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Microchimica Acta

, 186:52 | Cite as

Determination of 17β-estradiol by surface-enhanced Raman spectroscopy merged with hybridization chain reaction amplification on Au@Ag core-shell nanoparticles

  • Li Yao
  • Yulin Li
  • Kewen Cheng
  • Daodong Pan
  • Jianguo Xu
  • Wei Chen
Original Paper
  • 52 Downloads

Abstract

The authors describe an aptamer-based assay for 17β-estradiol. It relies on the combined use of surface enhanced Raman scattering (SERS) and hybridization chain reaction (HCR). The aptamer against 17β-estradiol is applied as the recognition probes, and this results in excellent specificity. Specific recognition of target 17β-estradiol induce the freedom of DNA 2, which will open the stem-loop structure of probe 1 on the Au@Ag and form the partial dsDNA structure. With the nicking enzyme, the partial dsDNA will be hydrolyzed and the reside ssDNA on Au@Ag will form a small stem-loop structure. With the help of the other probe 2 modified Au@Ag and pre-immobilized probe 3 on the well of the microplate, an enzyme-free HCR can occur and tremendous Au@Ag can be assembled along the formed dsDNA in HCR, which can act as the excellent substrate for Raman measurement and greatly amplify the Raman signal of R6G on the Au@Ag. Afterwards, the key factor, ratio between probe 2-Au@Ag (P2) and probe1-Au@Ag (P1), affects the detection sensitivity is systematically optimized for the best sensing performance. The SERS signal of R6G, best measured at 1651 cm−1, increases linearly in the wide range from 1 pM to 10 nM. The detection limit can be as low as 0.1 pM.

Graphical abstract

Schematic presentation of an aptamer-based surface enhanced Raman scattering method for accurate detection of 17β-estradiol, which is integrated with hybridization chain reaction for signal amplification and sensitivity improvement.

Keywords

Estrogen Aptamer SERS Hybridization chain reaction Signal amplification Gold nanoparticle Environment monitoring Food safety 

Notes

Acknowledgements

This work is financially supported by the grant of 2017YFF0208600, the NSFC Grant of 21475030, Open Foundation of Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization of EWPL201701, the China Agriculture Research System-48 (CARS-48), Anhui Provincial Modern Argo-industry Tech. Research System (NYCYTX-2016-84) and the Fundamental Research Fund for central university (Grants No. 2017HGPA0162, JZ2018HGTA0205, PA2017GDQT0018).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3114_MOESM1_ESM.doc (154 kb)
ESM 1 (DOC 154 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Food Science and Biological Engineering, Engineering Research Center of Bio-process, MOEHefei University of TechnologyHefeiChina
  2. 2.Hubei Key Laboratory of Edible Wild Plants Conservation and UtilizationHubei Normal UniversityHuangshiChina
  3. 3.Faculty of Marine ScienceNingbo UniversityNingboChina

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