Colorimetric and visual mercury(II) assay based on target-induced cyclic enzymatic amplification, thymine-Hg(II)-thymine interaction, and aggregation of gold nanoparticles
- 45 Downloads
A colorimetric biosensor and visual test is described for the determination of mercury(II). It relies on the specific thymine-Hg(II)-thymine (T-Hg2+-T) interaction which induces a cyclic amplification process (caused by the enzyme exonuclease III) and the aggregation of gold nanoparticles. These results in a color change from red to violet. Under optimized conditions, this colorimetric assay (best performed at 524 nm) has a detection limit as low as 0.9 nM with a detection range over 4 orders of magnitude (from 1 nM to 10 μM).
KeywordsMercury ion T-Hg2+-T Exonuclease III AuNPs Visual detection UV-vis absorption
This work was supported by National Natural Science Foundation of China (31471644), the Primary Research & Development Plan of Shandong Province (2017GSF220009). University of Jinan Scientific Research Fund (Youth Project XKY1421) and Doctoral Fundation of University of Jinan (XBS1431).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 5.Office of Water (2001) Mercury update: impact on fish advisories, EPA fact sheet EPA-823-F-01-011. U.S. In: Environmental Protection Agency. D. C, WashingtonGoogle Scholar
- 6.Zenko Y, Masao T (1977) Indirect determination of submicrogram amounts of sulfide by flameless atomic absorption spectrometry of mercury. Microchim Acta 67(5–6):459–468Google Scholar
- 18.Hong MQ, Zeng BH, Li MY, Xu XQ, Chen GN (2018) An ultrasensitive conformation-dependent colorimetric probe for the detection of mercury(II) using exonuclease III-assisted target recycling and gold nanoparticles. Microchim Acta 185(72). https://doi.org/10.1007/s00604-017-2536-1