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Colorimetric aptasensor for Campylobacter jejuni cells by exploiting the peroxidase like activity of Au@Pd nanoparticles

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Abstract

The authors describe a method for colorimetric determination of Campylobacter jejuni (C. jejuni) in milk samples. It is based on the interaction of a specific DNA aptamer with surface protein in the cell membranes of C. jejuni. Specific binding of the aptamer with the cell membrane leads to an uptake of aptamer from solution. As a result, the concentration of aptamer floating in the solution is reduced. In the presence of large quantities of aptamer, the surface of added Au@Pd nanoparticles (NPs) is covered with aptamer via electrostatic interactions. Hence, they cannot act as a peroxidase mimic and oxidize the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to give a blue product. However, when the aptamer is bound by the target cells, the surface of the NPs is not blocked by aptamer and the NPs exert a strong peroxidase -like activity. Under defined experimental conditions, the intensity of the blue color increases with the concentration of C. jejuni, and as little as 100 CFU·mL−1 can bedetected in milk.

A colorimetric aptasensor for assay Campylobacter jejuni whole cell in food samples was investigated. This assay was designed based on interaction of specific DNA aptamer with surface protein in c. jejuni cell membrane without any modification of aptamer.

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Acknowledgements

The authors thank the research Council of University of Tehran (Grant 28645/01/02) and Tehran University of Medical Sciences for financial support of this work.

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Authors and Affiliations

  1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, 1417466191, Iran

    Zahra Dehghani, Morteza Hosseini, Javad Mohammadnejad & Ali Hossein Rezayan

  2. Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Morteza Hosseini

  3. School of Medical Sciences, University of Tarbiat Modares, Tehran, Iran

    Bita Bakhshi

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  1. Zahra Dehghani
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Correspondence to Morteza Hosseini.

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Dehghani, Z., Hosseini, M., Mohammadnejad, J. et al. Colorimetric aptasensor for Campylobacter jejuni cells by exploiting the peroxidase like activity of Au@Pd nanoparticles. Microchim Acta 185, 448 (2018). https://doi.org/10.1007/s00604-018-2976-2

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