Microchimica Acta

, 185:486 | Cite as

Electrochemical determination of dopamine and uric acid using a glassy carbon electrode modified with a composite consisting of a Co(II)-based metalorganic framework (ZIF-67) and graphene oxide

  • Jing TangEmail author
  • Sixun Jiang
  • Yu Liu
  • Shengbiao Zheng
  • Lei Bai
  • Jiahao Guo
  • Jianfei WangEmail author
Original Paper


A composite was prepared from a Co(II)-based zeolitic imidazolate framework (ZIF-67) and graphene oxide (GO) by an in situ growth method. The material was electrodeposited on a glassy carbon electrode (GCE). The modified GCE was used for the simultaneous voltammetric determination of dopamine (DA) and uric acid (UA), typically at working potentials of 0.11 and 0.25 V (vs. SCE). The morphology and structure of the nanocomposite were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction. The modified electrode exhibits excellent electroanalytical performance for DA and UA owing to the synergistic effect of the high electrical conductivity of GO and the porosity of ZIF-67. By applying differential pulse voltammetry, a linear response is found for DA in the 0.2 to 80 μM concentration range, and for UA between 0.8 and 200 μM, with detection limits of 50 and 100 nM (at S/N = 3), respectively. Further studies were performed on the effect of potential interferents, and on electrode stability and reproducibility. The modified GCE was applied to the simultaneous detection of DA and UA in spiked human urine and gave satisfying recoveries.

Graphical abstract

Schematic of the preparation procedure of GO-ZIF67 and electrochemical reaction mechanisms of UA and DA at the GO-ZIF67-modified glassy carbon electrode (GCE). GO: graphene oxide; ZIF-67: Co(II)-based zeolitic imidazolate framework.


Nanocomposite Scanning electron microscopy Transmission electron microscopy Fourier transform infrared spectroscopy X-ray diffraction Cyclic voltammetry Differential pulse voltammetry Electrochemical sensor Human urine 



This work was supported by the National Nature Foundation of Anhui Province (Grants 1808085 MB31), the Project of Education Department of Anhui Province (KJ2017A506), the key discipline of Anhui Science and Technology University (AKZDXK2015A01) and the Student’s Platform for Innovation and Entrepreneurship Training Program of China (201810879020).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_3025_MOESM1_ESM.doc (1.5 mb)
ESM 1 (DOC 1529 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Chemistry and Material EngineeringAnhui Science and Technology UniversityBengbuChina
  2. 2.College of Resource and EnvironmentAnhui Science and Technology UniversityFengyangChina

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