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Microchimica Acta

, 186:85 | Cite as

Multifunctional DNA nanocage with CdTe quantum dots for fluorescence detection of human 8-oxoG DNA glycosylase 1 and doxorubicin delivery to cancer cells

  • Guifen JieEmail author
  • Xiaoshan Gao
  • Junjun Ge
  • Chunli Li
Original Paper
  • 63 Downloads

Abstract

A multifunctional DNA nanocage containing CdTe quantum dots (QDs) was prepared. It was applied to the fluorometric detection of human 8-oxoG DNA glycosylase 1 (hOGG1) by exonuclease-assisted cycling amplification technique. When loaded with the cancer drug doxorubicin (Dox), the nanocage is also a versatile probe for fluorescence imaging of cancer cells, and drug delivery to them. The presence of hOGG1 leads to the division of DNA HP1 (containing 8-oxo-dG) and formation of DNA fragments 1 and 2. Then, HP2 is added to hybridize with DNA 1 and produced lots of trigger DNA (containing nucleolin aptamer) by Exo III-aided cycling amplification. The DNA nanocage was fabricated by linking the trigger DNA to multiple specific DNA strands, and the fluorescent CdTe QDs were further conjugated to the DNA nanocage for sensitive detection of hOGG1 activity. After Dox is incorporated into the DNA nanocage, the fluorescence of Dox is turned off. Once the DNA nanocage enters the MCF-7 cells, the Dox is released and its fluorescence (measured at excitation/emission wavelengths of 480/560 nm) is turned on. The DNA nanocage containing fluorescent QDs and Dox was successfully applied to the fluorometric detection of hOGG1, fluorescence imaging, and therapy of cancer cells, which has great promise in clinical application and treatment of cancer.

Graphical abstract

A multifunctional DNA nanocage containing CdTe quantum dots and acting as a signalling probe was prepared. It was applied to fluorometric determination of human 8-oxoG DNA glycosylase 1 using cycling amplification technique. It also enables drug delivery to cancer cells if loaded with doxorubicin.

Keywords

DNA nanocage CdTe quantum dot Cycling amplification Fluorescence imaging Drug delivery 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No.21575072), and the Key Research and Development Plan of Shandong Province (2016GGX102021).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3199_MOESM1_ESM.doc (846 kb)
ESM 1 (DOC 846 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOEQingdao University of Science and TechnologyQingdaoPeople’s Republic of China
  2. 2.Shandong Key Laboratory of Biochemical AnalysisQingdao University of Science and TechnologyQingdaoPeople’s Republic of China
  3. 3.Key Laboratory of Analytical Chemistry for Life Science in Universities of ShandongQingdao University of Science and TechnologyQingdaoPeople’s Republic of China

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