A needle-like reusable surface-enhanced Raman scattering substrate, and its application to the determination of acetamiprid by combining SERS and thin-layer chromatography
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A micro surface-enhanced Raman scattering (SERS) substrate has been fabricated by electrochemical deposition of dendrite-like gold on carbon fiber needles (Au-CFNs). Scanning electron microscopy and energy dispersive spectroscopy were used to confirm the presence of the gold nanostructure on the CFNs. This substrate has a Raman scattering enhancement factor as high as 3.3 × 10^7 when using rhodamine 6G as the reporter molecule. The high SERS sensitivity is attributed to the massive hotspots on gold bulges that enhance the local surface plasmon resonance. The Au-CFN substrate was reproduced 10 times after electrochemically wiping off the analytes from the needle-like electrode. The substrate has attractive features such as convenient sampling, low sample dosage, and minimal invasion. It was applied, in combination with thin-layer chromatography, for the determination of acetamiprid on vegetables. The result was more accurate because the sample information of both the surface and the bulk can be obtained at the same time after inserting the tip of this needle substrate into the TLC plate. The limit of detection for acetamiprid is 0.05 μg⋅mL-1 and the linear range is 0.1–10 μg⋅mL-1.
KeywordsAcetamiprid Micro substrate Carbon fiber needles Electrochemical deposition Dendrite-like gold
This work was supported by Science and Technology Commission of Shanghai Municipality (No.17142202600), Fundamental Research Funds for the Central Universities (No. 222201714047), Natural Science Foundation of Shanghai(18ZR1408100)and Scientific research project of MPS (2016JSYJC31, 2016JSYJB34).
Compliance with ethical standards
Yan Kang has received research grants from Science and Technology Commission of Shanghai Municipality. The author(s) declare that they have no competing interests.
This article does not contain any studies with human participants or animals performed by any of the authors.
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