simplifies separation. Following elution with 10% ammonia, the peptides were submitted to LC-MS/MS analysis. The method enabled 327 phosphopeptides and 65 glycopeptides to be identified in three isolated replicates of merely 5 μL samples of human saliva. Among them, the phosphorylation sites and glycosylation sites were detected in 20 peptide segments.
KeywordsMetal oxide affinity chromatography Hydrophilic interaction liquid chromatography Proteomics Functionalized nanomaterials Simultaneous enrichment Human saliva Mass spectrometry Phosphorylation Glycosylation Post-translational modification
This work was financially supported by National Key R&D Program of China (2018YFA0507501) and the National Natural Science Foundation of China (21425518).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 4.Bajaj SO (2015) A de novo approach towards glycosylation using iterative Pd-/B-dual catalysis: applications in natural products synthesis, digitoxin analogues, oligomannose motifs and their SAR studies. J Appl Phys 76(10):5760–5763Google Scholar
- 28.Wang Y, Hai X, E S CM, Yang T, Wang J (2018) Boronic acid functionalized g-C3N4 nanosheets for ultrasensitive and selective sensing of glycoprotein in the physiological environment. In: NanoscaleGoogle Scholar
- 35.Xu Y, Bailey UM, Punyadeera C, Schulz BL (2014) Identification of salivary N-glycoproteins and measurement of glycosylation site occupancy by boronate glycoprotein enrichment and liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom 28(5):471–482CrossRefGoogle Scholar