Microchimica Acta

, 185:551 | Cite as

An electrochemical chlorpyrifos aptasensor based on the use of a glassy carbon electrode modified with an electropolymerized aptamer-imprinted polymer and gold nanorods

  • Mahmoud RoushaniEmail author
  • Azizollah Nezhadali
  • Zeynab Jalilian
Original Paper


A highly selective and sensitive aptasensor is described for voltammetric determination of the pesticide chlorpyrifos (CPS). The sensor was constructed by modifying a glassy carbon electrode (GCE) with gold nanorods and a polymer that was molecularly imprinted with an aptamer against CPS. This results in double specific recognition. Under optimal conditions and a working potential as low as 0.22 V (vs. Ag/AgCl), the nanotools has a dynamic range that covers the 1.0 fM - 0.4 pM CPS concentration range, and the detection limit is 0.35 fM. This is lower than any of the previously reported methods. This MIP-aptasensor is selective over structural analogs, stable, and adequately reproducible. It was successfully applied to the determination of CPS in spiked food samples.

Graphical abstract

Impedimetric detection of Chlorpyrifos by using a Fe(CN)63−/4- probe based on double recognition of aptamer-molecular imprinted polymer onto a glassy carbon electrode modified with gold nanorod nanocomposite. The incubation with Chlorpyrifos lead to an increase of electron transfer resistance.


o-Dihydroxybenzene o-Phenylenediamine Hexacyanoferrate Nanohybrid Differential pulse voltammetry Electrochemical impedance spectroscopy Pesticide 


Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3083_MOESM1_ESM.docx (143 kb)
ESM 1 (DOCX 142 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Mahmoud Roushani
    • 1
    Email author
  • Azizollah Nezhadali
    • 2
  • Zeynab Jalilian
    • 2
  1. 1.Department of ChemistryIlam UniversityIlamIran
  2. 2.Department of ChemistryPayame Noor UniversityTehranIran

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