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Microchimica Acta

, 186:89 | Cite as

Blended gold/MnO2@BSA nanoparticles for fluorometric and magnetic resonance determination of ascorbic acid

  • Jiani Yu
  • Weitao Yang
  • Shige Xing
  • Jun Wang
  • Huanxing Han
  • Pengfei Zhang
  • Chenyang Xiang
  • Bingbo ZhangEmail author
Original Paper
  • 105 Downloads

Abstract

A fluorometric and magnetic resonance (MR) dual-modal detection scheme is presented for determination of ascorbic acid (AA). It is based on the use of a blended Au/MnO2@BSA mixture that was prepared via a biomimetic strategy, using bovine serum albumin (BSA) as the template at physiological temperature. The MnO2@BSA fraction (one part of the composite) is not susceptible to MR but can be degraded to MR-active compounds upon a redox reaction with even ultralow concentrations of AA. In parallel, the blended Au/MnO2@BSA recovers its fluorescence because MnO2@BSA acts as a quencher of the fluorescence of circumjacent Au@BSA (the other part of the composite). Fluorescence typically is measured at excitation/emission wavelengths of 470/625 nm. Leveraging on this redox reaction between MnO2 and AA, a dual-mode detection scheme for AA was developed. Both the fluorescence and the MR signal increase with the concentration of AA. The lowest limit for the detection of AA is 0.6 μM in the fluorometric mode and 0.4 μM in the MR mode. Analysis of AA-spiked serum samples showed that the recoveries obtained by either the fluorometric and MR mode can reach 94%. This is the first report of the use of blended nanoparticles with their inherent cross-validation regularity.

Graphical abstract

Schematic presentation of the biomimetic synthesis of blended Au/MnO2@BSA nanoprobes and fluorometric/MR cross-validation dual-modal detection of ascorbic acid.

Keywords

Biomimetic approach Ascorbic acid Dual-modal detection 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (81571742, 81871399, 81801823), Project funded by China Postdoctoral Science Foundation (1500229020), Shanghai Innovation Program (14ZZ039), Program for Outstanding Young Teachers in Tongji University, and the Fundamental Research Funds for the Central Universities. International Science & Technology Cooperation Program of China (2014DFA33010).

Compliance with ethical standards

The author(s) declare that they have no competing interests

Supplementary material

604_2018_3205_MOESM1_ESM.docx (4.1 mb)
ESM 1 (DOCX 4.13 mb)

References

  1. 1.
    Cheng HJ, Wang XY, Wei H (2015) Ratiometric electrochemical sensor for effective and reliable detection of ascorbic acid in living brains. Anal Chem 87(17):8889–8895CrossRefGoogle Scholar
  2. 2.
    Li LB, Wang C, Liu KY, Wang YH, Liu K, Lin YQ (2015) Hexagonal cobalt oxyhydroxide-carbon dots hybridized surface: high sensitive fluorescence turn-on probe for monitoring of ascorbic acid in rat brain following brain ischemia. Anal Chem 87(6):3404–3411CrossRefGoogle Scholar
  3. 3.
    Sönmez M, Türk G, Yüce A (2005) The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology 63:2063–2072CrossRefGoogle Scholar
  4. 4.
    Jfy F, Chin SF, Ng SM (2016) A unique "turn-on" fluorescence signalling strategy for highly specific detection of ascorbic acid using carbon dots as sensing probe. Biosens Bioelectron 85:844–853CrossRefGoogle Scholar
  5. 5.
    Dong YP, Gao TT, Chu XF, Chen J, Wang CM (2014) Flow injection-chemiluminescence determination of ascorbic acid based on luminol–ferricyanide–gold nanoparticles system. J Lumin 154:350–355CrossRefGoogle Scholar
  6. 6.
    Gao X, Yu P, Wang YX, Ohsaka TK, Ye JS, Mao LQ (2013) Microfluidic chip-based online electrochemical detecting system for continuous and simultaneous monitoring of ascorbate and Mg2+ in rat brain. Anal Chem 85(15):7599–7605CrossRefGoogle Scholar
  7. 7.
    Xiang L, Yu P, Hao J, Zhang M, Zhu L, Dai L, Mao L (2014) Vertically aligned carbon nanotube-sheathed carbon fibers as pristine microelectrodes for selective monitoring of ascorbate in vivo. Anal Chem 86(8):3909–3914CrossRefGoogle Scholar
  8. 8.
    Contat-Rodrigo L, Pérez-Fuster C, Lidón-Roger JV, Bonfiglio A, García-Breijo E (2017) Screen-printed organic electrochemical transistors for the detection of ascorbic acid in food. Org Electron 45:89–96CrossRefGoogle Scholar
  9. 9.
    Meng HM, Zhang XB, Yang C, Kuai H, Mao GJ, Gong L, Zhang W, Feng S, Chang J (2016) Efficient two-photon fluorescence nanoprobe for turn-on detection and imaging of ascorbic acid in living cells and tissues. Anal Chem 88(11):6057–6063CrossRefGoogle Scholar
  10. 10.
    Zhao P, He KY, Han YT, Zhang Z, Yu MZ, Wang HH, Huang Y, Nie Z, Yao SZ (2015) Near-infrared dual-emission quantum dots-gold nanoclusters nanohybrid via co-template synthesis for ratiometric fluorescent detection and bioimaging of ascorbic acid in vitro and in vivo. Anal Chem 87(19):9998–10005CrossRefGoogle Scholar
  11. 11.
    Han QX, Dong Z, Tang XL, Wang L, Ju ZH, Liu WS (2016) A ratiometric nanoprobe consisting of up-conversion nanoparticles functionalized with cobalt oxyhydroxide for detecting and imaging ascorbic acid. J Mater Chem B 5:167–172CrossRefGoogle Scholar
  12. 12.
    Li N, Li YH, Han YY, Pan W, Zhang TT, Tang B (2014) A highly selective and instantaneous nanoprobe for detection and imaging of ascorbic acid in living cells and in vivo. Anal Chem 86(6):3924–3930CrossRefGoogle Scholar
  13. 13.
    Li YX, Chen YT, Huang L, Ma L, Lin Q, Chen GN (2015) A fluorescence sensor based on ovalbumin-modified Au nanoclusters for sensitive detection of ascorbic acid. Anal Methods 7(10):4123–4129CrossRefGoogle Scholar
  14. 14.
    Chen YP, Xian Y, Wang Y, Zhang XQ, Cha RT, Sun JS, Jiang XY (2015) One-step detection of pathogens and viruses: combining magnetic relaxation switching and magnetic separation. ACS Nano 9(3):3184–3191CrossRefGoogle Scholar
  15. 15.
    Chen X, Xu Y, Li HR, Liu BY (2017) A nanoclay-based magnetic/fluorometric bimodal strategy for ascorbic acid detection. Sensors Actuators B Chem 246:344–351CrossRefGoogle Scholar
  16. 16.
    Xu Y, Chen X, Chai R, Xing CF, Li HR, Yin XB (2016) A magnetic/fluorometric bimodal sensor based on a carbon dots-MnO2 platform for glutathione detection. Nanoscale 8(27):13414–13421CrossRefGoogle Scholar
  17. 17.
    Yang WT, Guo WS, Chang J, Zhang BB (2016) Protein/peptide-templated biomimetic synthesis of inorganic nanoparticles for biomedical applications. J Mater Chem B 5(3):401–417CrossRefGoogle Scholar
  18. 18.
    Xing XH, Zhang BB, Wang XH, Liu FJ, Shi DL, Cheng YS (2015) An "imaging-biopsy" strategy for colorectal tumor reconfirmation by multipurpose paramagnetic quantum dots. Biomaterials 48:16–25CrossRefGoogle Scholar
  19. 19.
    Li D, Hua MH, Fang K, Liang R (2017) BSA directed-synthesis of biocompatible Fe3O4 nanoparticles for dual-modal T1 and T2 MR imaging in vivo. Anal Methods 9(21):3099–3104CrossRefGoogle Scholar
  20. 20.
    Wang XH, Xing XH, Zhang BB, Liu FJ, Cheng YH, Shi DL (2014) Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells. Int J Nanomedicine 9:1601–1615CrossRefGoogle Scholar
  21. 21.
    Zhang BB, Jin HT, Li Y, Chen BD, Liu SY, Shi DL (2012) Bioinspired synthesis of gadolinium-based hybrid nanoparticles as MRI blood pool contrast agents with high relaxivity. J Mater Chem 22(29):14494–14501CrossRefGoogle Scholar
  22. 22.
    Zhang J, Hao GY, Yao CF, Yu JN, Wang J, Yang WT, Hu CH, Zhang BB (2016) Albumin-mediated biomineralization of paramagnetic NIR Ag2S QDs for tiny tumor bimodal targeted imaging in vivo. ACS Appl Mater Interfaces 8(26):16612–16621CrossRefGoogle Scholar
  23. 23.
    Yang WT, Guo WS, Le WJ, Lv GX, Zhang FH, Shi L, Wang XL, Wang J, Wang S, Chang J (2016) Albumin-bioinspired Gd:CuS nanotheranostic agent for in vivo photoacoustic/magnetic resonance imaging-guided tumor-trgeted photothermal therapy. ACS Nano 10(11):10245–10257CrossRefGoogle Scholar
  24. 24.
    Xie JP, Zheng YG, Ying JY (2009) Protein-directed synthesis of highly fluorescent gold nanoclusters. J Am Chem Soc 131(3):888–889CrossRefGoogle Scholar
  25. 25.
    Guével XL, Hötzer B, Jung G, Hollemeyer K, Trouillet V, Schneider M (2011) Formation of fluorescent metal (Au, Ag) nanoclusters capped in bovine serum albumin followed by fluorescence and spectroscopy. J Phys Chem C 115(22):10955–10963CrossRefGoogle Scholar
  26. 26.
    Wang YL, Cui YY, Gao XY (2012) Bifunctional peptides that precisely biomineralize Au clusters and specifically stain cell nuclei. Chem Commun 48(6):871–873CrossRefGoogle Scholar
  27. 27.
    Luo YL (2007) Preparation of MnO2 nanoparticles by directly mixing potassium permanganate and polyelectrolyte aqueous solutions. Mater Lett 61(8):1893–1895CrossRefGoogle Scholar
  28. 28.
    Sun SK, Dong LX, Cao Y, Sun HR, Yan XP (2013) Fabrication of multifunctional Gd2O3/au hybrid nanoprobe via a one-step approach for near-infrared fluorescence and magnetic resonance multimodal imaging in vivo. Anal Chem 85(17):8436–8441CrossRefGoogle Scholar
  29. 29.
    Zhu WW, Dong ZL, Fu TT, Liu JJ, Chen Q, Li YG, Zhu R, Xu LG, Liu Z (2016) Modulation of hypoxia in solid tumor microenvironment with MnO2 nanoparticles to enhance photodynamic therapy. Adv Funct Mater 26(30):5490–5498CrossRefGoogle Scholar
  30. 30.
    Romo-Herrera JM, Alvarez-Puebla RA, Liz-Marzán LM (2011) Controlled assembly of plasmonic colloidal nanoparticle clusters. Nanoscale 3(4):1304–1315CrossRefGoogle Scholar
  31. 31.
    Sperling RA, Parak WJ (2010) Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles. Philos Trans 47(1):1333–1383CrossRefGoogle Scholar
  32. 32.
    Zheng Y, Feng G, Shang T, Wu W, Huang J, Sun D, Wang Y, Li Q (2016) Separation of biosynthesized gold nanoparticles by density gradient centrifugation. Sep Sci 52(2):951–957Google Scholar
  33. 33.
    Huang Y, Zhang Y, Yan ZY, Liao SH (2015) Assay of ceftazidime and cefepime based on fluorescence quenching of carbon quantum dots. Lumin 30(7):1133–1138CrossRefGoogle Scholar
  34. 34.
    Zhao ZH, Wang XM, Zhang ZJ, Zhang H, Liu HY, Zhu XL, Li H, Chi XQ, Yin ZY, Gao JH (2015) Real-time monitoring of arsenic trioxide release and delivery by activatable T(1) imaging. ACS Nano 12(2):546–546Google Scholar
  35. 35.
    Cen Y, Tang J, Kong XJ, Wu S, Yuan J, Yu RQ, Chu X (2015) A cobalt oxyhydroxide-modified upconversion nanosystem for sensitive fluorescence sensing of ascorbic acid in human plasma. Nanoscale 7(33):13951–13957CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Photomedicine, Shanghai Skin Disease Hospital, The Institute for Biomedical Engineering & Nano ScienceTongji University School of MedicineShanghaiPeople’s Republic of China
  2. 2.Chongqing University of EducationChongqingPeople’s Republic of China
  3. 3.Institute of Food SafetyChinese Academy of Inspection & QuarantineBeijingPeople’s Republic of China
  4. 4.Department of Pharmacy, Changzheng HospitalSecond Military Medical UniversityShanghaiPeople’s Republic of China

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