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Microchimica Acta

, 186:122 | Cite as

A fluorescent probe composed of quantum dot labeled aptamer and graphene oxide for the determination of the lipopolysaccharide endotoxin

  • Lu-xin Wen
  • Jun-jiang LvEmail author
  • Li Chen
  • Shun-bo Li
  • Xiao-jing Mou
  • Yi XuEmail author
Original Paper

Abstract

Endotoxins are complex lipopolysaccharides (LPS) and key components of the outer cell membrane of Gram-negative bacteria. The authors report on a fluorescent aptamer-based probe for the determination of LPS of Gram-negative bacteria. An aptamer against LPS was fluorescently labeled with CdSe/ZnS quantum dots. Its emission is quenched on addition of graphene oxide (GO). On addition of LPS, the aptamer binds LPS and GO is released. This results in the recovery of fluorescence, typically measured at excitation/emission wavelengths of 495/543 nm. The probe responds to LPS in the 10–500 ng·mL−1 concentration range, and the detection limit is 8.7 ng·mL−1. It can be used for selective detection of LPS from different Gram-negative bacteria, in the presence of biological interferents.

Graphical abstract

Schematic presentation of a green fluorescent probe comprised of an aptamer labelled with CdSe/ZnS quantum dots and of graphene oxide. Lipopolysaccharides bind to the aptamer and release graphene oxide to result in fluorescence recovery, which is measured at an emission wavelength 543 nm.

Keywords

Biosensor Gram-negative bacteria DNA Fluorescence quenching Resonance energy transfer CdSe/ZnS quantum dots Fluorescence turn-on Receptor 

Notes

Acknowledgements

This work was financially supported by National Natural Science Foundation of China (No.21375156), National High Technology Research and Development Program of China (Ministry of Science and Technology 863 Plan)(2015AA021104), Frontier Research Key Projects of Chongqing Science and Technology Committee (cstc2015jcyjBX0010), and Fundamental Research Funds for the Central Universities (No. 10611CDJXZ238826).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3218_MOESM1_ESM.docx (2.4 mb)
ESM 1 (DOCX 2446 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Disciplines Lab of Novel Micro-nano Devices and System TechnologyChongqing UniversityChongqingChina
  2. 2.School of Chemistry and Chemical EngineeringChongqing UniversityChongqingChina
  3. 3.National Center for International Research of Micro/Nano-System and New Material TechnologyChongqing UniversityChongqingChina
  4. 4.Collaborative Innovation Center for Brain ScienceChongqing UniversityChongqingPeople’s Republic of China

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