Microchimica Acta

, 186:118 | Cite as

Intrinsic peroxidase-like activity of Cu2ZnSn(SxSe1-x)4 nanocrystals, and their application to the colorimetric detection of H2O2

  • Peng Ju
  • Jinfeng Ding
  • Bing Wang
  • Wen Li
  • Fenghua Jiang
  • Xiuxun Han
  • Chengjun SunEmail author
  • Chi WuEmail author
Original Paper


Nanocrystals (NCs) of type Cu2ZnSn(SxSe1-x)4 (CZTSSe) were prepared via a solvothermal approach. They are shown to be highly efficient peroxidase (POx) mimics for colorimetric detection of H2O2. By varying the molar ratio of S and Se during preparation, the NCs showed different crystal structures, morphologies, surface properties, and POx-like activities. Among them, the type CZTSSe-0.25 NCs exhibit the strongest POx-like activities towards the catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine in the presence of H2O2 to generate a blue product. The enhanced activity is attributed to its more negative potential and larger specific surface of the NCs. Based on these findings, a rapid and ultrasensitive method was developed for the visual and colorimetric determination of H2O2. The method is selective, and the NCs are reusable and long-term stable. The detection limit of H2O2 is 50 nM. Kinetic and active species trapping experiments were performed to elucidate the POx-like mechanism of the NCs.

Graphical abstract

Schematic presentation of the process of Cu2ZnSn(SxSe1-x)4 nanocrystals catalyzing the oxidation of peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2 to induce a typical blue color reaction, which can be applied in colorimetric detection of H2O2.


Nanomaterials Peroxidase mimetic Catalytic activity Optical determination Sensor Kinetics XRD TEM TMB Hydrogen peroxide 



This work was supported by National Natural Science Foundation of China (51702328), Key Lab of Marine Bioactive Substance and Modern Analytical Technique, SOA (MBSMAT-2016-05), Natural Science Foundation of Shandong Province, China (ZR2017BD002), China Postdoctoral Science Foundation Funded Project (2017 M622179 and 2018 T110681), CAS “Light of West China” Program, Open Fund of Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao) (LMEES201802), National Natural Science Foundation of China (41776177), Open Foundation of Pilot National Laboratory for Marine Science and Technology (Qingdao) (QNLM2016ORP0410), and The Aoshan Scientific and Technological Innovation Project Financially Supported by Pilot National Laboratory for Marine Science and Technology (Qingdao) (2016ASKJ14). C.J. Sun would also like to thank support from Taishan Scholar and the Ministry of Human Resources and Social Security of China.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3185_MOESM1_ESM.pdf (1.7 mb)
ESM 1 (PDF 1.71 mb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Marine Science and TechnologyShandong UniversityQingdaoPeople’s Republic of China
  2. 2.Key Laboratory of Marine Bioactive Substances and Analytical Technology, Marine Ecology Center, The First Institute of OceanographyState Oceanic Administration (SOA)QingdaoPeople’s Republic of China
  3. 3.Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengduChina
  4. 4.Institute of Semiconductor MaterialsJiangxi University of Science and TechnologyGanzhouPeople’s Republic of China
  5. 5.Laboratory of Marine Drugs and BioproductsPilot National Laboratory for Marine Science and Technology (Qingdao)QingdaoPeople’s Republic of China

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