Enzymatic determination of uric acid using water-soluble CuInS/ZnS quantum dots as a fluorescent probe
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Glutathione-capped water-soluble CuInS/ZnS quantum dots (QDs) were prepared by a microwave-assisted method. Their fluorescence, with excitation/emission peaks at 380/570 nm, is found to be quenched by hydrogen peroxide (H2O2) that is produced by the uricase catalyzed oxidation of uric acid (UA) and oxygen. The findings are used in a quenchometric method for the determination of UA. The effects of different ligands on the QDs, of pH value, buffers, enzyme ratio and reaction time were optimized. The detection limit for UA is 50 nM which is lower than other QD-based method, and the detection ranges extends from 0.25–4.0 μM. The assay is simple and sensitive, and no further modification of the QDs is required.
KeywordsMicrowave-based synthesis Nanoparticle synthesis Fluorescent probe Uric acid determination Human urine and serum
This work was supported by Special-funded Programme on national natural Science Foundation of China (Nos. 21405057 and 21207047), Science and Technology Developing Foundation of Jilin Province of China (Nos. 201701011106JC, 20160623025TC and 20160204010GX), Open Funds of the State Key Laboratory of Electroanalytical Chemistry (No. SKLEAC201704), and Graduate Innovation Fund of Jilin University (No. 2017152).
Compliance with ethical standards
The author(s) declare that they have no competing interests.
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