Microchimica Acta

, 185:458 | Cite as

Graphene oxide wrapped with gold nanorods as a tag in a SERS based immunoassay for the hepatitis B surface antigen

  • Minghuan Liu
  • Chaohui Zheng
  • Malin Cui
  • Xiaoyan Zhang
  • Da-Peng YangEmail author
  • Xiansong WangEmail author
  • Daxiang CuiEmail author
Original Paper


A composite consisting of graphene oxide and gold nanorods (GO-GNRs) was designed for the trace determination of hepatitis B surface antigen (HBsAg) using surface enhanced Raman spectroscopy (SERS). GO contains numerous carboxy and hydroxy groups on its surface and therefore can serve as the substrate for decoration with GNRs and for immobilizing antibody against HBsAg. The GNRs (carrying the SERS probe 2-mercaptopyridine) exhibit high SERS activity, and this improves the sensitivity of the biosensor. The antibody on the GO-GNRs binds HBsAg with high specificity, and it results in excellent selectivity. The SERS signal (measured at 1002 cm−1) increases in the 1–1000 pg·mL−1 HBsAg concentrations range, and the limit of detection is 0.05 pg·mL−1 (at an S/N ratio of 3). The immunoassay achieves the sensitive and selective determination of HBsAg in serum and expands the potential application of GO-GNR based SERS tag in clinical research.

Graphical abstract

A novel graphene oxide-gold nanorod (GO-GNRs) based surface-enhanced Raman scattering (SERS) tag for immunoassay was designed. It allows for sensitive and selective determination of HBsAg in serum. The method is expected to expand the potential application in the environment, in medicine and in food analysis.


Carbon materials Gold nanomaterials Hepatitis B surface antigen Surface-enhanced Raman scattering tag 2-Mercaptopyridine Immunosensor 



This work was supported by the National Natural Science Foundation of China (81472001, 31400851), the Minjiang Scholars Program of Fujian Province, the Tongjiang Scholars Program of Quanzhou City, the Fourth Health Education Joint Development Project of Fujian Province (WKJ-2016-2-36). Natural Science Foundation of Fujian Province (2015 J05030).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2989_MOESM1_ESM.doc (422 kb)
ESM 1 (DOC 422 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The Second Affiliated Hospital of Fujian Medical UniversityQuanzhouChina
  2. 2.College of Chemical Engineering and Materials ScienceQuanzhou Normal UniversityFujian ProvinceChina
  3. 3.Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s HospitalShanghai Jiao Tong University School of MedicineShanghaiChina
  4. 4.Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Department of Instrument Science & Engineering, School of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghaiChina

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