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Microchimica Acta

, 185:558 | Cite as

A glassy carbon electrode with electrodeposited silver nanoparticles for aptamer based voltammetric determination of trinitrotoluene using riboflavin as a redox probe

  • Mahmoud RoushaniEmail author
  • Faezeh Shahdost-fardEmail author
Original Paper

Abstract

An electrochemical nanoaptasensor is described that is based on the use of a glassy carbon electrode (GCE) modified with electrodeposited silver nanoparticles (AgNPs). An aptamer (Apt) against trinitrotoluene (TNT) was then immobilized on the AgNPs. The addition of TNT to the modified GCE leads to decrease in peak current (typically measured at a potential of −0.45 V vs. Ag/AgCl) of riboflavin which acts as an electrochemical probe. Even small changes in the surface (as induced by binding of Apt to TNT) alter the interfacial properties. As a result, the LOD is lowered to 33 aM, and the dynamic range extends from 0.1 fM to 10 μM without sacrificing specificity.

Graphical abstract

Schematic presentation of a nanoaptasensor which is based on a glassy carbon electrode (GCE) modified with electrodeposited silver nanoparticles (AgNPs) and aptamer (Apt). It was applied to the detection of 2,4,6-trinitrotoluene (TNT) with the help of riboflavin (RF) as a redox probe.

Keywords

TNT Aptasensor AgNPs Electrodeposition Aptasensig assay 

Notes

Acknowledgements

This work was funded by Ilam University and the financial support of this University is gratefully acknowledged.

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3098_MOESM1_ESM.docx (468 kb)
ESM 1 (DOCX 467 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of ChemistryIlam UniversityIlamIran

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