Aluminum(III) triggered aggregation-induced emission of glutathione-capped copper nanoclusters as a fluorescent probe for creatinine
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Glutathione-capped copper nanoclusters (CuNCs) are presented that display aggregation-induced emission (AIE). This feature was exploited for selective and sensitive quantification of creatinine (CRN) which is an important diagnostic parameter. In the presence of Al3+ ions, such CuNCs rapidly aggregate, and this induces enhanced a red emission. The AIE nature of CuNCs was proven via TEM and fluorimetry. On addition of CRN, the coordination between CRN and Al3+ ions led to the quenching of fluorescence due to weakening the AIE. The best fluorescence intensity was measured at excitation/emission peaks of 360/585 nm. Quenched fluorescence intensity showed a linear dependence on the concentrations of CRN in the range of 2.5–34 μgL−1 with a detection limit of 0.63 μgL−1. The sensing mechanism of probe for CRN detection is discussed. The probe was applied to the determination of CRN in spiked human serum samples and gave satisfactory results.
KeywordsFluorescent nanomaterials Fluorometry Metal nanoclusters Nanosensor Nanoprobe Paired t-test Renal biomarker Real sample analysis Serum analysis
Authors thank the University of Tabriz for the support provided. Roghayeh Jalili as a postdoc researcher gratefully acknowledges use of the services and facilities of the University of Tabriz.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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