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Microchimica Acta

, 185:505 | Cite as

An amino-functionalized mesoporous silica (KIT-6) as a sorbent for dispersive and ultrasonication-assisted micro solid phase extraction of hippuric acid and methylhippuric acid, two biomarkers for toluene and xylene exposure

  • Mohammad Behbahani
  • Saman Bagheri
  • Fariborz Omidi
  • Mostafa Mohammadpour Amini
Original Paper
  • 86 Downloads

Abstract

The authors described a new application of amino-functionalized KIT-6 for dispersive ultrasonication-assisted micro solid phase extraction of hippuric acid (HA) and methyl hippuric acid (MHA) from human urine and water samples. In the first step, an amino-functionalized mesoporous silica of type KIT-6 was synthesized. It was characterized by field emission scanning electron microscopy, Fourier transform infrared spectrometry, nitrogen adsorption-desorption analysis, thermogravimetry and X-ray diffraction. Following sorption and desorption with 1.0 mL methanol/NH4OH (1%; v/v), HA and MHA were quantified by HPLC with UV detection. Various important parameters were optimized by Box-Behnken design. Under optimized conditions, The limit of detections (LOD) were calculated by a signal-to-noise ratio of 3, which were 0.5 μg L−1 and 0.2 μg L-1 for HA and MHA, respectively, and the calibration plot is linear in the 1–1000 μg L−1 concentration range. Only small matrix effects were found. The method was successfully implemented for the sensitive determination of HA and MHA in (spiked) human urine samples.

Graphical abstract

Schematic of a sorbent for dispersive micro solid phase extraction coupled with ultrasonic power. It consists of amino functionalized KIT-6 and was used for the simultaneous preconcentration and determination of low levels of hippuric and methyl hippuric acid in urine and water samples.

Keywords

Urine sample High performance liquid chromatography Box-Behnken design Simultaneous detection Metabolites Occupational health Surface functionalization 

Notes

Acknowledgements

We gratefully acknowledge Iranian National Science Foundation (INSF).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3038_MOESM1_ESM.docx (258 kb)
ESM 1 (DOCX 258 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Mohammad Behbahani
    • 1
  • Saman Bagheri
    • 2
  • Fariborz Omidi
    • 3
  • Mostafa Mohammadpour Amini
    • 2
  1. 1.Faculty of EngineeringShohadaye Hoveizeh University of TechnologySusangerdIran
  2. 2.Department of ChemistryShahid Beheshti UniversityTehranIran
  3. 3.Research Center for Environmental Determinants of Health (RCEDH)Kermanshah University of Medical SciencesKermanshahIran

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