Gold nanoparticle-loaded hollow Prussian Blue nanoparticles with peroxidase-like activity for colorimetric determination of L-lactic acid
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The intrinsic peroxidase-like activity of hollow Prussian Blue nanoparticle-loaded with gold nanoparticles (Au@HMPB NPs) were applied to oxidize the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2 to give a blue-green coloration. The morphology of the Au@HMPB NPs and its peroxidase-mimicking activity was characterized in detail. The catalytic activity follows Michaelis-Menten kinetics and is higher than that of HMPB NPs not loaded with gold nanoparticles. The NPs were employed to detect L-lactic acid colorimetrically (at 450 nm) via detection of H2O2 that is generated during enzymatic oxidation by L-lactate oxidase (LOx). The limit of detection is 4.2 μM. The assay was successfully applied to the quantitation of L-lactic acid in spiker human serum samples.
KeywordsTetramethylbenzidine Human serum sample Lactate oxidase Gold nanoparticles
The authors thank the support of this work by the National Natural Science Foundation of China (No. 21575165).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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