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Microchimica Acta

, 186:129 | Cite as

A nanocomposite consisting of graphene oxide, zeolite imidazolate framework 8, and a molecularly imprinted polymer for (multiple) fiber solid phase microextraction of sterol and steroid hormones prior to their quantitation by HPLC

  • Roya MirzajaniEmail author
  • Fatemeh Kardani
  • Zahra Ramezani
Original Paper
  • 16 Downloads

Abstract

A method is described for multiple monolithic fiber solid-phase microextraction of five sterol and steroid hormones from complex food samples. A composite was prepared from graphene oxide, a metal-organic framework (ZIF-8) and a molecularly imprinted polymers was deposited on a single thin fiber. Four thin fibers were combined to obtain a fiber bundle. The nanocomposite was characterized by Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy. The parameters affecting the extraction were optimized by Box-Behnken design. Following elution with methanol, the analytes progesterone, testosterone, β-sitosterol, cholesterol and campesterol were quantified via HPLC. Response is linear in the 0.01–1000 μg L−1 concentration range, and limits of detection range from 3 to 5 ng L−1. The method was successfully applied to the determination of the five analytes in spiked samples of white meat, egg yolks and vegetables. The relative mean recoveries ranged from 95.0% to 101.0%.

Graphical abstract

Schematic presentation of a monolith molecularly imprinting polymer (MIP) fiber fabrication for solid phase microextraction (SPME) of sterol and steroid hormones. The fiber was synthesis using graphene oxide and metal-organic framework, ZIF-8, composite by imprinting technique and progesterone as template. Four fibers were combined to obtain a fiber bundle. Then, multiple fiber solid-phase microextraction was employed for determination of analytes by coupling with HPLC/UV detection.

Keywords

Sterol Steroid hormones Graphene oxide Metal–organic frameworks Molecularly imprinted polymer Multiple monolithic fiber Microextraction HPLC 

Notes

Acknowledgements

The Authors greatly appreciate the financial support of this work by Shahid Chamran University of Ahvaz Research Council.

Compliance with ethical standards

The authors declare that they have no competing interests.

Supplementary material

604_2018_3217_MOESM1_ESM.doc (1.1 mb)
ESM 1 (DOC 1.05 mb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Roya Mirzajani
    • 1
    Email author
  • Fatemeh Kardani
    • 1
  • Zahra Ramezani
    • 2
  1. 1.Chemistry Department, College of ScienceShahid Chamran University of AhvazAhvazIran
  2. 2.Faculty of PharmacyAhvaz Jundishapur University of Medical SciencesAhvazIran

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