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Microchimica Acta

, 185:559 | Cite as

Human serum albumin templated MnO2 nanosheets are oxidase mimics for colorimetric determination of hydrogen peroxide and for enzymatic determination of glucose

  • Jia Ge
  • Ke Xing
  • Xin Geng
  • Ya-Lei Hu
  • Xue-Ping Shen
  • Lin Zhang
  • Zhao-Hui Li
Original Paper
  • 268 Downloads

Abstract

This paper reports on a colorimetric assay for H2O2 and glucose. It is based on the use of human serum albumin-templated MnO2 nanosheets that possess oxidase-like activity. They are capable of oxidizing 3,3',5,5'-tetramethylbenzidine (TMB) with oxygen to give a blue product (oxTMB) with an absorbance maximum at 652 nm. When H2O2 is introduced, the MnO2 nanosheets are reduced to Mn(II) ions, and this inhibits the formation of oxTMB. Based on these findings, a colorimetric assay was established for H2O2 that has a 0.56 μM detection limit. If glucose is oxidized by glucose oxidase under formation of H2O2, the nanosheets can be used to quantify H2O2 and thereby to sense glucose. Response is linear in the 0.5 μM to 50 μM glucose concentration range, and the detection limit is 0.32 μM. The method was applied to the determination of glucose in spiked serum samples and gave satisficatory results.

Graphical abstract

Human serum albumin (HSA) is used as a template for the synthesis of MnO2 nanosheet. These possess oxidase mimicking activity. H2O2 can reduce the nanosheets. The effect is exploited in colorimetric assays for H2O2 and glucose using tetramethylbenzidine (TMB) as a chromogenic substrate.

Keywords

HSA Optical sensor Colorimetric assay Glucose oxidase 3,3 ́,5,5 ́-Tetramethylbenzidine Human serum 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (21505122), and the Outstanding Young Talent Research Fund of Zhengzhou University (1521316003), the Foundation for University Key Teacher by Henan Province (2017GGJS007), and the Key Scientific Research Project in Universities of Henan Province (19A150048).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3099_MOESM1_ESM.doc (1.3 mb)
ESM 1 (DOC 1330 kb)

References

  1. 1.
    Liu JW, Luo Y, Wang YM, Duan LY, Jiang JH, Yu RQ (2016) Graphitic carbon nitride nanosheets-based ratiometric fluorescent probe for highly sensitive detection of H2O2 and glucose. ACS Appl Mater Interfaces 8:33439–33445CrossRefGoogle Scholar
  2. 2.
    Xu Y, Pehrsson PE, Chen LW, Zhang R, Zhao W (2007) Double-stranded DNA single-walled carbon nanotube hybrids for optical hydrogen peroxide and glucose sensing. J Phys Chem C 111:8638–8643CrossRefGoogle Scholar
  3. 3.
    Lu J, Bu RF, Sun ZL, Lu QS, Jin H, Wang Y, Wang SH, Li L, Xie ZL, Yang BQ (2011) Comparable efficacy of self-monitoring of quantitative urine glucose with self-monitoring of blood glucose on glycaemic control in non-insulin-treated type 2 diabetes. Diabetes Res Clin Pr 93:179–186CrossRefGoogle Scholar
  4. 4.
    Zhu XL, Huang J, Liu JW, Zhang H, Jiang JH, Yu RQ (2017) A dual enzyme–inorganic hybrid nanoflower incorporated microfluidic paper-based analytic device (μPAD) biosensor for sensitive visualized detection of glucose. Nanoscale 9:5658–5663CrossRefGoogle Scholar
  5. 5.
    Chen S, Hai X, Chen XW, Wang JH (2014) In situ growth of silver nanoparticles on graphene quantum dots for ultrasensitive colorimetric detection of H2O2 and glucose. Chem Commun 86:6689–6694Google Scholar
  6. 6.
    Zhao Y, Wang Y, Zhang XB, Kong RM, Xia L, Qu FL (2016) Cascade enzymatic catalysis in poly(acrylic acid) brushes-nanospherical silica for glucose detection. Talanta 155:265–271CrossRefGoogle Scholar
  7. 7.
    Chen Q, Liu ML, Zhao JN, Peng X, Chen XJ, Mi NX, Yin BD, Li HT, Zhang YY, Yao SZ (2014) Water-dispersible silicon dots as a peroxidase mimetic for the highly-sensitive colorimetric detection of glucose. Chem Commun 50:6771–6774CrossRefGoogle Scholar
  8. 8.
    Li L, Gao FF, Ye J, Chen ZZ, Li QL, Gao W, Ji LF, Zhang RR, Tang B (2013) FRET-based biofriendly Apo-GOx-modified gold nanoprobe for specific and sensitive glucose sensing and cellular imaging. Anal Chem 85:9721–9727CrossRefGoogle Scholar
  9. 9.
    Wu P, He Y, Wang HF, Yan XP (2010) Conjugation of glucose oxidase onto Mn-doped ZnS quantum dots for phosphorescent sensing of glucose in biological fluids. Anal Chem 82:1427–1433CrossRefGoogle Scholar
  10. 10.
    Wang HB, Chen Y, Li N, Liu YM (2017) A fluorescent glucose bioassay based on the hydrogen peroxide-induced decomposition of a quencher system composed of MnO2 nanosheets and copper nanoclusters. Microchim Acta 184:515–523CrossRefGoogle Scholar
  11. 11.
    Song J, Xu L, Zhou CY, Xing RQ, Dai QL, Liu DL, Song HW (2013) Synthesis of graphene oxide based CuO nanoparticles composite electrode for highly enhanced nonenzymatic glucose detection. ACS Appl Mater Inter 5:12928–12934CrossRefGoogle Scholar
  12. 12.
    Lan D, Li BX, Zhang ZJ (2008) Chemiluminescence flow biosensor for glucose based on gold nanoparticle-enhanced activities of glucose oxidase and horseradish peroxidase. Biosens Bioelectron 24:934–938CrossRefGoogle Scholar
  13. 13.
    Shi H, Li D, Xun FZ, He XX, Wang KM, Ye XS, Tang JL, He CM (2014) A label-free activatable aptamer probe for colorimetric detection of cancer cells based on binding-triggered in situ catalysis of split DNAzyme. Analyst 139:4181–4184CrossRefGoogle Scholar
  14. 14.
    Ye XS, Shi H, He XX, Wang KM, He DG, Yan LA, Xun FZ, Lei YL, Tang JL, Yu YR (2015) Iodide-responsive cu−au nanoparticle-based colorimetric platform for ultrasensitive detection of target cancer cells. Anal Chem 87:7141–7147CrossRefGoogle Scholar
  15. 15.
    Kotov NA (2010) Inorganic nanoparticles as protein mimics. Science 330:188–189CrossRefGoogle Scholar
  16. 16.
    Dong YL, Zhang HG, Rahman ZU, Su L, Chen XJ, Hu J, Chen XG (2012) Graphene oxide-Fe3O4 magnetic nanocomposites with peroxidase-like activity for colorimetric detection of glucose. Nanoscale 4:3969–3976CrossRefGoogle Scholar
  17. 17.
    Yang WS, Hao JH, Zhang Z, Zhang BL (2015) PB@Co3O4 nanoparticles as both oxidase and peroxidase mimics and their application for colorimetric detection of glutathione. New J Chem 39:8802–8806CrossRefGoogle Scholar
  18. 18.
    Gao ZQ, Xu MD, Hou L, Chen GN, Tang DP (2013) Irregular-shaped platinum nanoparticles as peroxidase mimics for highly efficient colorimetric immunoassay. Anal Chim Acta 776:79–86CrossRefGoogle Scholar
  19. 19.
    He WW, Liu Y, Yuan JS, Yin JJ, Wu XC, Hu XN, Zhang K, Liu JB, Chen CY, Ji YL, Guo YT (2011) Au@Pt nanostructures as oxidase and peroxidase mimetics for use in immunoassays. Biomaterials 32:1139–1147CrossRefGoogle Scholar
  20. 20.
    Wang HB, Yang L, Bai HY, Liu YM (2018) DNA-templated au nanoclusters and MnO2 sheets: a label-free and universal fluorescence biosensing platform. Sensor Actuat B-Chem 259:204–210CrossRefGoogle Scholar
  21. 21.
    Yang HG, Xiong YH, Zhang P, Su LJ, Ye FG (2015) Colorimetric detection of mercury ions using MnO2 nanorods as enzyme mimics. Anal Methods 7:4596–4601CrossRefGoogle Scholar
  22. 22.
    Wei WF, Cui XW, Chen WX, Ivey DG (2011) Manganese oxide-based materials as electrochemical supercapacitor electrodes. Chem Soc Rev 40:1697–1721CrossRefGoogle Scholar
  23. 23.
    Safdar M, Wani OM, Jänis J (2015) Manganese oxide-based chemically powered micromotors. ACS Appl Mater Inter 7:25580–25585CrossRefGoogle Scholar
  24. 24.
    Tan QQ, Zhang RR, Kong RM, Kong WS, Zhao WZ, Qu FL (2018) Detection of glutathione based on MnO2 nanosheet-gated mesoporous silica nanoparticles and target induced release of glucose measured with a portable glucose meter. Microchim Acta 185:44CrossRefGoogle Scholar
  25. 25.
    Wang HB, Chen Y, Li Y, Liu YM (2016) A sensitive fluorescence sensor for glutathione detection based on MnO2 nanosheets–copper nanoclusters composites. RSC Adv 6:79526–79532CrossRefGoogle Scholar
  26. 26.
    Qu FL, Pei HM, Kong RM, Zhu SY, Xia L (2017) Novel turn-on fluorescent detection of alkaline phosphatase based on green synthesized carbon dots and MnO2 nanosheets. Talanta 165:136–142CrossRefGoogle Scholar
  27. 27.
    Song ML, Liu T, Shi CR, Zhang XZ, Chen XY (2016) Bioconjugated manganese dioxide nanoparticles enhance chemotherapy response by priming tumor-associated macrophages toward M1-like phenotype and attenuating tumor hypoxia. ACS Nano 10:633–647CrossRefGoogle Scholar
  28. 28.
    Jones MR, Osberg KD, Macfarlane RJ, Langille MR, Mirkin CA (2011) Templated techniques for the synthesis and assembly of plasmonic nanostructures. Chem Rev 111:3736–3827CrossRefGoogle Scholar
  29. 29.
    Xie JP, Zheng YG, Ying JY (2009) Protein-directedsynthesis of highly fluorescent gold nanoclusters. J Am Chem Soc 131:888–889CrossRefGoogle Scholar
  30. 30.
    Li D, Qiao ZZ, Yu YR, Tang JL, He XX, Shi H, Ye XS, Lei YL, Wang KM (2018) In situ fluorescence activation of DNA–silver nanoclusters as a label-free and general strategy for cell nucleus imaging. Chem Commun 54:1089–1092CrossRefGoogle Scholar
  31. 31.
    Luo L, Xu FZ, Shi H, He XX, Qing TP, Lei YL, Tang JL, He DG, Wang KM (2017) Label-free and sensitive assay for deoxyribonuclease I activity based on enzymatically-polymerized superlong poly(thymine)-hosted fluorescent copper nanoparticles. Talanta 169:57–63CrossRefGoogle Scholar
  32. 32.
    Xu FZ, Shi H, He XX, Wang KM, He DG, Guo QP, Qing ZH, Yan LA, Ye XS, Li D, Tang JL (2014) Concatemeric dsDNA-templated copper nanoparticles strategy with improved sensitivity and stability based on rolling circle replication and its application in microRNA detection. Anal Chem 86:6976–6982CrossRefGoogle Scholar
  33. 33.
    Dutta AK, Maji SK, Srivastava DN, Anup M, Biswas P, Paul P, Adhikary B (2012) Peroxidase-like activity and amperometric sensing of hydrogen peroxide by Fe2O3 and Prussian blue-modified Fe2O3 nanoparticles. ACS Appl Mater Inter 4:1919–1927CrossRefGoogle Scholar
  34. 34.
    Fan YW, Huang YM (2012) The effective peroxidase-like activity of chitosan-functionalized CoFe2O4 nanoparticles for chemiluminescence sensing of hydrogen peroxide and glucose. Analyst 137:1225–1231CrossRefGoogle Scholar
  35. 35.
    Lv CJ, Di WH, Liu ZH, Zheng KZ, Qin WP (2014) Luminescent CePO4:Tb colloids for H2O2 and glucose sensing. Analyst 139:4547–4555CrossRefGoogle Scholar
  36. 36.
    Wei H, Wang EK (2008) Fe3O4 magnetic nanoparticles as peroxidase mimetics and ttheir applications in H2O2 and glucose detection. Anal Chem 80:2250–2254CrossRefGoogle Scholar
  37. 37.
    Hu LZ, Yuan YL, Zhang L, Zhao JM, Majeed S, Xu GB (2013) Copper nanoclusters as peroxidase mimetics and their applications to H2O2 and glucose detection. Anal Chim Acta 762:83–86CrossRefGoogle Scholar
  38. 38.
    Jv Y, Li BX, Cao R (2010) Positively-charged gold nanoparticles as peroxidase mimic and their application in hydrogen peroxide and glucose detection. Chem Commun 46:8017–8019CrossRefGoogle Scholar
  39. 39.
    Song YJ, Qu KG, Zhao C, Ren JS, Qu XG (2010) Graphene oxide: intrinsic peroxidase catalytic activity and its application to glucose detection. Adv Mater 22:2206–2210CrossRefGoogle Scholar
  40. 40.
    Su L, Feng J, Zhou X, Ren C, Li H, Chen X (2012) Colorimetric detection of urine glucose based ZnFe2O4 magnetic nanoparticles. Anal Chem 84:5753–5758CrossRefGoogle Scholar
  41. 41.
    Choleva TG, Gatselou VA, Tsogas GZ, Giokas DL (2018) Intrinsic peroxidase-like activity of rhodium nanoparticles, and their application to the colorimetric determination of hydrogen peroxide and glucose. Microchim Acta 185:22CrossRefGoogle Scholar
  42. 42.
    Huang LJ, Zhu WX, Zhang WT, Chen K, Wang J, Wang R, Yang QF, Hu N, Suo YR, Wang JL (2018) Layered vanadium(IV) disulfide nanosheets as a peroxidase-like nanozyme for colorimetric detection of glucose. Microchim Acta 185:7CrossRefGoogle Scholar
  43. 43.
    Tran HV, Nguyen TV, Nguyen ND, Piro B, Huynh CD (2018) A nanocomposite prepared from FeOOH and N-doped carbon nanosheets as a peroxidase mimic, and its application to enzymatic sensing of glucose in human urine. Microchim Acta 185:270CrossRefGoogle Scholar
  44. 44.
    Han QX, Mou ZL, Wang HH, Tang XL, Dong Z, Wang L, Dong X, Liu WS (2016) Highly selective and sensitive one- and two-photon ratiometric fluorescent probe for intracellular hydrogen polysulfide sensing. Anal Chem 88:7206–7212CrossRefGoogle Scholar
  45. 45.
    Cen Y, Tang J, Kong XJ, Wu S, Yuan J, Yu RQ, Chu X (2015) A cobalt oxyhydroxide-modified upconversion nanosystem for sensitive fluorescence sensing of ascorbic acid in human plasma. Nanoscale 7:13951–13957CrossRefGoogle Scholar
  46. 46.
    Hu QH, Yu CM, Xia XT, Zeng F, Wu SZ (2016) A fluorescent probe for simultaneous discrimination of GSH and Cys/Hcy in human serum samples via distinctly-separated emissions with independent excitations, Biosens. Bioelectron 81:341–348CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Chemistry and Molecular EngineeringZhengzhou UniversityZhengzhouPeople’s Republic of China

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