Microchimica Acta

, 186:90 | Cite as

A glassy carbon electrode modified with graphene oxide, poly(3,4-ethylenedioxythiophene), an antifouling peptide and an aptamer for ultrasensitive detection of adenosine triphosphate

  • Zhenjiang Li
  • Jifang Yin
  • Chenghai Gao
  • Liying Sheng
  • Alan Meng
Original Paper


An antifouling aptasensor is described for voltammetric determination of adenosine triphosphate (ATP). A glassy carbon electrode (GCE) was modified with a graphene oxide and poly(3,4-ethylenedioxythiophene) (GO-PEDOT) composite film by electrodeposition. Next, the zwitterionic peptide (EKEKEKE) was attached. It forms an antifouling layer on the modified GCE and serves as the support for subsequent aptamer immobilization. The resulting aptasensor typically is operated at a potential of 0.18 V (vs. SCE) using hexacyanoferrate as the electrochemical probe. It has a linear response in the 0.1 pM to 1.0 μM ATP concentration range, a 0.03 pM detection limit and a sensitivity of 2674.7 μA·μM−1·cm−2. It has outstanding selectivity, satisfactory reproducibility and desired stability. It was used to quantify ATP in ATP-spiked 10% serum solutions.

Graphical abstract

Schematic presentation of the construction of the aptamer based electrode for voltammetric determination of ATP.


Adenosine triphosphate GO-PEDOT composite film Zwitterionic peptide Electrochemical aptasensor Electrodeposition Contact angle Antifouling ability Aptasensor Clinical analysis 



The work reported here was supported by the National Natural Science Foundation of China under Grant No.51672144, 51572137, 51502149, 51702181, the Natural Science Foundation of Shandong Province under Grant No. ZR2016EMB25, ZR2017PEM006, ZR2017BB013, the Higher Educational Science and Technology Program of Shandong Province under Grant No.J16LA10, J17KA014, the Application Foundation Research Program of Qingdao under Grant No. 15-9-1-28-jch, the Taishan Scholars Program of Shandong Province under No. ts201511034 and the Overseas Taishan Scholars Program. We express our grateful thanks to them for their financial support.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3211_MOESM1_ESM.docx (417 kb)
ESM 1 (DOCX 416 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Zhenjiang Li
    • 1
  • Jifang Yin
    • 2
  • Chenghai Gao
    • 2
  • Liying Sheng
    • 1
  • Alan Meng
    • 3
  1. 1.Key Laboratory of Polymer Material Advanced Manufacturing Technology of Shandong Provincial, College of Electromechanical Engineering, College of Sino-German Science and TechnologyQingdao University of Science and TechnologyQingdaoPeople’s Republic of China
  2. 2.College of Materials Science and EngineeringQingdao University of Science and TechnologyQingdaoPeople’s Republic of China
  3. 3.Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdaoPeople’s Republic of China

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