Microchimica Acta

, 185:439 | Cite as

A glassy carbon electrode modified with a composite consisting of gold nanoparticle, reduced graphene oxide and poly(L-arginine) for simultaneous voltammetric determination of dopamine, serotonin and L-tryptophan

  • Md. Zaved H. Khan
  • Xiaoqiang Liu
  • Yunfei Tang
  • Jinhua Zhu
  • Weiping Hu
  • Xiuhua LiuEmail author
Original Paper


A glassy carbon electrode (GCE) was modified with poly(L-arginine) (P-Arg), reduced graphene oxide (rGO) and gold nanoparticle (AuNP) to obtain an electrode for simultaneous determination of dopamine (DA), serotonin (5-HT) and L-tryptophan (L-Trp) in the presence of ascorbic acid (AA). The modified GCE was prepared via subsequent ‘layer-by-layer’ deposition using an electrochemical technique. The surface morphology of the modified electrode was studied by scanning electron microscopy, and electrochemical characterizations were carried out via cyclic voltammetry and electrochemical impedance spectroscopy. The modified electrode showed excellent electrocatalytic activity toward DA, 5-HT and L-Trp at pH 7.0. Figures of merit for the differential pulse voltammetric reponse are as follows: (a) Response to DA is linear in two intervals, viz. 1.0–50 nM and 1.0–50 μM DA concentration range, the typical working voltage is 202 mV (vs. Ag/AgCl), and the detection limit is 1 nM (at an S/N ratio of 3). For 5-HT, the respective data are 10 to 500 nM and 1.0 to 10 μM, 381 mV, and 30 nM. For L-Trp, the respective data are 10–70 nM and 10–100 μM, 719 mV, and 0.1 μM. The modified GCE is fairly selective. It was successfully applied to the simultaneous determination of DA, 5-HT, and L-Trp in spiked urine samples, and high recovery rates were found.

Graphical abstract

Schematic presentation of the voltammetric sensor based on a glassy carbon electrode modified with poly(L-arginine), reduced graphene oxide (rGO) and gold nanoparticle (GCE/P-Arg/ErGO/AuNP) for simultaneous determination of dopamine (DA), serotonin (5-HT) and L-tryptophan (L-Trp).


Electrochemical sensor Nanocomposite Cyclic voltammetry Differential pulse voltammetry Scanning electron microscopy modified electrode Urine analysis 



We are grateful for financial support from the Joint Fund for Fostering Talents of National Natural Science Foundation of China and Henan province (No. U1204304), College Science and technology innovation team program of Henan Province (No. 14IRTSTHN030).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2979_MOESM1_ESM.docx (317 kb)
ESM 1 (DOCX 316 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Md. Zaved H. Khan
    • 1
    • 2
  • Xiaoqiang Liu
    • 1
  • Yunfei Tang
    • 1
  • Jinhua Zhu
    • 1
  • Weiping Hu
    • 1
  • Xiuhua Liu
    • 1
    • 3
    Email author
  1. 1.College of Chemistry and Chemical EngineeringHenan UniversityKaifengChina
  2. 2.Department of Chemical EngineeringJessore University of Science and technologyJessoreBangladesh
  3. 3.Key Laboratory of Natural Medicine and Immune-Engineering of Henan ProvinceKaifengChina

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